Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210728-1204-10184 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-07-28T12:25:54.452 Number of Tests: 30 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value A1 (+++) (A) * c 15886 (Standard) Escherichia coli 2.25 Escherichia coli 2.21 A2 (+++) (A) * c 15903 (Standard) Pseudomonas aeruginosa 2.15 Pseudomonas aeruginosa 2.01 A3 (+++) (B) * c 15865 (Standard) Enterobacter cloacae 2.21 Enterobacter xiangfangensis 2.15 A4 (+) (B) * c 15870 (Standard) Klebsiella pneumoniae 1.91 Klebsiella pneumoniae 1.84 A5 (+++) (A) * b 14626 (Standard) Escherichia coli 2.22 Escherichia coli 2.19 A6 (+++) (A) * c 15864 (Standard) Burkholderia multivorans 2.19 Burkholderia multivorans 2.16 A7 (+++) (A) * b 14621 (Standard) Escherichia coli 2.15 Escherichia coli 2.14 Result overview table--continued on next page Report created at 2021-07-28T12:28:27 Research Use Only Page 1 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value A8 (+++) (A) * b 14622 (Standard) Escherichia coli 2.23 Escherichia coli 2.14 A9 (+++) (A) * c 15913 (Standard) Citrobacter braakii 2.36 Citrobacter braakii 2.31 A10 (+++) (A) * c 15904-klva (Standard) Klebsiella variicola 2.30 Klebsiella variicola 2.21 A11 (+++) (A) * c 15909-psae (Standard) Pseudomonas aeruginosa 2.22 Pseudomonas aeruginosa 2.21 A12 (+) (B) * c 15908-klpn (Standard) Klebsiella pneumoniae 1.81 Klebsiella pneumoniae 1.73 B1 (+++) (A) * c 15908-psae (Standard) Pseudomonas aeruginosa 2.39 Pseudomonas aeruginosa 2.16 B2 (+++) (A) * c 15909 (Standard) Proteus mirabilis 2.25 Proteus mirabilis 2.15 B3 (+++) (A) * c 15910 (Standard) Proteus mirabilis 2.26 Proteus mirabilis 2.19 B4 (+++) (A) * c 15961 (Standard) Proteus mirabilis 2.08 Proteus mirabilis 2.07 B5 (+++) (A) * b 14613 (Standard) Citrobacter braakii 2.26 Citrobacter braakii 2.22 B6 (+++) (A) * b 14614 (Standard) Citrobacter braakii 2.39 Citrobacter braakii 2.30 B7 (+++) (B) * b 14612 (Standard) Citrobacter freundii 2.16 Citrobacter braakii 2.13 B8 (+++) (A) * b 14616 (Standard) Citrobacter braakii 2.22 Citrobacter braakii 2.22 B9 (+++) (A) * b 14615 (Standard) Citrobacter braakii 2.28 Citrobacter braakii 2.24 B10 (+++) (A) * b 14620 (Standard) Escherichia coli 2.31 Escherichia coli 2.30 B11 (+++) (A) * b 14623 (Standard) Escherichia coli 2.14 Escherichia coli 2.13 Result overview table--continued on next page Report created at 2021-07-28T12:28:27 Research Use Only Page 2 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value B12 (+++) (A) * b 14624 (Standard) Escherichia coli 2.11 Escherichia coli 2.11 C1 (+++) (A) * b 14611 (Standard) Klebsiella oxytoca 2.31 Klebsiella oxytoca 2.14 C2 (+++) (A) * a 14827-sede (Standard) Aeromonas veronii 2.09 Aeromonas veronii 2.08 C3 (+++) (A) * b 14574 (Standard) Escherichia coli 2.30 Escherichia coli 2.22 C4 (+++) (A) * b 14573 (Standard) Escherichia coli 2.32 Escherichia coli 2.24 C5 (+++) (A) * a 14827-bile (Standard) Aeromonas veronii 2.20 Aeromonas veronii 2.12 C6 (-) (C) * xxx (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-07-28T12:28:27 Research Use Only Page 3 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Matching Hints Matched Pattern Comment Aeromonas hydrophila CECT 839T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas hydrophila ssp hydrophila DSM 30187T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas ichthiosmia DSM 6393T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas jandaei CECT 4228T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas media DSM 4881T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas veronii CECT 4199T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas veronii CECT 4257T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas veronii CECT 5761T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas veronii DSM 11576T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas veronii DSM 17676 HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Aeromonas veronii DSM 7386T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Burkholderia anthina DSM 16086T DSM is a member of Burkholderia cepacia complex Burkholderia cenocepacia LMG 12614 HAM is a member of Burkholderia cepacia complex Burkholderia diffusa DSM 23434T DSM is a member of Burkholderia cepacia complex Burkholderia dolosa DSM 16088T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans DSM 13243T DSM is a member of Burkholderia cepacia complex Matching Hints table--continued on next page Report created at 2021-07-28T12:28:27 Research Use Only Page 4 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Matching Hints table--continued from previous page Matched Pattern Comment Burkholderia multivorans LMG 14293 HAM is a member of Burkholderia cepacia complex Burkholderia multivorans VA40328_07 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721a_08 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721b_08 ERL is a member of Burkholderia cepacia complex Burkholderia vietnamiensis LMG 10929T HAM is a member of Burkholderia cepacia complex Citrobacter braakii 20663_2 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter braakii 9314_2 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii 13158_2 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii 22054_1 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii DSM 15979 DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii DSM 30039T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii DSM 30039T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii LMG 21265 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii LMG 3251 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter gillenii CCUG 2749 CCUG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter gillenii DSM 13694T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-07-28T12:28:27 Research Use Only Page 5 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Matching Hints table--continued from previous page Matched Pattern Comment Citrobacter murliniae DSM 13695T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter youngae DSM 17578T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Enterobacter asburiae DSM 17506T DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101087 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101088 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101090 DSM is a member of Enterobacter cloacae complex Enterobacter cloacae 13159_1 CHB is a member of Enterobacter cloacae complex Enterobacter cloacae MB11506_1 CHB is a member of Enterobacter cloacae complex Enterobacter hormaechei ssp hormaechei DSM 12409T DSM is a member of Enterobacter cloacae complex Enterobacter kobei DSM 13645T DSM is a member of Enterobacter cloacae complex Enterobacter xiangfangensis DSM 101093T DSM is a member of Enterobacter cloacae complex Enterobacter xiangfangensis DSM 46348 DSM is a member of Enterobacter cloacae complex Escherichia coli ATCC 25922 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 25922 THL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 35218 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Matching Hints table--continued on next page Report created at 2021-07-28T12:28:27 Research Use Only Page 6 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Matching Hints table--continued from previous page Matched Pattern Comment Escherichia coli DSM 1103_QC DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1576 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 30083T HAM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ESBL_EA_RSS_1528T CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli MB11464_1 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli RV412_A1_2010_06a LBK closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Klebsiella aerogenes 15282_1 CHB synonym of Enterobacter aerogenes Klebsiella aerogenes ATCC 13048T THL synonym of Enterobacter aerogenes Klebsiella oxytoca 35130 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ATCC 700324 THL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ESBL 30298 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_1_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_2_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-07-28T12:28:27 Research Use Only Page 7 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella oxytoca VA20879_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA31877_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_01 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola CC_2123 MCW closely related to Klebsiella pneumoniae Matching Hints table--continued on next page Report created at 2021-07-28T12:28:27 Research Use Only Page 8 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Raoultella ornithinolytica DSM 7464T DSM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MB_18887 CHB Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Report created at 2021-07-28T12:28:27 Research Use Only Page 9 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-07-28T12:28:27 Research Use Only Page 10 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 1 Sample Name: A1 Sample Description: Sample ID: * c 15886 Sample Creation Date/Time: 2021-07-28T12:10:41.474 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 30083T HAM 2.25 562 2 (+++) Escherichia coli MB11464_1 CHB 2.21 562 3 (+++) Escherichia coli Nissl VML 2.18 562 4 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.16 562 5 (+++) Escherichia coli ATCC 25922 THL 2.13 562 6 (+++) Escherichia coli ATCC 35218 CHB 2.10 562 7 (+++) Escherichia coli DSM 682 DSM 2.05 562 8 (+++) Escherichia coli DSM 1576 DSM 2.05 562 9 (+++) Escherichia coli DH5alpha BRL 2.03 562 10 (+) Escherichia coli DSM 1103_QC DSM 1.95 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 11 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 2 Sample Name: A2 Sample Description: Sample ID: * c 15903 Sample Creation Date/Time: 2021-07-28T12:10:41.475 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa 19955_1 CHB 2.15 287 2 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.01 287 3 (+) Pseudomonas aeruginosa 8147_2 CHB 1.85 287 4 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.75 287 5 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.66 198616 6 (-) Pseudomonas aeruginosa DSM 1117 DSM 1.66 287 7 (-) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.60 287 8 (-) Pseudomonas aeruginosa LMG 8029 LMG 1.42 287 9 (-) Pseudomonas aeruginosa A07_08_Pudu FLR 1.37 287 10 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.32 287 Report created at 2021-07-28T12:28:27 Research Use Only Page 12 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 3 Sample Name: A3 Sample Description: Sample ID: * c 15865 Sample Creation Date/Time: 2021-07-28T12:10:41.475 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae 13159_1 CHB 2.21 550 2 (+++) Enterobacter xiangfangensis DSM 101093T DSM 2.15 547 3 (+++) Enterobacter xiangfangensis DSM 46348 DSM 2.11 547 4 (+++) Enterobacter cloacae MB11506_1 CHB 2.11 550 5 (+++) Enterobacter hormaechei ssp hormaechei DSM 12409T DSM 2.11 301105 6 (+++) Enterobacter bugandensis DSM 101087 DSM 2.04 547 7 (+++) Enterobacter asburiae DSM 17506T DSM 2.02 61645 8 (+++) Enterobacter bugandensis DSM 101088 DSM 2.01 547 9 (+++) Enterobacter kobei DSM 13645T DSM 2.01 208224 10 (+++) Enterobacter bugandensis DSM 101090 DSM 2.01 547 Report created at 2021-07-28T12:28:27 Research Use Only Page 13 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 4 Sample Name: A4 Sample Description: Sample ID: * c 15870 Sample Creation Date/Time: 2021-07-28T12:10:41.476 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.91 573 2 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.84 72407 3 (+) Klebsiella variicola DSM 15968T HAM 1.77 244366 4 (-) Escherichia coli MB11464_1 CHB 1.62 562 5 (-) Proteus mirabilis 13210_1 CHB 1.61 584 6 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.60 72407 7 (-) Proteus mirabilis DSM 50903 DSM 1.59 584 8 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.59 72407 9 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.58 574 10 (-) Klebsiella pneumoniae 37585 PFM 1.58 573 Report created at 2021-07-28T12:28:27 Research Use Only Page 14 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 5 Sample Name: A5 Sample Description: Sample ID: * b 14626 Sample Creation Date/Time: 2021-07-28T12:10:41.476 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.22 562 2 (+++) Escherichia coli DH5alpha BRL 2.19 562 3 (+++) Escherichia coli MB11464_1 CHB 2.19 562 4 (+++) Escherichia coli DSM 30083T HAM 2.14 562 5 (+++) Escherichia coli Nissl VML 2.11 562 6 (+++) Escherichia coli ATCC 25922 THL 2.09 562 7 (+++) Escherichia coli ATCC 35218 CHB 2.03 562 8 (+++) Escherichia coli DSM 682 DSM 2.00 562 9 (+) Escherichia coli DSM 1576 DSM 1.99 562 10 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.93 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 15 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 6 Sample Name: A6 Sample Description: Sample ID: * c 15864 Sample Creation Date/Time: 2021-07-28T12:10:41.477 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Burkholderia multivorans DSM 13243T DSM 2.19 87883 2 (+++) Burkholderia multivorans VA40328_07 ERL 2.16 87883 3 (+++) Burkholderia multivorans LMG 14293 HAM 2.14 87883 4 (+++) Burkholderia multivorans VA40721a_08 ERL 2.12 87883 5 (+) Burkholderia dolosa DSM 16088T DSM 1.93 152500 6 (+) Burkholderia multivorans VA40721b_08 ERL 1.91 87883 7 (+) Burkholderia vietnamiensis LMG 10929T HAM 1.86 60552 8 (+) Burkholderia anthina DSM 16086T DSM 1.77 179879 9 (+) Burkholderia cenocepacia LMG 12614 HAM 1.73 95486 10 (-) Burkholderia diffusa DSM 23434T DSM 1.69 87882 Report created at 2021-07-28T12:28:27 Research Use Only Page 16 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 7 Sample Name: A7 Sample Description: Sample ID: * b 14621 Sample Creation Date/Time: 2021-07-28T12:10:41.477 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.15 562 2 (+++) Escherichia coli ATCC 25922 THL 2.14 562 3 (+++) Escherichia coli MB11464_1 CHB 2.13 562 4 (+++) Escherichia coli DSM 30083T HAM 2.12 562 5 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.07 562 6 (+++) Escherichia coli DSM 1576 DSM 2.03 562 7 (+++) Escherichia coli DSM 682 DSM 2.00 562 8 (+) Escherichia coli ATCC 35218 CHB 1.99 562 9 (+) Escherichia coli DSM 1103_QC DSM 1.97 562 10 (+) Escherichia coli ATCC 25922 CHB 1.96 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 17 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 8 Sample Name: A8 Sample Description: Sample ID: * b 14622 Sample Creation Date/Time: 2021-07-28T12:10:41.478 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.23 562 2 (+++) Escherichia coli ATCC 25922 THL 2.14 562 3 (+++) Escherichia coli MB11464_1 CHB 2.14 562 4 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.13 562 5 (+++) Escherichia coli DSM 682 DSM 2.12 562 6 (+++) Escherichia coli DSM 30083T HAM 2.11 562 7 (+++) Escherichia coli DSM 1103_QC DSM 2.04 562 8 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.00 562 9 (+++) Escherichia coli Nissl VML 2.00 562 10 (+) Escherichia coli DSM 1576 DSM 1.98 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 18 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 9 Sample Name: A9 Sample Description: Sample ID: * c 15913 Sample Creation Date/Time: 2021-07-28T12:10:41.478 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter braakii 9314_2 CHB 2.36 57706 2 (+++) Citrobacter braakii 20663_2 CHB 2.31 57706 3 (+++) Citrobacter freundii 22054_1 CHB 2.12 546 4 (+++) Citrobacter freundii 13158_2 CHB 2.05 546 5 (+++) Citrobacter freundii DSM 15979 DSM 2.02 546 6 (+) Citrobacter youngae DSM 17578T HAM 1.99 133448 7 (+) Citrobacter freundii DSM 30039T HAM 1.98 546 8 (+) Citrobacter freundii DSM 30039T DSM 1.97 546 9 (+) Citrobacter freundii LMG 21265 LMG 1.96 546 10 (+) Citrobacter murliniae DSM 13695T HAM 1.94 67829 Report created at 2021-07-28T12:28:27 Research Use Only Page 19 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 10 Sample Name: A10 Sample Description: Sample ID: * c 15904-klva Sample Creation Date/Time: 2021-07-28T12:10:41.479 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola CC_2053 MCW 2.30 244366 2 (+++) Klebsiella variicola DSM 15968T DSM 2.21 244366 3 (+++) Klebsiella variicola 150519_03 SAH 2.20 244366 4 (+++) Klebsiella variicola CC_2123 MCW 2.20 244366 5 (+++) Klebsiella variicola NY_1064 MCW 2.18 244366 6 (+++) Klebsiella variicola TC_4131 MCW 2.18 244366 7 (+++) Klebsiella variicola NY_1125 MCW 2.17 244366 8 (+++) Klebsiella variicola 150416_01 BRUS 2.17 244366 9 (+++) Klebsiella variicola 37924 PFM 2.13 244366 10 (+++) Klebsiella variicola 150519_01 SAH 2.12 244366 Report created at 2021-07-28T12:28:27 Research Use Only Page 20 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 11 Sample Name: A11 Sample Description: Sample ID: * c 15909-psae Sample Creation Date/Time: 2021-07-28T12:10:41.479 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa 19955_1 CHB 2.22 287 2 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.21 287 3 (+++) Pseudomonas aeruginosa 8147_2 CHB 2.19 287 4 (+++) Pseudomonas aeruginosa DSM 50071T HAM 2.09 287 5 (+++) Pseudomonas aeruginosa DSM 1117 DSM 2.06 287 6 (+) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.98 287 7 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.73 287 8 (+) Pseudomonas aeruginosa LMG 8029 LMG 1.70 287 9 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.60 198616 10 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.49 287 Report created at 2021-07-28T12:28:27 Research Use Only Page 21 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 12 Sample Name: A12 Sample Description: Sample ID: * c 15908-klpn Sample Creation Date/Time: 2021-07-28T12:10:41.480 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.81 573 2 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.73 72407 3 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.58 72407 4 (-) Klebsiella pneumoniae 37585 PFM 1.52 573 5 (-) Klebsiella variicola 150416_01 BRUS 1.51 244366 6 (-) Klebsiella variicola CC_2053 MCW 1.48 244366 7 (-) Klebsiella aerogenes 15282_1 CHB 1.45 28451 8 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.44 574 9 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.43 574 10 (-) Klebsiella variicola 150519_03 SAH 1.43 244366 Report created at 2021-07-28T12:28:27 Research Use Only Page 22 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 13 Sample Name: B1 Sample Description: Sample ID: * c 15908-psae Sample Creation Date/Time: 2021-07-28T12:10:41.480 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.39 287 2 (+++) Pseudomonas aeruginosa 8147_2 CHB 2.16 287 3 (+++) Pseudomonas aeruginosa DSM 1117 DSM 2.14 287 4 (+++) Pseudomonas aeruginosa DSM 50071T HAM 2.11 287 5 (+++) Pseudomonas aeruginosa DSM 50071T_QC DSM 2.03 287 6 (+++) Pseudomonas aeruginosa 19955_1 CHB 2.00 287 7 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.97 287 8 (+) Pseudomonas aeruginosa LMG 8029 LMG 1.93 287 9 (+) Pseudomonas aeruginosa DSM 1128 DSM 1.93 287 10 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.65 198616 Report created at 2021-07-28T12:28:27 Research Use Only Page 23 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 14 Sample Name: B2 Sample Description: Sample ID: * c 15909 Sample Creation Date/Time: 2021-07-28T12:10:41.481 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Proteus mirabilis DSM 50903 DSM 2.25 584 2 (+++) Proteus mirabilis DSM 788 DSM 2.15 584 3 (+++) Proteus mirabilis 13210_1 CHB 2.13 584 4 (+++) Proteus mirabilis DSM 18254 DSM 2.13 584 5 (+++) Proteus mirabilis DSM 30115 DSM 2.10 584 6 (+++) Proteus mirabilis 9482_2 CHB 2.09 584 7 (+++) Proteus mirabilis (PX) 22086112 MLD 2.05 584 8 (+) Proteus mirabilis DSM 46227 DSM 1.95 584 9 (+) Proteus mirabilis RV412_A1_2010_06b LBK 1.92 584 10 (-) Pseudomonas aeruginosa ATCC 27853 THL 1.46 287 Report created at 2021-07-28T12:28:27 Research Use Only Page 24 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 15 Sample Name: B3 Sample Description: Sample ID: * c 15910 Sample Creation Date/Time: 2021-07-28T12:10:41.481 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Proteus mirabilis 9482_2 CHB 2.26 584 2 (+++) Proteus mirabilis DSM 50903 DSM 2.19 584 3 (+++) Proteus mirabilis DSM 788 DSM 2.17 584 4 (+++) Proteus mirabilis DSM 18254 DSM 2.16 584 5 (+++) Proteus mirabilis 13210_1 CHB 2.11 584 6 (+++) Proteus mirabilis DSM 30115 DSM 2.11 584 7 (+++) Proteus mirabilis DSM 46227 DSM 2.09 584 8 (+++) Proteus mirabilis (PX) 22086112 MLD 2.03 584 9 (+) Proteus mirabilis RV412_A1_2010_06b LBK 1.96 584 10 (-) Clostridium difficile MB_7476_05 THL 1.42 1496 Report created at 2021-07-28T12:28:27 Research Use Only Page 25 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 16 Sample Name: B4 Sample Description: Sample ID: * c 15961 Sample Creation Date/Time: 2021-07-28T12:10:41.482 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Proteus mirabilis DSM 50903 DSM 2.08 584 2 (+++) Proteus mirabilis 9482_2 CHB 2.07 584 3 (+++) Proteus mirabilis DSM 18254 DSM 2.02 584 4 (+++) Proteus mirabilis DSM 788 DSM 2.00 584 5 (+++) Proteus mirabilis 13210_1 CHB 2.00 584 6 (+) Proteus mirabilis DSM 46227 DSM 1.97 584 7 (+) Proteus mirabilis DSM 30115 DSM 1.96 584 8 (+) Proteus mirabilis (PX) 22086112 MLD 1.90 584 9 (+) Proteus mirabilis RV412_A1_2010_06b LBK 1.77 584 10 (-) Escherichia coli MB11464_1 CHB 1.41 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 26 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 17 Sample Name: B5 Sample Description: Sample ID: * b 14613 Sample Creation Date/Time: 2021-07-28T12:10:41.482 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter braakii 9314_2 CHB 2.26 57706 2 (+++) Citrobacter braakii 20663_2 CHB 2.22 57706 3 (+++) Citrobacter freundii 22054_1 CHB 2.18 546 4 (+++) Citrobacter gillenii DSM 13694T DSM 2.17 67828 5 (+++) Citrobacter freundii DSM 15979 DSM 2.16 546 6 (+++) Citrobacter gillenii CCUG 2749 CCUG 2.12 67828 7 (+++) Citrobacter freundii DSM 30039T DSM 2.10 546 8 (+++) Citrobacter freundii LMG 3251 LMG 2.09 546 9 (+++) Citrobacter freundii DSM 30039T HAM 2.08 546 10 (+++) Citrobacter freundii LMG 21265 LMG 2.06 546 Report created at 2021-07-28T12:28:27 Research Use Only Page 27 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 18 Sample Name: B6 Sample Description: Sample ID: * b 14614 Sample Creation Date/Time: 2021-07-28T12:10:41.483 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter braakii 9314_2 CHB 2.39 57706 2 (+++) Citrobacter braakii 20663_2 CHB 2.30 57706 3 (+++) Citrobacter freundii 22054_1 CHB 2.14 546 4 (+++) Citrobacter freundii DSM 15979 DSM 2.06 546 5 (+++) Citrobacter murliniae DSM 13695T HAM 2.05 67829 6 (+++) Citrobacter gillenii CCUG 2749 CCUG 2.05 67828 7 (+++) Citrobacter freundii DSM 30039T HAM 2.02 546 8 (+++) Citrobacter gillenii DSM 13694T DSM 2.02 67828 9 (+++) Citrobacter freundii 13158_2 CHB 2.01 546 10 (+) Citrobacter youngae DSM 17578T HAM 1.99 133448 Report created at 2021-07-28T12:28:27 Research Use Only Page 28 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 19 Sample Name: B7 Sample Description: Sample ID: * b 14612 Sample Creation Date/Time: 2021-07-28T12:10:41.483 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter freundii 22054_1 CHB 2.16 546 2 (+++) Citrobacter braakii 9314_2 CHB 2.13 57706 3 (+++) Citrobacter braakii 20663_2 CHB 2.07 57706 4 (+++) Citrobacter freundii DSM 30039T HAM 2.07 546 5 (+) Citrobacter freundii LMG 3251 LMG 1.97 546 6 (+) Citrobacter youngae DSM 17578T HAM 1.94 133448 7 (+) Citrobacter freundii LMG 21265 LMG 1.93 546 8 (+) Citrobacter freundii DSM 15979 DSM 1.88 546 9 (+) Citrobacter freundii 13158_2 CHB 1.86 546 10 (+) Citrobacter freundii DSM 30039T DSM 1.86 546 Report created at 2021-07-28T12:28:27 Research Use Only Page 29 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 20 Sample Name: B8 Sample Description: Sample ID: * b 14616 Sample Creation Date/Time: 2021-07-28T12:10:41.484 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter braakii 20663_2 CHB 2.22 57706 2 (+++) Citrobacter braakii 9314_2 CHB 2.22 57706 3 (+++) Citrobacter freundii 22054_1 CHB 2.22 546 4 (+++) Citrobacter freundii DSM 15979 DSM 2.06 546 5 (+++) Citrobacter youngae DSM 17578T HAM 2.03 133448 6 (+++) Citrobacter freundii DSM 30039T DSM 2.01 546 7 (+++) Citrobacter freundii LMG 3251 LMG 2.00 546 8 (+++) Citrobacter gillenii DSM 13694T DSM 2.00 67828 9 (+) Citrobacter freundii 13158_2 CHB 1.99 546 10 (+) Citrobacter freundii LMG 21265 LMG 1.99 546 Report created at 2021-07-28T12:28:27 Research Use Only Page 30 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 21 Sample Name: B9 Sample Description: Sample ID: * b 14615 Sample Creation Date/Time: 2021-07-28T12:10:41.484 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter braakii 20663_2 CHB 2.28 57706 2 (+++) Citrobacter braakii 9314_2 CHB 2.24 57706 3 (+++) Citrobacter freundii 22054_1 CHB 2.15 546 4 (+++) Citrobacter gillenii DSM 13694T DSM 2.04 67828 5 (+++) Citrobacter freundii DSM 15979 DSM 2.03 546 6 (+++) Citrobacter freundii DSM 30039T HAM 2.03 546 7 (+++) Citrobacter freundii 13158_2 CHB 2.02 546 8 (+) Citrobacter gillenii CCUG 2749 CCUG 1.98 67828 9 (+) Citrobacter youngae DSM 17578T HAM 1.97 133448 10 (+) Citrobacter freundii LMG 21265 LMG 1.96 546 Report created at 2021-07-28T12:28:27 Research Use Only Page 31 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 22 Sample Name: B10 Sample Description: Sample ID: * b 14620 Sample Creation Date/Time: 2021-07-28T12:10:41.485 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.31 562 2 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.30 562 3 (+++) Escherichia coli ATCC 25922 THL 2.18 562 4 (+++) Escherichia coli DSM 30083T HAM 2.12 562 5 (+++) Escherichia coli Nissl VML 2.08 562 6 (+++) Escherichia coli DSM 1576 DSM 2.07 562 7 (+++) Escherichia coli DSM 682 DSM 2.05 562 8 (+++) Escherichia coli MB11464_1 CHB 2.03 562 9 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.96 562 10 (+) Escherichia coli ATCC 35218 CHB 1.93 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 32 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 23 Sample Name: B11 Sample Description: Sample ID: * b 14623 Sample Creation Date/Time: 2021-07-28T12:10:41.485 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.14 562 2 (+++) Escherichia coli DH5alpha BRL 2.13 562 3 (+) Escherichia coli MB11464_1 CHB 1.98 562 4 (+) Escherichia coli DSM 682 DSM 1.95 562 5 (+) Escherichia coli DSM 30083T HAM 1.94 562 6 (+) Escherichia coli ATCC 25922 THL 1.90 562 7 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.90 562 8 (+) Escherichia coli Nissl VML 1.90 562 9 (+) Escherichia coli ATCC 35218 CHB 1.90 562 10 (+) Escherichia coli DSM 1576 DSM 1.85 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 33 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 24 Sample Name: B12 Sample Description: Sample ID: * b 14624 Sample Creation Date/Time: 2021-07-28T12:10:41.486 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli ATCC 35218 CHB 2.11 562 2 (+++) Escherichia coli MB11464_1 CHB 2.11 562 3 (+++) Escherichia coli DH5alpha BRL 2.09 562 4 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.07 562 5 (+++) Escherichia coli DSM 30083T HAM 2.02 562 6 (+++) Escherichia coli ATCC 25922 THL 2.00 562 7 (+) Escherichia coli Nissl VML 1.99 562 8 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.96 562 9 (+) Escherichia coli DSM 1103_QC DSM 1.95 562 10 (+) Escherichia coli DSM 682 DSM 1.95 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 34 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 25 Sample Name: C1 Sample Description: Sample ID: * b 14611 Sample Creation Date/Time: 2021-07-28T12:10:41.486 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca ATCC 700324 THL 2.31 571 2 (+++) Klebsiella oxytoca VA20876_1_09 ERL 2.14 571 3 (+++) Klebsiella oxytoca VA20879_09 ERL 2.10 571 4 (+++) Klebsiella oxytoca 35130 PFM 2.06 571 5 (+++) Klebsiella oxytoca VA20876_2_09 ERL 2.02 571 6 (+) Raoultella ornithinolytica MB_18887 CHB 1.96 54291 7 (+) Klebsiella oxytoca ESBL 30298 PFM 1.96 571 8 (+) Klebsiella oxytoca VA31877_09 ERL 1.94 571 9 (+) Klebsiella aerogenes ATCC 13048T THL 1.93 28451 10 (+) Raoultella ornithinolytica DSM 7464T DSM 1.92 54291 Report created at 2021-07-28T12:28:27 Research Use Only Page 35 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 26 Sample Name: C2 Sample Description: Sample ID: * a 14827-sede Sample Creation Date/Time: 2021-07-28T12:10:41.487 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Aeromonas veronii DSM 17676 HAM 2.09 654 2 (+++) Aeromonas veronii CECT 5761T DSM 2.08 654 3 (+++) Aeromonas ichthiosmia DSM 6393T HAM 2.04 654 4 (+) Aeromonas veronii CECT 4257T DSM 1.96 654 5 (+) Aeromonas veronii CECT 4199T DSM 1.93 654 6 (+) Aeromonas veronii DSM 11576T HAM 1.93 654 7 (+) Aeromonas jandaei CECT 4228T DSM 1.92 650 8 (+) Aeromonas media DSM 4881T HAM 1.91 651 9 (+) Aeromonas hydrophila CECT 839T DSM 1.91 644 10 (+) Aeromonas hydrophila ssp hydrophila DSM 30187T DSM 1.87 196023 Report created at 2021-07-28T12:28:27 Research Use Only Page 36 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 27 Sample Name: C3 Sample Description: Sample ID: * b 14574 Sample Creation Date/Time: 2021-07-28T12:10:41.488 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 682 DSM 2.30 562 2 (+++) Escherichia coli DH5alpha BRL 2.22 562 3 (+++) Escherichia coli DSM 1576 DSM 2.14 562 4 (+++) Escherichia coli ATCC 25922 THL 2.05 562 5 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.05 562 6 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.03 562 7 (+++) Escherichia coli MB11464_1 CHB 2.03 562 8 (+) Escherichia coli ATCC 25922 CHB 1.97 562 9 (+) Escherichia coli DSM 1103_QC DSM 1.96 562 10 (+) Escherichia coli ATCC 35218 CHB 1.93 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 37 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 28 Sample Name: C4 Sample Description: Sample ID: * b 14573 Sample Creation Date/Time: 2021-07-28T12:10:41.488 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 682 DSM 2.32 562 2 (+++) Escherichia coli DH5alpha BRL 2.24 562 3 (+++) Escherichia coli ATCC 25922 THL 2.20 562 4 (+++) Escherichia coli DSM 1576 DSM 2.18 562 5 (+++) Escherichia coli DSM 30083T HAM 2.18 562 6 (+++) Escherichia coli MB11464_1 CHB 2.18 562 7 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.15 562 8 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.09 562 9 (+++) Escherichia coli ATCC 25922 CHB 2.08 562 10 (+++) Escherichia coli DSM 1103_QC DSM 2.04 562 Report created at 2021-07-28T12:28:27 Research Use Only Page 38 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 29 Sample Name: C5 Sample Description: Sample ID: * a 14827-bile Sample Creation Date/Time: 2021-07-28T12:10:41.489 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Aeromonas veronii CECT 5761T DSM 2.20 654 2 (+++) Aeromonas veronii DSM 17676 HAM 2.12 654 3 (+++) Aeromonas hydrophila CECT 839T DSM 2.10 644 4 (+++) Aeromonas veronii CECT 4257T DSM 2.03 654 5 (+++) Aeromonas ichthiosmia DSM 6393T HAM 2.02 654 6 (+++) Aeromonas veronii CECT 4199T DSM 2.01 654 7 (+++) Aeromonas jandaei CECT 4228T DSM 2.00 650 8 (+) Aeromonas media DSM 4881T HAM 1.92 651 9 (+) Aeromonas veronii DSM 11576T HAM 1.91 654 10 (+) Aeromonas veronii DSM 7386T HAM 1.90 654 Report created at 2021-07-28T12:28:27 Research Use Only Page 39 of 40 Run Identifier: 210728-1204-10184 Run Creation Date/Time: 2021-07-28T12:25:54.452 Sample 30 Sample Name: C6 Sample Description: Sample ID: * xxx Sample Creation Date/Time: 2021-07-28T12:10:41.489 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-07-28T12:28:27 Research Use Only Page 40 of 40