Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210205-0947-10184 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-02-05T10:10:09.172 Number of Tests: 17 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value D1 (+++) (A) * A 2386 (Standard) Klebsiella pneumoniae 2.23 Klebsiella pneumoniae 2.20 D2 (+) (B) * A 2387 (Standard) Klebsiella variicola 1.83 Klebsiella pneumoniae 1.80 D3 (+++) (A) * A 2389 (Standard) Klebsiella variicola 2.33 Klebsiella variicola 2.33 D4 (+++) (A) * A 2403-11 (Standard) Klebsiella pneumoniae 2.16 Klebsiella pneumoniae 2.14 D5 (+++) (A) * A 2403-12 (Standard) Klebsiella pneumoniae 2.39 Klebsiella pneumoniae 2.34 D6 (+++) (A) * B 1189 (Standard) Klebsiella pneumoniae 2.04 Klebsiella pneumoniae 1.99 D7 (+++) (A) * B 1316 (Standard) Acinetobacter bereziniae 2.11 Acinetobacter bereziniae 1.91 Result overview table--continued on next page Report created at 2021-02-05T10:11:23 Research Use Only Page 1 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value D8 (+++) (A) * B 1290 (Standard) Klebsiella variicola 2.44 Klebsiella variicola 2.37 D9 (+++) (A) * C 2496 (Standard) Klebsiella pneumoniae 2.33 Klebsiella pneumoniae 2.28 D10 (+++) (A) * C 2547 (Standard) Klebsiella variicola 2.39 Klebsiella variicola 2.37 D11 (-) (C) * C 2621 (Standard) No Organism Identification Possible 1.60 No Organism Identification Possible 1.58 D12 (+++) (A) * C 2612 (Standard) Klebsiella pneumoniae 2.38 Klebsiella pneumoniae 2.35 E1 (+++) (A) * C 2656 (Standard) Klebsiella pneumoniae 2.05 Klebsiella pneumoniae 1.93 E2 (+++) (A) * C 2669 (Standard) Klebsiella pneumoniae 2.28 Klebsiella pneumoniae 2.25 E3 (+) (B) * C 2689 (Standard) Acinetobacter baumannii 1.96 Acinetobacter baumannii 1.94 E4 (+++) (A) * C 2681 (Standard) Klebsiella pneumoniae 2.12 Klebsiella pneumoniae 2.10 E5 (-) (C) XXX PRAZDNE (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-02-05T10:11:23 Research Use Only Page 2 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Matching Hints Matched Pattern Comment Acinetobacter baumannii 13101_1 CHB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii B389 UFL Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30007T HAM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30008 DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30011 DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii ECI RUH_875 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii ECII RUH_134 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii ECIII RUH_5875 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii GEIH_2000 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii LMG 994 HAM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter johnsonii LMG 983 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter johnsonii V773 MCRF Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter junii DSM 14968 HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter junii DSM 6964T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter tandoii DSM 14970T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter tjernbergiae DSM 14971T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-02-05T10:11:23 Research Use Only Page 3 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella aerogenes ATCC 13048T THL synonym of Enterobacter aerogenes Klebsiella aerogenes DSM 12058 DSM synonym of Enterobacter aerogenes Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_01 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Matching Hints table--continued on next page Report created at 2021-02-05T10:11:23 Research Use Only Page 4 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola CC_2123 MCW closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Report created at 2021-02-05T10:11:23 Research Use Only Page 5 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-02-05T10:11:23 Research Use Only Page 6 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 1 Sample Name: D1 Sample Description: Sample ID: * A 2386 Sample Creation Date/Time: 2021-02-05T09:52:33.685 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.23 574 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.20 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.20 573 4 (+++) Klebsiella variicola DSM 15968T DSM 2.13 244366 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.12 72407 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.09 574 7 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.07 72407 8 (+++) Klebsiella variicola DSM 15968T HAM 2.04 244366 9 (+) Klebsiella variicola 150519_03 SAH 1.96 244366 10 (+) Klebsiella variicola CC_2053 MCW 1.93 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 7 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 2 Sample Name: D2 Sample Description: Sample ID: * A 2387 Sample Creation Date/Time: 2021-02-05T09:52:33.685 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella variicola DSM 15968T HAM 1.83 244366 2 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.80 573 3 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.66 72407 4 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.62 574 5 (-) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.62 72407 6 (-) Klebsiella variicola NY_1064 MCW 1.56 244366 7 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.52 574 8 (-) Klebsiella variicola 37924 PFM 1.51 244366 9 (-) Klebsiella variicola DSM 15968T DSM 1.51 244366 10 (-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.40 574 Report created at 2021-02-05T10:11:23 Research Use Only Page 8 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 3 Sample Name: D3 Sample Description: Sample ID: * A 2389 Sample Creation Date/Time: 2021-02-05T09:52:33.686 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola 150519_03 SAH 2.33 244366 2 (+++) Klebsiella variicola CC_2053 MCW 2.33 244366 3 (+++) Klebsiella variicola 150416_01 BRUS 2.26 244366 4 (+++) Klebsiella variicola DSM 15968T DSM 2.24 244366 5 (+++) Klebsiella variicola NY_1064 MCW 2.22 244366 6 (+++) Klebsiella variicola TC_4131 MCW 2.21 244366 7 (+++) Klebsiella variicola 37924 PFM 2.21 244366 8 (+++) Klebsiella variicola 150519_01 SAH 2.19 244366 9 (+++) Klebsiella variicola NY_1125 MCW 2.17 244366 10 (+++) Klebsiella variicola CC_2123 MCW 2.16 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 9 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 4 Sample Name: D4 Sample Description: Sample ID: * A 2403-11 Sample Creation Date/Time: 2021-02-05T09:52:33.686 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.16 573 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.14 574 3 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.08 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.03 72407 5 (+++) Klebsiella variicola DSM 15968T HAM 2.01 244366 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.00 72407 7 (+) Klebsiella variicola DSM 15968T DSM 1.92 244366 8 (+) Klebsiella pneumoniae 37585 PFM 1.92 573 9 (+) Klebsiella variicola 150416_01 BRUS 1.91 244366 10 (+) Klebsiella variicola 37924 PFM 1.90 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 10 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 5 Sample Name: D5 Sample Description: Sample ID: * A 2403-12 Sample Creation Date/Time: 2021-02-05T09:52:33.687 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.39 72407 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.34 573 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.30 72407 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.27 574 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.26 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.24 72407 7 (+++) Klebsiella pneumoniae 37585 PFM 2.15 573 8 (+++) Klebsiella variicola CC_2053 MCW 2.08 244366 9 (+++) Klebsiella variicola 150416_01 BRUS 2.07 244366 10 (+++) Klebsiella variicola 150519_03 SAH 2.07 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 11 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 6 Sample Name: D6 Sample Description: Sample ID: * B 1189 Sample Creation Date/Time: 2021-02-05T09:52:33.687 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.04 573 2 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.99 574 3 (+) Klebsiella variicola DSM 15968T HAM 1.95 244366 4 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.93 574 5 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.89 72407 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.84 72407 7 (+) Klebsiella variicola NY_1064 MCW 1.79 244366 8 (+) Klebsiella variicola 37924 PFM 1.74 244366 9 (+) Klebsiella variicola DSM 15968T DSM 1.74 244366 10 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.73 72407 Report created at 2021-02-05T10:11:23 Research Use Only Page 12 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 7 Sample Name: D7 Sample Description: Sample ID: * B 1316 Sample Creation Date/Time: 2021-02-05T09:52:33.688 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Acinetobacter bereziniae CICC 23979_a CICC 2.11 469 2 (+) Acinetobacter bereziniae CIP 70_12T CIP 1.91 469 3 (+) Acinetobacter guillouiae CIP 63_46T CIP 1.81 469 4 (+) Acinetobacter guillouiae DSM 590T DSM 1.78 106649 5 (-) Acinetobacter johnsonii LMG 983 LMG 1.53 40214 6 (-) Acinetobacter tandoii DSM 14970T HAM 1.51 202954 7 (-) Acinetobacter junii DSM 6964T HAM 1.51 40215 8 (-) Acinetobacter tjernbergiae DSM 14971T HAM 1.49 202955 9 (-) Acinetobacter johnsonii V773 MCRF 1.44 40214 10 (-) Acinetobacter junii DSM 14968 HAM 1.41 40215 Report created at 2021-02-05T10:11:23 Research Use Only Page 13 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 8 Sample Name: D8 Sample Description: Sample ID: * B 1290 Sample Creation Date/Time: 2021-02-05T09:52:33.688 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola DSM 15968T DSM 2.44 244366 2 (+++) Klebsiella variicola CC_2053 MCW 2.37 244366 3 (+++) Klebsiella variicola 150416_01 BRUS 2.32 244366 4 (+++) Klebsiella variicola 150519_03 SAH 2.28 244366 5 (+++) Klebsiella variicola 37924 PFM 2.23 244366 6 (+++) Klebsiella variicola NY_1064 MCW 2.22 244366 7 (+++) Klebsiella variicola TC_4131 MCW 2.21 244366 8 (+++) Klebsiella variicola 150519_01 SAH 2.20 244366 9 (+++) Klebsiella variicola CC_2123 MCW 2.18 244366 10 (+++) Klebsiella variicola NY_1125 MCW 2.18 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 14 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 9 Sample Name: D9 Sample Description: Sample ID: * C 2496 Sample Creation Date/Time: 2021-02-05T09:52:33.689 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.33 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.28 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.28 573 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.21 574 5 (+++) Klebsiella pneumoniae 37585 PFM 2.16 573 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.12 574 7 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.08 72407 8 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.01 39831 9 (+++) Klebsiella pneumoniae 37595 PFM 2.00 573 10 (+++) Klebsiella variicola 150519_03 SAH 2.00 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 15 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 10 Sample Name: D10 Sample Description: Sample ID: * C 2547 Sample Creation Date/Time: 2021-02-05T09:52:33.689 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola CC_2053 MCW 2.39 244366 2 (+++) Klebsiella variicola DSM 15968T DSM 2.37 244366 3 (+++) Klebsiella variicola 150416_01 BRUS 2.31 244366 4 (+++) Klebsiella variicola 150519_03 SAH 2.29 244366 5 (+++) Klebsiella variicola NY_1125 MCW 2.27 244366 6 (+++) Klebsiella variicola TC_4131 MCW 2.25 244366 7 (+++) Klebsiella variicola NY_1064 MCW 2.25 244366 8 (+++) Klebsiella variicola CC_2123 MCW 2.25 244366 9 (+++) Klebsiella variicola 37924 PFM 2.21 244366 10 (+++) Klebsiella variicola 150519_01 SAH 2.20 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 16 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 11 Sample Name: D11 Sample Description: Sample ID: * C 2621 Sample Creation Date/Time: 2021-02-05T09:52:33.690 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) Klebsiella variicola DSM 15968T HAM 1.60 244366 2 (-) Klebsiella pneumoniae RV_BA_03_B LBK 1.58 573 3 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.51 574 4 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.51 574 5 (-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.50 574 6 (-) Klebsiella variicola DSM 15968T DSM 1.36 244366 7 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.34 72407 8 (-) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.33 72407 9 (-) Klebsiella aerogenes ATCC 13048T THL 1.26 28451 10 (-) Klebsiella variicola 150519_01 SAH 1.25 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 17 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 12 Sample Name: D12 Sample Description: Sample ID: * C 2612 Sample Creation Date/Time: 2021-02-05T09:52:33.690 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.38 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.35 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.28 574 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.23 72407 5 (+++) Klebsiella pneumoniae 37585 PFM 2.20 573 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.20 72407 7 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.17 574 8 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.11 39831 9 (+++) Klebsiella pneumoniae 37595 PFM 2.04 573 10 (+) Klebsiella variicola DSM 15968T HAM 1.98 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 18 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 13 Sample Name: E1 Sample Description: Sample ID: * C 2656 Sample Creation Date/Time: 2021-02-05T09:52:33.691 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.05 573 2 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.93 574 3 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.85 72407 4 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.82 574 5 (+) Klebsiella variicola DSM 15968T HAM 1.81 244366 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.79 72407 7 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.79 72407 8 (-) Klebsiella variicola NY_1125 MCW 1.68 244366 9 (-) Klebsiella variicola 37924 PFM 1.67 244366 10 (-) Klebsiella aerogenes DSM 12058 DSM 1.67 28451 Report created at 2021-02-05T10:11:23 Research Use Only Page 19 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 14 Sample Name: E2 Sample Description: Sample ID: * C 2669 Sample Creation Date/Time: 2021-02-05T09:52:33.691 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.28 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.25 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.24 574 4 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.24 72407 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.24 72407 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.22 574 7 (+++) Klebsiella pneumoniae 37585 PFM 2.11 573 8 (+++) Klebsiella variicola CC_2053 MCW 2.03 244366 9 (+) Klebsiella pneumoniae 37595 PFM 1.97 573 10 (+) Klebsiella variicola TC_4131 MCW 1.96 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 20 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 15 Sample Name: E3 Sample Description: Sample ID: * C 2689 Sample Creation Date/Time: 2021-02-05T09:52:33.692 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Acinetobacter baumannii GEIH_2000 HCB 1.96 470 2 (+) Acinetobacter baumannii 13101_1 CHB 1.94 470 3 (+) Acinetobacter baumannii LMG 994 HAM 1.93 470 4 (+) Acinetobacter baumannii ECIII RUH_5875 HCB 1.93 470 5 (+) Acinetobacter baumannii ECII RUH_134 HCB 1.89 470 6 (+) Acinetobacter baumannii DSM 30011 DSM 1.80 470 7 (+) Acinetobacter baumannii ECI RUH_875 HCB 1.78 470 8 (-) Acinetobacter baumannii DSM 30007T HAM 1.67 470 9 (-) Acinetobacter baumannii B389 UFL 1.65 470 10 (-) Acinetobacter baumannii DSM 30008 DSM 1.55 470 Report created at 2021-02-05T10:11:23 Research Use Only Page 21 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 16 Sample Name: E4 Sample Description: Sample ID: * C 2681 Sample Creation Date/Time: 2021-02-05T09:52:33.692 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.12 573 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.10 574 3 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.97 72407 4 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.95 72407 5 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.94 574 6 (+) Klebsiella variicola DSM 15968T HAM 1.94 244366 7 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.91 72407 8 (+) Klebsiella variicola 37924 PFM 1.87 244366 9 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.78 39831 10 (+) Klebsiella variicola DSM 15968T DSM 1.72 244366 Report created at 2021-02-05T10:11:23 Research Use Only Page 22 of 23 Run Identifier: 210205-0947-10184 Run Creation Date/Time: 2021-02-05T10:10:09.172 Sample 17 Sample Name: E5 Sample Description: Sample ID: XXX PRAZDNE Sample Creation Date/Time: 2021-02-05T09:52:33.693 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-02-05T10:11:23 Research Use Only Page 23 of 23