Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210218-0909-10199 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-02-18T09:28:30.305 Number of Tests: 22 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value A1 (+++) (A) * A 3067 (Standard) Klebsiella pneumoniae 2.28 Klebsiella pneumoniae 2.24 A2 (+++) (A) * A 3136 (Standard) Klebsiella variicola 2.32 Klebsiella variicola 2.20 A3 (+++) (A) * A 3141 (Standard) Klebsiella variicola 2.35 Klebsiella variicola 2.26 A4 (+++) (A) * A 3147 (Standard) Klebsiella pneumoniae 2.42 Klebsiella pneumoniae 2.40 A5 (+++) (A) * A 3196 (Standard) Acinetobacter baumannii 2.32 Acinetobacter baumannii 2.28 A6 (+++) (A) * A 3227 (Standard) Klebsiella pneumoniae 2.36 Klebsiella pneumoniae 2.32 A7 (+++) (A) * A 3229 (Standard) Acinetobacter johnsonii 2.00 Acinetobacter johnsonii 1.95 Result overview table--continued on next page Report created at 2021-02-18T09:31:26 Research Use Only Page 1 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value A8 (+++) (A) * A 3283 (Standard) Klebsiella variicola 2.40 Klebsiella variicola 2.39 A9 (+++) (A) * B 3023 (Standard) Klebsiella pneumoniae 2.23 Klebsiella pneumoniae 2.21 A10 (+++) (A) * B 3082 (Standard) Klebsiella pneumoniae 2.17 Klebsiella pneumoniae 2.16 A11 (+++) (A) * B 3083 (Standard) Klebsiella pneumoniae 2.02 Klebsiella pneumoniae 1.97 A12 (+++) (A) * B 3161 (Standard) Klebsiella variicola 2.38 Klebsiella variicola 2.36 B1 (+++) (A) * C 3715 (Standard) Klebsiella variicola 2.44 Klebsiella variicola 2.38 B2 (+++) (A) * C 3716 (Standard) Klebsiella pneumoniae 2.25 Klebsiella pneumoniae 2.21 B3 (+++) (A) * C 3721 (Standard) Klebsiella pneumoniae 2.31 Klebsiella pneumoniae 2.29 B4 (+++) (A) * C 3740 (Standard) Klebsiella pneumoniae 2.26 Klebsiella pneumoniae 2.10 B5 (+++) (A) * C 3589 (Standard) Klebsiella pneumoniae 2.28 Klebsiella pneumoniae 2.23 B6 (+++) (A) * C 3596 (Standard) Klebsiella pneumoniae 2.18 Klebsiella pneumoniae 2.15 B7 (+++) (A) * C 3603 (Standard) Klebsiella pneumoniae 2.34 Klebsiella pneumoniae 2.31 B8 (+++) (A) * C 3703 (Standard) Klebsiella pneumoniae 2.24 Klebsiella pneumoniae 2.23 B9 (+++) (A) * C 3714 (Standard) Klebsiella pneumoniae 2.35 Klebsiella pneumoniae 2.27 B10 (-) (C) XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-02-18T09:31:26 Research Use Only Page 2 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Matching Hints Matched Pattern Comment Acinetobacter baumannii 13101_1 CHB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii B389 UFL Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30007T HAM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30008 DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30011 DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii ECI RUH_875 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii ECII RUH_134 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii ECIII RUH_5875 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii GEIH_2000 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii LMG 994 HAM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter johnsonii 10036669_102 USH Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter johnsonii 2_1 TUB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter johnsonii 31 PIM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter johnsonii DSM 6963T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter johnsonii DSM 6963T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter johnsonii LMG 1005 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-02-18T09:31:26 Research Use Only Page 3 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Matching Hints table--continued from previous page Matched Pattern Comment Acinetobacter johnsonii LMG 1326 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter johnsonii LMG 983 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter johnsonii V773 MCRF Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Acinetobacter tandoii DSM 14970T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_01 SAH closely related to Klebsiella pneumoniae Matching Hints table--continued on next page Report created at 2021-02-18T09:31:26 Research Use Only Page 4 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola CC_2123 MCW closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Report created at 2021-02-18T09:31:26 Research Use Only Page 5 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-02-18T09:31:26 Research Use Only Page 6 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 1 Sample Name: A1 Sample Description: Sample ID: * A 3067 Sample Creation Date/Time: 2021-02-18T09:17:18.299 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.28 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.24 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.23 573 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.17 574 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.15 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.10 72407 7 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.01 39831 8 (+) Klebsiella pneumoniae 37595 PFM 1.95 573 9 (+) Klebsiella variicola DSM 15968T HAM 1.92 244366 10 (+) Klebsiella variicola 150519_03 SAH 1.91 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 7 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 2 Sample Name: A2 Sample Description: Sample ID: * A 3136 Sample Creation Date/Time: 2021-02-18T09:17:18.299 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola DSM 15968T DSM 2.32 244366 2 (+++) Klebsiella variicola 150416_01 BRUS 2.20 244366 3 (+++) Klebsiella variicola NY_1125 MCW 2.19 244366 4 (+++) Klebsiella variicola CC_2053 MCW 2.18 244366 5 (+++) Klebsiella variicola TC_4131 MCW 2.16 244366 6 (+++) Klebsiella variicola 150519_03 SAH 2.16 244366 7 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.12 72407 8 (+++) Klebsiella variicola DSM 15968T HAM 2.12 244366 9 (+++) Klebsiella variicola CC_2123 MCW 2.08 244366 10 (+++) Klebsiella variicola NY_1064 MCW 2.07 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 8 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 3 Sample Name: A3 Sample Description: Sample ID: * A 3141 Sample Creation Date/Time: 2021-02-18T09:17:18.300 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola DSM 15968T DSM 2.35 244366 2 (+++) Klebsiella variicola CC_2053 MCW 2.26 244366 3 (+++) Klebsiella variicola TC_4131 MCW 2.26 244366 4 (+++) Klebsiella variicola DSM 15968T HAM 2.23 244366 5 (+++) Klebsiella variicola 150519_03 SAH 2.23 244366 6 (+++) Klebsiella variicola 150416_01 BRUS 2.22 244366 7 (+++) Klebsiella variicola 37924 PFM 2.22 244366 8 (+++) Klebsiella variicola NY_1125 MCW 2.19 244366 9 (+++) Klebsiella variicola NY_1064 MCW 2.15 244366 10 (+++) Klebsiella variicola CC_2123 MCW 2.13 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 9 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 4 Sample Name: A4 Sample Description: Sample ID: * A 3147 Sample Creation Date/Time: 2021-02-18T09:17:18.300 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.42 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.40 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.31 574 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.31 72407 5 (+++) Klebsiella pneumoniae 37585 PFM 2.29 573 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.24 574 7 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.24 72407 8 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.22 39831 9 (+++) Klebsiella variicola 150519_03 SAH 2.21 244366 10 (+++) Klebsiella variicola 150416_01 BRUS 2.18 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 10 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 5 Sample Name: A5 Sample Description: Sample ID: * A 3196 Sample Creation Date/Time: 2021-02-18T09:17:18.301 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Acinetobacter baumannii LMG 994 HAM 2.32 470 2 (+++) Acinetobacter baumannii ECII RUH_134 HCB 2.28 470 3 (+++) Acinetobacter baumannii GEIH_2000 HCB 2.28 470 4 (+++) Acinetobacter baumannii ECIII RUH_5875 HCB 2.26 470 5 (+++) Acinetobacter baumannii ECI RUH_875 HCB 2.19 470 6 (+++) Acinetobacter baumannii DSM 30011 DSM 2.13 470 7 (+++) Acinetobacter baumannii 13101_1 CHB 2.10 470 8 (+++) Acinetobacter baumannii DSM 30007T HAM 2.06 470 9 (+++) Acinetobacter baumannii DSM 30008 DSM 2.05 470 10 (+) Acinetobacter baumannii B389 UFL 1.81 470 Report created at 2021-02-18T09:31:26 Research Use Only Page 11 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 6 Sample Name: A6 Sample Description: Sample ID: * A 3227 Sample Creation Date/Time: 2021-02-18T09:17:18.301 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.36 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.32 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.25 72407 4 (+++) Klebsiella pneumoniae 37585 PFM 2.24 573 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.23 574 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.20 574 7 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.18 573 8 (+++) Klebsiella variicola CC_2053 MCW 2.08 244366 9 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.06 39831 10 (+++) Klebsiella pneumoniae 37595 PFM 2.05 573 Report created at 2021-02-18T09:31:26 Research Use Only Page 12 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 7 Sample Name: A7 Sample Description: Sample ID: * A 3229 Sample Creation Date/Time: 2021-02-18T09:17:18.302 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Acinetobacter johnsonii LMG 983 LMG 2.00 40214 2 (+) Acinetobacter johnsonii V773 MCRF 1.95 40214 3 (+) Acinetobacter johnsonii DSM 6963T HAM 1.91 40214 4 (+) Acinetobacter johnsonii 31 PIM 1.91 40214 5 (+) Acinetobacter johnsonii 2_1 TUB 1.90 40214 6 (+) Acinetobacter johnsonii 10036669_102 USH 1.88 40214 7 (+) Acinetobacter johnsonii LMG 1326 LMG 1.84 40214 8 (+) Acinetobacter johnsonii LMG 1005 LMG 1.78 40214 9 (+) Acinetobacter johnsonii DSM 6963T DSM 1.77 40214 10 (-) Acinetobacter tandoii DSM 14970T HAM 1.36 202954 Report created at 2021-02-18T09:31:26 Research Use Only Page 13 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 8 Sample Name: A8 Sample Description: Sample ID: * A 3283 Sample Creation Date/Time: 2021-02-18T09:17:18.302 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola DSM 15968T DSM 2.40 244366 2 (+++) Klebsiella variicola CC_2053 MCW 2.39 244366 3 (+++) Klebsiella variicola 150519_03 SAH 2.31 244366 4 (+++) Klebsiella variicola 150416_01 BRUS 2.30 244366 5 (+++) Klebsiella variicola TC_4131 MCW 2.29 244366 6 (+++) Klebsiella variicola NY_1125 MCW 2.26 244366 7 (+++) Klebsiella variicola 37924 PFM 2.26 244366 8 (+++) Klebsiella variicola NY_1064 MCW 2.22 244366 9 (+++) Klebsiella variicola CC_2123 MCW 2.17 244366 10 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.17 72407 Report created at 2021-02-18T09:31:26 Research Use Only Page 14 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 9 Sample Name: A9 Sample Description: Sample ID: * B 3023 Sample Creation Date/Time: 2021-02-18T09:17:18.303 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.23 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.21 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.16 72407 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.13 574 5 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.13 573 6 (+++) Klebsiella variicola 150416_01 BRUS 2.07 244366 7 (+++) Klebsiella pneumoniae 37585 PFM 2.06 573 8 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.06 574 9 (+++) Klebsiella variicola CC_2053 MCW 2.05 244366 10 (+++) Klebsiella variicola DSM 15968T DSM 2.00 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 15 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 10 Sample Name: A10 Sample Description: Sample ID: * B 3082 Sample Creation Date/Time: 2021-02-18T09:17:18.303 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.17 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.16 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.13 574 4 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.12 72407 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.11 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.02 72407 7 (+++) Klebsiella variicola DSM 15968T HAM 2.02 244366 8 (+) Klebsiella pneumoniae 37585 PFM 1.99 573 9 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.88 39831 10 (+) Klebsiella variicola DSM 15968T DSM 1.86 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 16 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 11 Sample Name: A11 Sample Description: Sample ID: * B 3083 Sample Creation Date/Time: 2021-02-18T09:17:18.304 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.02 574 2 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.97 573 3 (+) Klebsiella variicola DSM 15968T HAM 1.90 244366 4 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.90 72407 5 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.81 574 6 (+) Klebsiella variicola 37924 PFM 1.75 244366 7 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.74 72407 8 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.72 72407 9 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.71 39831 10 (+) Klebsiella variicola DSM 15968T DSM 1.70 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 17 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 12 Sample Name: A12 Sample Description: Sample ID: * B 3161 Sample Creation Date/Time: 2021-02-18T09:17:18.304 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola CC_2053 MCW 2.38 244366 2 (+++) Klebsiella variicola DSM 15968T DSM 2.36 244366 3 (+++) Klebsiella variicola 150416_01 BRUS 2.34 244366 4 (+++) Klebsiella variicola TC_4131 MCW 2.34 244366 5 (+++) Klebsiella variicola NY_1125 MCW 2.32 244366 6 (+++) Klebsiella variicola CC_2123 MCW 2.30 244366 7 (+++) Klebsiella variicola NY_1064 MCW 2.30 244366 8 (+++) Klebsiella variicola 150519_03 SAH 2.29 244366 9 (+++) Klebsiella variicola 37924 PFM 2.24 244366 10 (+++) Klebsiella variicola 150519_01 SAH 2.16 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 18 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 13 Sample Name: B1 Sample Description: Sample ID: * C 3715 Sample Creation Date/Time: 2021-02-18T09:17:18.305 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola CC_2053 MCW 2.44 244366 2 (+++) Klebsiella variicola NY_1064 MCW 2.38 244366 3 (+++) Klebsiella variicola 150519_03 SAH 2.37 244366 4 (+++) Klebsiella variicola TC_4131 MCW 2.36 244366 5 (+++) Klebsiella variicola 150416_01 BRUS 2.35 244366 6 (+++) Klebsiella variicola 150519_01 SAH 2.31 244366 7 (+++) Klebsiella variicola CC_2123 MCW 2.28 244366 8 (+++) Klebsiella variicola 37924 PFM 2.27 244366 9 (+++) Klebsiella variicola DSM 15968T DSM 2.21 244366 10 (+++) Klebsiella variicola NY_1125 MCW 2.20 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 19 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 14 Sample Name: B2 Sample Description: Sample ID: * C 3716 Sample Creation Date/Time: 2021-02-18T09:17:18.305 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.25 573 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.21 574 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.11 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.05 72407 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.04 574 6 (+++) Klebsiella variicola DSM 15968T HAM 2.02 244366 7 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.01 39831 8 (+) Klebsiella variicola DSM 15968T DSM 1.95 244366 9 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.93 72407 10 (+) Klebsiella variicola NY_1064 MCW 1.90 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 20 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 15 Sample Name: B3 Sample Description: Sample ID: * C 3721 Sample Creation Date/Time: 2021-02-18T09:17:18.306 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.31 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.29 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.28 574 4 (+++) Klebsiella pneumoniae 37585 PFM 2.21 573 5 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.17 573 6 (+++) Klebsiella variicola 150416_01 BRUS 2.17 244366 7 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.17 574 8 (+++) Klebsiella variicola 150519_03 SAH 2.06 244366 9 (+++) Klebsiella pneumoniae 37595 PFM 2.04 573 10 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.03 72407 Report created at 2021-02-18T09:31:26 Research Use Only Page 21 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 16 Sample Name: B4 Sample Description: Sample ID: * C 3740 Sample Creation Date/Time: 2021-02-18T09:17:18.307 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.26 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.10 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.10 574 4 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.05 573 5 (+++) Klebsiella pneumoniae 37585 PFM 2.03 573 6 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.98 574 7 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.95 72407 8 (+) Klebsiella pneumoniae 37595 PFM 1.93 573 9 (+) Klebsiella variicola 150519_03 SAH 1.91 244366 10 (+) Klebsiella variicola 150416_01 BRUS 1.86 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 22 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 17 Sample Name: B5 Sample Description: Sample ID: * C 3589 Sample Creation Date/Time: 2021-02-18T09:17:18.307 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.28 72407 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.23 574 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.22 573 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.18 72407 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.15 72407 6 (+++) Klebsiella pneumoniae 37585 PFM 2.15 573 7 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.09 574 8 (+++) Klebsiella variicola 150416_01 BRUS 2.00 244366 9 (+++) Klebsiella pneumoniae 37595 PFM 2.00 573 10 (+) Klebsiella variicola 150519_03 SAH 1.98 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 23 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 18 Sample Name: B6 Sample Description: Sample ID: * C 3596 Sample Creation Date/Time: 2021-02-18T09:17:18.308 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.18 574 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.15 573 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.14 574 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.05 72407 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.01 72407 6 (+++) Klebsiella variicola DSM 15968T HAM 2.00 244366 7 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.99 72407 8 (+) Klebsiella variicola NY_1064 MCW 1.94 244366 9 (+) Klebsiella variicola 150519_03 SAH 1.94 244366 10 (+) Klebsiella variicola 37924 PFM 1.93 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 24 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 19 Sample Name: B7 Sample Description: Sample ID: * C 3603 Sample Creation Date/Time: 2021-02-18T09:17:18.308 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.34 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.31 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.24 573 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.23 574 5 (+++) Klebsiella pneumoniae 37585 PFM 2.19 573 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.19 574 7 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.11 72407 8 (+++) Klebsiella variicola 150416_01 BRUS 2.10 244366 9 (+++) Klebsiella variicola 150519_03 SAH 2.04 244366 10 (+++) Klebsiella pneumoniae 37595 PFM 2.00 573 Report created at 2021-02-18T09:31:26 Research Use Only Page 25 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 20 Sample Name: B8 Sample Description: Sample ID: * C 3703 Sample Creation Date/Time: 2021-02-18T09:17:18.309 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.24 573 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.23 574 3 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.22 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.21 72407 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.16 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.14 72407 7 (+) Klebsiella pneumoniae 37585 PFM 1.96 573 8 (+) Klebsiella variicola DSM 15968T HAM 1.96 244366 9 (+) Klebsiella variicola DSM 15968T DSM 1.93 244366 10 (+) Klebsiella pneumoniae 37595 PFM 1.92 573 Report created at 2021-02-18T09:31:26 Research Use Only Page 26 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 21 Sample Name: B9 Sample Description: Sample ID: * C 3714 Sample Creation Date/Time: 2021-02-18T09:17:18.309 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.35 574 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.27 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.24 72407 4 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.18 573 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.17 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.16 72407 7 (+++) Klebsiella pneumoniae 37585 PFM 2.14 573 8 (+++) Klebsiella variicola 150416_01 BRUS 2.06 244366 9 (+++) Klebsiella variicola 37924 PFM 2.01 244366 10 (+) Klebsiella variicola TC_4131 MCW 1.99 244366 Report created at 2021-02-18T09:31:26 Research Use Only Page 27 of 28 Run Identifier: 210218-0909-10199 Run Creation Date/Time: 2021-02-18T09:28:30.305 Sample 22 Sample Name: B10 Sample Description: Sample ID: XXX Sample Creation Date/Time: 2021-02-18T09:17:18.310 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-02-18T09:31:26 Research Use Only Page 28 of 28