Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210212-1429-10199 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-02-12T14:42:52.741 Number of Tests: 22 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value F8 (+) (B) * A 2664 (Standard) Klebsiella variicola 1.89 Klebsiella pneumoniae 1.88 F9 (+++) (A) * A 2819 (Standard) Klebsiella pneumoniae 2.30 Klebsiella pneumoniae 2.28 F10 (+++) (A) * A 2852 (Standard) Klebsiella pneumoniae 2.13 Klebsiella pneumoniae 2.10 F11 (+++) (A) * B 2640 (Standard) Klebsiella variicola 2.34 Klebsiella variicola 2.32 F12 (+) (B) * B 2707 (Standard) Klebsiella pneumoniae 1.91 Klebsiella pneumoniae 1.86 G1 (+++) (A) * B 2737 (Standard) Klebsiella pneumoniae 2.18 Klebsiella pneumoniae 2.16 G2 (+++) (A) * B 2742 (Standard) Klebsiella pneumoniae 2.30 Klebsiella pneumoniae 2.27 Result overview table--continued on next page Report created at 2021-02-12T14:54:57 Research Use Only Page 1 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value G3 (+++) (A) * C 3004 (Standard) Klebsiella variicola 2.32 Klebsiella variicola 2.25 G4 (+++) (A) * C 3027 (Standard) Klebsiella pneumoniae 2.24 Klebsiella pneumoniae 2.23 G5 (+++) (A) * C 3035 (Standard) Klebsiella pneumoniae 2.02 Klebsiella pneumoniae 1.96 G6 (+++) (A) * C 3188 (Standard) Klebsiella pneumoniae 2.17 Klebsiella pneumoniae 1.95 G7 (+++) (A) * C 3190 (Standard) Klebsiella variicola 2.34 Klebsiella variicola 2.24 G8 (+++) (A) * C 3130 (Standard) Klebsiella variicola 2.18 Klebsiella variicola 2.16 G9 (-) (C) * 8 CRC DU BRU 1 (Standard) No Organism Identification Possible 1.64 No Organism Identification Possible 1.52 G10 (+++) (A) * 8 CRC DU GPA 1 (Standard) Candida dubliniensis 2.00 Candida dubliniensis 1.95 G11 (-) (C) * 8 CRC R GPA 1 (Standard) No Organism Identification Possible 1.45 No Organism Identification Possible 1.41 G12 (+) (B) * 7 CRC DU BRU 1 (Standard) Microbacterium oxydans 1.97 Microbacterium liquefaciens 1.89 H1 (+) (B) * 7 CRC DU BRU 2 (Standard) Tsukamurella tyrosinosolvens 1.71 No Organism Identification Possible 1.49 H2 (+++) (A) * 7 CRC DU GPA 1 (Standard) Streptococcus salivarius 2.12 Streptococcus salivarius 2.05 H3 (+) (B) * 7 CRC R GPA 1 (Standard) Geotrichum silvicola 1.88 Geotrichum silvicola 1.84 H4 (+) (B) * 7 CRC R GPA 2 (Standard) Candida inconspicua 1.85 No Organism Identification Possible 1.67 Result overview table--continued on next page Report created at 2021-02-12T14:54:57 Research Use Only Page 2 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value H5 (-) (C) XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-02-12T14:54:57 Research Use Only Page 3 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Matching Hints Matched Pattern Comment Corynebacterium mucifaciens CS 678_4 BRB Species mucifaciens / ureicelerivorans of the genus Corynebacterium have very similar patterns: Therefore distinguishing their species is difficult. Geotrichum candidum CBS 109_12 CBS Species candidum / silvicola of the genus Geotrichum have very similar patterns: Therefore distinguishing their species is difficult. Geotrichum candidum CBS 178_71T CBS Species candidum / silvicola of the genus Geotrichum have very similar patterns: Therefore distinguishing their species is difficult. Geotrichum candidum CBS 184_56 CBS Species candidum / silvicola of the genus Geotrichum have very similar patterns: Therefore distinguishing their species is difficult. Geotrichum candidum DSM 1240 DSM Species candidum / silvicola of the genus Geotrichum have very similar patterns: Therefore distinguishing their species is difficult. Geotrichum silvicola 04_007 LBK Species candidum / silvicola of the genus Geotrichum have very similar patterns: Therefore distinguishing their species is difficult. Geotrichum silvicola 04_188 LBK Species candidum / silvicola of the genus Geotrichum have very similar patterns: Therefore distinguishing their species is difficult. Geotrichum silvicola 04_322 LBK Species candidum / silvicola of the genus Geotrichum have very similar patterns: Therefore distinguishing their species is difficult. Geotrichum silvicola 2 LBK Species candidum / silvicola of the genus Geotrichum have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Matching Hints table--continued on next page Report created at 2021-02-12T14:54:57 Research Use Only Page 4 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_01 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola CC_2123 MCW closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Pseudomonas fulva LMG 11722T HAM is a member of Pseudomonas putida group Pseudomonas marginalis DSM 13124T HAM is a member of Pseudomonas fluorescens group Matching Hints table--continued on next page Report created at 2021-02-12T14:54:57 Research Use Only Page 5 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Matching Hints table--continued from previous page Matched Pattern Comment Pseudomonas mucidolens LMG 2223T HAM is a member of Pseudomonas fluorescens group Staphylococcus condimenti DSM 11675 DSM For the species carnosus / condimenti / piscifermentans of the genus Staphylococcus the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella paurometabola DSM 20162T DSM_2 For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella paurometabola DSM 46042 DSM For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella paurometabola DSM 46042 DSM_2 For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella paurometabola DSM 46065 DSM For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella paurometabola IMET 11082T HKJ For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella tyrosinosolvens DSM 44234T DSM For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella tyrosinosolvens DSM 44316 DSM For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella tyrosinosolvens DSM 44330 DSM For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Tsukamurella tyrosinosolvens DSM 44370 DSM For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Matching Hints table--continued on next page Report created at 2021-02-12T14:54:57 Research Use Only Page 6 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Matching Hints table--continued from previous page Matched Pattern Comment Tsukamurella tyrosinosolvens DSM 45557 DSM For the species carboxydivorans / inchonensis / paurometabola / pulmonis / strandjordii / tyrosinosolvens of the genus Tsukamurella the MALDI patterns and the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Report created at 2021-02-12T14:54:57 Research Use Only Page 7 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-02-12T14:54:57 Research Use Only Page 8 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 1 Sample Name: F8 Sample Description: Sample ID: * A 2664 Sample Creation Date/Time: 2021-02-12T14:35:03.021 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella variicola DSM 15968T HAM 1.89 244366 2 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.88 573 3 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.78 574 4 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.67 72407 5 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.67 574 6 (-) Klebsiella variicola DSM 15968T DSM 1.65 244366 7 (-) Klebsiella variicola 37924 PFM 1.62 244366 8 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.61 72407 9 (-) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.60 72407 10 (-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.57 574 Report created at 2021-02-12T14:54:57 Research Use Only Page 9 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 2 Sample Name: F9 Sample Description: Sample ID: * A 2819 Sample Creation Date/Time: 2021-02-12T14:35:03.022 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.30 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.28 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.24 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.21 72407 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.20 574 6 (+++) Klebsiella pneumoniae 37585 PFM 2.12 573 7 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.10 39831 8 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.05 574 9 (+) Klebsiella pneumoniae 37595 PFM 1.97 573 10 (+) Klebsiella variicola DSM 15968T HAM 1.96 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 10 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 3 Sample Name: F10 Sample Description: Sample ID: * A 2852 Sample Creation Date/Time: 2021-02-12T14:35:03.023 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.13 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.10 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.08 72407 4 (+++) Klebsiella pneumoniae 37585 PFM 2.04 573 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.03 574 6 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.01 573 7 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.92 39831 8 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.89 574 9 (+) Klebsiella variicola TC_4131 MCW 1.82 244366 10 (+) Klebsiella variicola DSM 15968T HAM 1.81 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 11 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 4 Sample Name: F11 Sample Description: Sample ID: * B 2640 Sample Creation Date/Time: 2021-02-12T14:35:03.024 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola DSM 15968T DSM 2.34 244366 2 (+++) Klebsiella variicola CC_2053 MCW 2.32 244366 3 (+++) Klebsiella variicola 150416_01 BRUS 2.26 244366 4 (+++) Klebsiella variicola 37924 PFM 2.24 244366 5 (+++) Klebsiella variicola NY_1125 MCW 2.24 244366 6 (+++) Klebsiella variicola TC_4131 MCW 2.23 244366 7 (+++) Klebsiella variicola 150519_03 SAH 2.20 244366 8 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.18 573 9 (+++) Klebsiella variicola CC_2123 MCW 2.18 244366 10 (+++) Klebsiella variicola NY_1064 MCW 2.14 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 12 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 5 Sample Name: F12 Sample Description: Sample ID: * B 2707 Sample Creation Date/Time: 2021-02-12T14:35:03.024 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.91 573 2 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.86 574 3 (+) Klebsiella variicola DSM 15968T HAM 1.85 244366 4 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.83 72407 5 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.70 72407 6 (-) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.67 39831 7 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.66 574 8 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.61 72407 9 (-) Klebsiella pneumoniae 37585 PFM 1.56 573 10 (-) Klebsiella variicola 37924 PFM 1.49 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 13 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 6 Sample Name: G1 Sample Description: Sample ID: * B 2737 Sample Creation Date/Time: 2021-02-12T14:35:03.025 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.18 573 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.16 574 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.11 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.04 72407 5 (+++) Klebsiella variicola DSM 15968T HAM 2.03 244366 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.00 72407 7 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.98 574 8 (+) Klebsiella variicola DSM 15968T DSM 1.96 244366 9 (+) Klebsiella pneumoniae 37585 PFM 1.96 573 10 (+) Klebsiella variicola NY_1064 MCW 1.93 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 14 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 7 Sample Name: G2 Sample Description: Sample ID: * B 2742 Sample Creation Date/Time: 2021-02-12T14:35:03.026 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.30 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.27 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.22 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.17 72407 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.17 574 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.13 574 7 (+++) Klebsiella pneumoniae 37585 PFM 2.06 573 8 (+) Klebsiella pneumoniae 37595 PFM 1.97 573 9 (+) Klebsiella variicola DSM 15968T HAM 1.94 244366 10 (+) Klebsiella variicola DSM 15968T DSM 1.88 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 15 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 8 Sample Name: G3 Sample Description: Sample ID: * C 3004 Sample Creation Date/Time: 2021-02-12T14:35:03.026 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola DSM 15968T DSM 2.32 244366 2 (+++) Klebsiella variicola CC_2053 MCW 2.25 244366 3 (+++) Klebsiella variicola 150416_01 BRUS 2.19 244366 4 (+++) Klebsiella variicola 150519_03 SAH 2.18 244366 5 (+++) Klebsiella variicola TC_4131 MCW 2.15 244366 6 (+++) Klebsiella variicola NY_1064 MCW 2.13 244366 7 (+++) Klebsiella variicola NY_1125 MCW 2.08 244366 8 (+++) Klebsiella variicola 150519_01 SAH 2.07 244366 9 (+++) Klebsiella variicola 37924 PFM 2.04 244366 10 (+++) Klebsiella variicola CC_2123 MCW 2.03 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 16 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 9 Sample Name: G4 Sample Description: Sample ID: * C 3027 Sample Creation Date/Time: 2021-02-12T14:35:03.027 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.24 72407 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.23 574 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.22 573 4 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.21 72407 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.12 72407 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.12 574 7 (+++) Klebsiella pneumoniae 37585 PFM 2.11 573 8 (+++) Klebsiella variicola 150416_01 BRUS 2.03 244366 9 (+++) Klebsiella pneumoniae 37595 PFM 2.02 573 10 (+++) Klebsiella variicola NY_1064 MCW 2.01 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 17 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 10 Sample Name: G5 Sample Description: Sample ID: * C 3035 Sample Creation Date/Time: 2021-02-12T14:35:03.028 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.02 574 2 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.96 573 3 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.94 72407 4 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.93 72407 5 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.86 574 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.85 72407 7 (+) Klebsiella variicola DSM 15968T HAM 1.84 244366 8 (+) Klebsiella pneumoniae 37595 PFM 1.83 573 9 (+) Klebsiella pneumoniae 37585 PFM 1.82 573 10 (-) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.67 39831 Report created at 2021-02-12T14:54:57 Research Use Only Page 18 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 11 Sample Name: G6 Sample Description: Sample ID: * C 3188 Sample Creation Date/Time: 2021-02-12T14:35:03.029 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.17 72407 2 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.95 72407 3 (+) Klebsiella pneumoniae 37585 PFM 1.92 573 4 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.91 574 5 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.88 573 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.87 72407 7 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.83 574 8 (+) Klebsiella pneumoniae 37595 PFM 1.77 573 9 (-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.67 574 10 (-) Klebsiella variicola TC_4131 MCW 1.67 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 19 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 12 Sample Name: G7 Sample Description: Sample ID: * C 3190 Sample Creation Date/Time: 2021-02-12T14:35:03.029 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola DSM 15968T DSM 2.34 244366 2 (+++) Klebsiella variicola CC_2053 MCW 2.24 244366 3 (+++) Klebsiella variicola 150416_01 BRUS 2.22 244366 4 (+++) Klebsiella variicola 150519_03 SAH 2.17 244366 5 (+++) Klebsiella variicola NY_1125 MCW 2.14 244366 6 (+++) Klebsiella variicola TC_4131 MCW 2.12 244366 7 (+++) Klebsiella variicola CC_2123 MCW 2.11 244366 8 (+++) Klebsiella variicola NY_1064 MCW 2.04 244366 9 (+++) Klebsiella variicola 37924 PFM 2.04 244366 10 (+) Klebsiella variicola DSM 15968T HAM 1.99 244366 Report created at 2021-02-12T14:54:57 Research Use Only Page 20 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 13 Sample Name: G8 Sample Description: Sample ID: * C 3130 Sample Creation Date/Time: 2021-02-12T14:35:03.030 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola 150416_01 BRUS 2.18 244366 2 (+++) Klebsiella variicola TC_4131 MCW 2.16 244366 3 (+++) Klebsiella variicola NY_1064 MCW 2.12 244366 4 (+++) Klebsiella variicola CC_2053 MCW 2.11 244366 5 (+++) Klebsiella variicola 37924 PFM 2.01 244366 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.00 574 7 (+) Klebsiella variicola CC_2123 MCW 1.97 244366 8 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.96 72407 9 (+) Klebsiella variicola NY_1125 MCW 1.95 244366 10 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.94 72407 Report created at 2021-02-12T14:54:57 Research Use Only Page 21 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 14 Sample Name: G9 Sample Description: Sample ID: * 8 CRC DU BRU 1 Sample Creation Date/Time: 2021-02-12T14:35:03.031 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) Candida dubliniensis Eg 202 CBS 1.64 42374 2 (-) Candida dubliniensis SA 108 CBS 1.52 42374 3 (-) Candida dubliniensis CD 524 CBS 1.50 42374 4 (-) Candida dubliniensis CBS 7988 CBS 1.50 42374 5 (-) Candida dubliniensis 99 PSB 1.45 42374 6 (-) Candida dubliniensis CD 6024 CBS 1.38 42374 7 (-) Candida dubliniensis 41_3 ZZMK 1.37 42374 8 (-) Candida albicans DSM 3454 DSM 1.34 5476 9 (-) Candida dubliniensis 20 UKE 1.32 42374 10 (-) Candida dubliniensis CBS 8501 CBS 1.31 42374 Report created at 2021-02-12T14:54:57 Research Use Only Page 22 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 15 Sample Name: G10 Sample Description: Sample ID: * 8 CRC DU GPA 1 Sample Creation Date/Time: 2021-02-12T14:35:03.032 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Candida dubliniensis 41_3 ZZMK 2.00 42374 2 (+) Candida dubliniensis CBS 7988 CBS 1.95 42374 3 (+) Candida dubliniensis 41_9 ZZMK 1.85 42374 4 (+) Candida dubliniensis CD 524 CBS 1.81 42374 5 (+) Candida dubliniensis 99 PSB 1.75 42374 6 (+) Candida dubliniensis 20 UKE 1.74 42374 7 (-) Candida dubliniensis Eg 202 CBS 1.64 42374 8 (-) Candida dubliniensis SA 108 CBS 1.62 42374 9 (-) Candida dubliniensis 41_5 ZZMK 1.61 42374 10 (-) Candida dubliniensis SA 121 CBS 1.48 42374 Report created at 2021-02-12T14:54:57 Research Use Only Page 23 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 16 Sample Name: G11 Sample Description: Sample ID: * 8 CRC R GPA 1 Sample Creation Date/Time: 2021-02-12T14:35:03.033 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) Candida dubliniensis SA 121 CBS 1.45 42374 2 (-) Candida dubliniensis 99 PSB 1.41 42374 3 (-) Candida dubliniensis CBS 7987 CBS 1.36 42374 4 (-) Staphylococcus condimenti DSM 11675 DSM 1.30 70255 5 (-) Paraclostridium bifermentans 2273_CCUG 35297 BOG 1.30 1490 6 (-) Staphylococcus pasteuri DSM 10657 DSM 1.29 45972 7 (-) Candida dubliniensis CD 524 CBS 1.29 42374 8 (-) Staphylococcus sciuri ssp sciuri DSM 6671 DSM 1.28 147467 9 (-) Pseudomonas marginalis DSM 13124T HAM 1.26 298 10 (-) Corynebacterium confusum DSM 44384T DSM 1.24 71254 Report created at 2021-02-12T14:54:57 Research Use Only Page 24 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 17 Sample Name: G12 Sample Description: Sample ID: * 7 CRC DU BRU 1 Sample Creation Date/Time: 2021-02-12T14:35:03.034 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Microbacterium oxydans DSM 20578T DSM 1.97 82380 2 (+) Microbacterium liquefaciens HKI 11374 HKJ 1.89 33918 3 (+) Microbacterium maritypicum DSM 12512T DSM 1.81 300020 4 (-) Microbacterium liquefaciens DSM 20637 DSM 1.67 33918 5 (-) Microbacterium liquefaciens DSM 20638T DSM 1.60 33918 6 (-) Microbacterium saperdae IMET 11076T HKJ 1.32 69368 7 (-) Corynebacterium mucifaciens CS 678_4 BRB 1.29 57171 8 (-) Pseudomonas taetrolens LMG 2336T HAM 1.28 47884 9 (-) Microbacterium hydrocarbonoxydans DSM 16089T DSM 1.28 273678 10 (-) Pseudomonas mucidolens LMG 2223T HAM 1.23 46679 Report created at 2021-02-12T14:54:57 Research Use Only Page 25 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 18 Sample Name: H1 Sample Description: Sample ID: * 7 CRC DU BRU 2 Sample Creation Date/Time: 2021-02-12T14:35:03.034 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Tsukamurella tyrosinosolvens DSM 45557 DSM 1.71 57704 2 (-) Tsukamurella paurometabola DSM 46065 DSM 1.49 2061 3 (-) Tsukamurella paurometabola DSM 46042 DSM_2 1.49 2061 4 (-) Tsukamurella tyrosinosolvens DSM 44330 DSM 1.35 57704 5 (-) Tsukamurella tyrosinosolvens DSM 44234T DSM 1.33 57704 6 (-) Tsukamurella tyrosinosolvens DSM 44370 DSM 1.32 57704 7 (-) Tsukamurella tyrosinosolvens DSM 44316 DSM 1.28 57704 8 (-) Tsukamurella paurometabola IMET 11082T HKJ 1.24 2061 9 (-) Tsukamurella paurometabola DSM 46042 DSM 1.20 2061 10 (-) Tsukamurella paurometabola DSM 20162T DSM_2 1.19 2061 Report created at 2021-02-12T14:54:57 Research Use Only Page 26 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 19 Sample Name: H2 Sample Description: Sample ID: * 7 CRC DU GPA 1 Sample Creation Date/Time: 2021-02-12T14:35:03.035 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Streptococcus salivarius ssp salivarius 140417_01 ETL 2.12 1304 2 (+++) Streptococcus salivarius DSM 20560T DSM 2.05 1304 3 (+++) Streptococcus salivarius DSM 20560T BRB 2.01 1304 4 (+++) Streptococcus vestibularis LMG 14645 LMG 2.00 1343 5 (+) Streptococcus vestibularis CCUG 51352 CCUG 1.98 1343 6 (+) Streptococcus vestibularis 14147704_2 MVD 1.96 1343 7 (+) Streptococcus vestibularis DSM 5636T DSM_2 1.93 1343 8 (+) Streptococcus vestibularis CCUG 61229 CCUG 1.91 1343 9 (+) Streptococcus salivarius 0807M25049501 IBS 1.86 1304 10 (+) Streptococcus vestibularis 1C15011954_3 MVD 1.80 1343 Report created at 2021-02-12T14:54:57 Research Use Only Page 27 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 20 Sample Name: H3 Sample Description: Sample ID: * 7 CRC R GPA 1 Sample Creation Date/Time: 2021-02-12T14:35:03.036 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Geotrichum silvicola 04_188 LBK 1.88 215419 2 (+) Geotrichum silvicola 04_322 LBK 1.84 215419 3 (+) Geotrichum silvicola 04_007 LBK 1.77 215419 4 (+) Geotrichum candidum CBS 184_56 CBS 1.74 27317 5 (-) Geotrichum candidum CBS 178_71T CBS 1.66 27317 6 (-) Geotrichum candidum DSM 1240 DSM 1.47 27317 7 (-) Geotrichum candidum CBS 109_12 CBS 1.44 27317 8 (-) Geotrichum sp 116 PSB 1.40 43987 9 (-) Geotrichum silvicola 2 LBK 1.38 215419 10 (-) Pseudomonas fulva LMG 11722T HAM 1.36 47880 Report created at 2021-02-12T14:54:57 Research Use Only Page 28 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 21 Sample Name: H4 Sample Description: Sample ID: * 7 CRC R GPA 2 Sample Creation Date/Time: 2021-02-12T14:35:03.037 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Candida inconspicua CBS 2833 CBS 1.85 52247 2 (-) Candida inconspicua CBS 180T CBS 1.67 52247 3 (-) Brevibacterium casei 12017785 MVD 1.43 33889 4 (-) Candida inconspicua CBS 990 CBS 1.40 52247 5 (-) Candida lambica CBS 603 CBS 1.40 53655 6 (-) Paeniglutamicibacter gangotriensis DSM 15796T DSM 1.29 254787 7 (-) Pichia norvegensis 98 PSB 1.27 4921 8 (-) Clostridium carnis DSM 1293T DSM 1.25 1530 9 (-) Alcaligenes faecalis ssp parafaecalis DSM 13975T HAM 1.22 171260 10 (-) Kocuria marina CICC 23948 CICC 1.21 223184 Report created at 2021-02-12T14:54:57 Research Use Only Page 29 of 30 Run Identifier: 210212-1429-10199 Run Creation Date/Time: 2021-02-12T14:42:52.741 Sample 22 Sample Name: H5 Sample Description: Sample ID: XXX Sample Creation Date/Time: 2021-02-12T14:35:03.038 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-02-12T14:54:57 Research Use Only Page 30 of 30