Bruker MALDI Biotyper 
Identification Results 


Run Info: 
Run Identifier: 210129-1120-10184 
Comment: 
Operator: maldi@FLEX-PC 
Run Creation Date/Time: 2021-01-29T11:37:01.393 
Number of Tests: 10 
Type: Standard 
BTS-QC: not present 
BTS-QC Position: 
Instrument ID: 269944.01064 
Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 
Result Overview 

Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
D1 
(+++) (A) 
* A 1795 
(Standard) Klebsiella pneumoniae 2.41 Klebsiella pneumoniae 2.34 
D2 
(+++) (A) 
* A 1823 
(Standard) Klebsiella pneumoniae 2.12 Klebsiella pneumoniae 2.06 
D3 
(+) (B) 
* B 1725 
(Standard) Klebsiella variicola 1.93 Klebsiella pneumoniae 1.80 
D4 
(+) (B) 
* B 1752 
(Standard) Klebsiella pneumoniae 1.88 Klebsiella pneumoniae 1.84 
D5 
(+++) (A) 
* B 1717 
(Standard) Klebsiella pneumoniae 2.34 Klebsiella pneumoniae 2.32 
D6 
(+++) (A) 
* B 1713 
(Standard) Klebsiella pneumoniae 2.09 Klebsiella pneumoniae 2.07 
D7 
(+++) (A) 
* B 1696 
(Standard) Klebsiella pneumoniae 2.16 Klebsiella pneumoniae 2.09 
Result overview table--continued on next page 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 

Result overview table--continued from previous page 
Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
D8 
(+++) (A) 
* C 1987 
(Standard) Klebsiella pneumoniae 2.41 Klebsiella pneumoniae 2.35 
D9 
(+++) (A) 
* C 2156 
(Standard) Klebsiella variicola 2.21 Klebsiella variicola 2.16 
D10 
(-) (C) 
* C XX 
(Standard) 
No Organism Identification 
Possible 1.34 No Organism Identification 
Possible 1.28 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 

Matching Hints 

Matched Pattern Comment 
Enterobacter cloacae 13159_1 
CHB is a member of Enterobacter cloacae complex 
Escherichia coli 
ESBL_EA_RSS_1528T CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Klebsiella pneumoniae 37585 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 37595 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 
RV_BA_03_B LBK closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae CCM 5792T CCM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T DSM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T HAM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae 9295_1 CHB closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T 
HAM 
closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T_QC 
DSM 
closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
rhinoscleromatis DSM 16231T 
HAM 
closely related to Klebsiella variicola 
Klebsiella variicola 150416_01 
BRUS closely related to Klebsiella pneumoniae 
Klebsiella variicola 150519_03 
SAH closely related to Klebsiella pneumoniae 
Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae 
Matching Hints table--continued on next page 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Klebsiella variicola CC_2053 
MCW closely related to Klebsiella pneumoniae 
Klebsiella variicola CC_2123 
MCW closely related to Klebsiella pneumoniae 
Klebsiella variicola DSM 
15968T DSM closely related to Klebsiella pneumoniae 
Klebsiella variicola DSM 
15968T HAM closely related to Klebsiella pneumoniae 
Klebsiella variicola NY_1036 
MCW closely related to Klebsiella pneumoniae 
Klebsiella variicola NY_1064 
MCW closely related to Klebsiella pneumoniae 
Klebsiella variicola NY_1125 
MCW closely related to Klebsiella pneumoniae 
Klebsiella variicola TC_4131 
MCW closely related to Klebsiella pneumoniae 
Pseudomonas congelans DSM 
14939T HAM is a member of Pseudomonas fluorescens group 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 

Meaning of Score Values 

Range Interpretation Symbols Color 
2.00 - 3.00 High-confidence identification (+++) green 
1.70 - 1.99 Low-confidence identification (+) yellow 
0.00 - 1.69 No Organism Identification Possible (-) red 

Meaning of Consistency Categories (A - C) 

Category Interpretation 
(A) 
High consistency: The best match is a high-confidence identification. The second-best match is (1) a 
high-confidence identification in which the species is identical to the best match, (2) a low-confidence 
identification in which the species or genus is identical to the best match, or (3) a non-identification. 
(B) 
Low consistency: The requirements for high consistency are not met. The best match is a high- or low-
confidence identification. The second-best match is (1) a high- or low-confidence identification in 
which the genus is identical to the best match or (2) a non-identification. 
(C) No consistency: The requirements for high or low consistency are not met. 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 


Sample 1 

Sample Name: D1 
Sample Description: 
Sample ID: * A 1795 
Sample Creation Date/Time: 2021-01-29T11:22:29.961 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.41 72407 
2 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.34 72407 
3 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.33 574 
4 
(+++) Klebsiella pneumoniae 37585 PFM 2.31 573 
5 
(+++) Klebsiella variicola 150416_01 BRUS 2.29 244366 
6 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.28 72407 
7 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.25 574 
8 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.22 573 
9 
(+++) Klebsiella variicola CC_2053 MCW 2.17 244366 
10 
(+++) Klebsiella pneumoniae 37595 PFM 2.15 573 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 


Sample 2 

Sample Name: D2 
Sample Description: 
Sample ID: * A 1823 
Sample Creation Date/Time: 2021-01-29T11:22:29.962 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.12 72407 
2 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.06 72407 
3 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.00 574 
4 
(+) Klebsiella pneumoniae RV_BA_03_B LBK 1.97 573 
5 
(+) Klebsiella pneumoniae 37585 PFM 1.89 573 
6 
(+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.87 72407 
7 
(+) Klebsiella variicola CC_2053 MCW 1.84 244366 
8 
(+) Klebsiella variicola 150416_01 BRUS 1.83 244366 
9 
(+) Klebsiella pneumoniae 37595 PFM 1.73 573 
10 
(+) Klebsiella variicola 37924 PFM 1.72 244366 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 


Sample 3 

Sample Name: D3 
Sample Description: 
Sample ID: * B 1725 
Sample Creation Date/Time: 2021-01-29T11:22:29.963 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based B 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+) Klebsiella variicola DSM 15968T HAM 1.93 244366 
2 
(+) Klebsiella pneumoniae RV_BA_03_B LBK 1.80 573 
3 
(-) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.67 574 
4 
(-) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.66 72407 
5 
(-) Klebsiella variicola 150519_03 SAH 1.66 244366 
6 
(-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.63 574 
7 
(-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.62 72407 
8 
(-) Klebsiella variicola NY_1036 MCW 1.60 244366 
9 
(-) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.55 39831 
10 
(-) Klebsiella variicola DSM 15968T DSM 1.52 244366 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 


Sample 4 

Sample Name: D4 
Sample Description: 
Sample ID: * B 1752 
Sample Creation Date/Time: 2021-01-29T11:22:29.963 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based B 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+) Klebsiella pneumoniae RV_BA_03_B LBK 1.88 573 
2 
(+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.84 574 
3 
(+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.76 39831 
4 
(+) Klebsiella variicola DSM 15968T HAM 1.75 244366 
5 
(+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.72 72407 
6 
(-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.69 72407 
7 
(-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.62 574 
8 
(-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.58 72407 
9 
(-) Klebsiella variicola DSM 15968T DSM 1.55 244366 
10 
(-) Enterobacter cloacae 13159_1 CHB 1.54 550 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 


Sample 5 

Sample Name: D5 
Sample Description: 
Sample ID: * B 1717 
Sample Creation Date/Time: 2021-01-29T11:22:29.963 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.34 72407 
2 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.32 574 
3 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.27 72407 
4 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.26 72407 
5 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.26 574 
6 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.24 573 
7 
(+++) Klebsiella pneumoniae 37585 PFM 2.17 573 
8 
(+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.08 39831 
9 
(+++) Klebsiella variicola 150519_03 SAH 2.07 244366 
10 
(+++) Klebsiella variicola DSM 15968T HAM 2.05 244366 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 


Sample 6 

Sample Name: D6 
Sample Description: 
Sample ID: * B 1713 
Sample Creation Date/Time: 2021-01-29T11:22:29.964 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.09 573 
2 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.07 574 
3 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.00 72407 
4 
(+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.97 72407 
5 
(+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.90 574 
6 
(+) Klebsiella variicola DSM 15968T HAM 1.85 244366 
7 
(+) Klebsiella pneumoniae 37595 PFM 1.84 573 
8 
(+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.80 72407 
9 
(+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.75 39831 
10 
(-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.69 574 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 


Sample 7 

Sample Name: D7 
Sample Description: 
Sample ID: * B 1696 
Sample Creation Date/Time: 2021-01-29T11:22:29.964 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.16 573 
2 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.09 574 
3 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.08 72407 
4 
(+++) Klebsiella variicola DSM 15968T HAM 2.06 244366 
5 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.00 574 
6 
(+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.99 72407 
7 
(+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.87 72407 
8 
(+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.84 39831 
9 
(+) Klebsiella pneumoniae 37585 PFM 1.83 573 
10 
(+) Klebsiella pneumoniae 37595 PFM 1.83 573 

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Run Identifier: 210129-1120-10184 Run Creation Date/Time: 2021-01-29T11:37:01.393 


Sample 8 

Sample Name: D8 
Sample Description: 
Sample ID: * C 1987 
Sample Creation Date/Time: 2021-01-29T11:22:29.965 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.41 72407 
2 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.35 573 
3 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.35 72407 
4 
(+++) Klebsiella pneumoniae 37585 PFM 2.27 573 
5 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.23 72407 
6 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.22 574 
7 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.18 574 
8 
(+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.06 39831 
9 
(+++) Klebsiella pneumoniae 37595 PFM 2.03 573 
10 
(+) Klebsiella variicola DSM 15968T DSM 1.97 244366 

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Sample 9 

Sample Name: D9 
Sample Description: 
Sample ID: * C 2156 
Sample Creation Date/Time: 2021-01-29T11:22:29.965 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella variicola DSM 15968T DSM 2.21 244366 
2 
(+++) Klebsiella variicola DSM 15968T HAM 2.16 244366 
3 
(+++) Klebsiella variicola CC_2053 MCW 2.07 244366 
4 
(+++) Klebsiella variicola 150519_03 SAH 2.07 244366 
5 
(+++) Klebsiella variicola CC_2123 MCW 2.00 244366 
6 
(+) Klebsiella variicola NY_1125 MCW 1.98 244366 
7 
(+) Klebsiella variicola TC_4131 MCW 1.96 244366 
8 
(+) Klebsiella variicola 37924 PFM 1.95 244366 
9 
(+) Klebsiella variicola 150416_01 BRUS 1.94 244366 
10 
(+) Klebsiella variicola NY_1064 MCW 1.93 244366 

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Sample 10 

Sample Name: D10 
Sample Description: 
Sample ID: * C XX 
Sample Creation Date/Time: 2021-01-29T11:22:29.966 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based C 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(-) Mortierella polygonia CBS 685_71T CBS 1.34 4855 
2 
(-) Filifactor villosus 1051_NCTC 11220T BOG 1.28 29374 
3 
(-) Morganella morganii 9544_1 CHB 1.22 582 
4 
(-) Lactobacillus sakei DSM 20494 DSM 1.20 1599 
5 
(-) Pseudomonas congelans DSM 14939T HAM 1.19 200452 
6 
(-) Morganella morganii ssp sibonii Mb19277_2 CHB 1.18 180435 
7 
(-) Lactobacillus sakei ssp sakei DSM 20017T DSM 1.18 214326 
8 
(-) Escherichia coli ESBL_EA_RSS_1528T CHB 1.18 562 
9 
(-) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.17 72407 
10 
(-) Aromatoleum anaerobicus LuFRes1 MPB 1.17 182180 

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