Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210223-1206-10184 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-02-23T12:14:07.172 Number of Tests: 7 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value C6 (+++) (A) * A 3545 (Standard) Klebsiella pneumoniae 2.44 Klebsiella pneumoniae 2.41 C7 (+++) (A) * A 3546 (Standard) Klebsiella pneumoniae 2.33 Klebsiella pneumoniae 2.32 C8 (+++) (A) * A 3547 (Standard) Klebsiella pneumoniae 2.17 Klebsiella pneumoniae 2.17 C9 (+++) (A) * A 3556 (Standard) Klebsiella pneumoniae 2.21 Klebsiella pneumoniae 2.19 C10 (+++) (A) * A 3585 (Standard) Klebsiella pneumoniae 2.31 Klebsiella pneumoniae 2.30 C11 (+) (B) * B 326 (Standard) Acinetobacter baumannii 1.76 Acinetobacter baumannii 1.71 C12 (-) (C) XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-02-23T12:16:08 Research Use Only Page 1 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Matching Hints Matched Pattern Comment Acinetobacter baumannii 13101_1 CHB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii B389 UFL Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30007T HAM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30008 DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30011 DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii ECI RUH_875 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii ECII RUH_134 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii GEIH_2000 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii LMG 994 HAM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Matching Hints table--continued on next page Report created at 2021-02-23T12:16:08 Research Use Only Page 2 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Report created at 2021-02-23T12:16:08 Research Use Only Page 3 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-02-23T12:16:08 Research Use Only Page 4 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Sample 1 Sample Name: C6 Sample Description: Sample ID: * A 3545 Sample Creation Date/Time: 2021-02-23T12:08:04.785 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.44 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.41 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.35 72407 4 (+++) Klebsiella pneumoniae 37585 PFM 2.33 573 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.32 574 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.31 574 7 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.24 72407 8 (+++) Klebsiella variicola 150519_03 SAH 2.14 244366 9 (+++) Klebsiella pneumoniae 37595 PFM 2.14 573 10 (+++) Klebsiella variicola 150416_01 BRUS 2.08 244366 Report created at 2021-02-23T12:16:08 Research Use Only Page 5 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Sample 2 Sample Name: C7 Sample Description: Sample ID: * A 3546 Sample Creation Date/Time: 2021-02-23T12:08:04.786 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.33 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.32 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.32 573 4 (+++) Klebsiella pneumoniae 37585 PFM 2.24 573 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.24 574 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.20 574 7 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.19 72407 8 (+++) Klebsiella variicola 150416_01 BRUS 2.10 244366 9 (+++) Klebsiella pneumoniae 37595 PFM 2.05 573 10 (+++) Klebsiella variicola 150519_03 SAH 2.00 244366 Report created at 2021-02-23T12:16:08 Research Use Only Page 6 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Sample 3 Sample Name: C8 Sample Description: Sample ID: * A 3547 Sample Creation Date/Time: 2021-02-23T12:08:04.786 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.17 72407 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.17 573 3 (+++) Klebsiella pneumoniae 37585 PFM 2.13 573 4 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.13 72407 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.10 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.08 72407 7 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.01 574 8 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.01 39831 9 (+++) Klebsiella variicola DSM 15968T HAM 2.00 244366 10 (+) Klebsiella pneumoniae 37595 PFM 1.93 573 Report created at 2021-02-23T12:16:08 Research Use Only Page 7 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Sample 4 Sample Name: C9 Sample Description: Sample ID: * A 3556 Sample Creation Date/Time: 2021-02-23T12:08:04.787 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.21 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.19 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.19 573 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.14 574 5 (+++) Klebsiella pneumoniae 37585 PFM 2.13 573 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.09 72407 7 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.05 39831 8 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.03 574 9 (+) Klebsiella variicola TC_4131 MCW 1.97 244366 10 (+) Klebsiella variicola 150519_03 SAH 1.96 244366 Report created at 2021-02-23T12:16:08 Research Use Only Page 8 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Sample 5 Sample Name: C10 Sample Description: Sample ID: * A 3585 Sample Creation Date/Time: 2021-02-23T12:08:04.787 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.31 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.30 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.23 72407 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.15 574 5 (+++) Klebsiella pneumoniae 37585 PFM 2.13 573 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.12 574 7 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.10 72407 8 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.99 39831 9 (+) Klebsiella variicola DSM 15968T DSM 1.99 244366 10 (+) Klebsiella variicola DSM 15968T HAM 1.98 244366 Report created at 2021-02-23T12:16:08 Research Use Only Page 9 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Sample 6 Sample Name: C11 Sample Description: Sample ID: * B 326 Sample Creation Date/Time: 2021-02-23T12:08:04.788 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Acinetobacter baumannii DSM 30007T HAM 1.76 470 2 (+) Acinetobacter baumannii LMG 994 HAM 1.71 470 3 (+) Acinetobacter baumannii 13101_1 CHB 1.71 470 4 (-) Acinetobacter baumannii B389 UFL 1.56 470 5 (-) Acinetobacter baumannii DSM 30011 DSM 1.55 470 6 (-) Acinetobacter baumannii GEIH_2000 HCB 1.50 470 7 (-) Acinetobacter baumannii DSM 30008 DSM 1.42 470 8 (-) Acinetobacter baumannii ECII RUH_134 HCB 1.39 470 9 (-) Acinetobacter baumannii ECI RUH_875 HCB 1.36 470 10 (-) Aspergillus niger 1069 PFM 1.33 5061 Report created at 2021-02-23T12:16:08 Research Use Only Page 10 of 11 Run Identifier: 210223-1206-10184 Run Creation Date/Time: 2021-02-23T12:14:07.172 Sample 7 Sample Name: C12 Sample Description: Sample ID: XXX Sample Creation Date/Time: 2021-02-23T12:08:04.788 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-02-23T12:16:08 Research Use Only Page 11 of 11