Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210312-1226-10199 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-03-12T12:33:23.367 Number of Tests: 10 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value E5 (+++) (A) * B 760 (Standard) Klebsiella pneumoniae 2.18 Klebsiella pneumoniae 2.12 E6 (+++) (A) * B 815 (Standard) Klebsiella pneumoniae 2.03 Klebsiella pneumoniae 2.03 E7 (+++) (A) * B 812 (Standard) Klebsiella pneumoniae 2.27 Klebsiella pneumoniae 2.18 E8 (+++) (A) * B 840 (Standard) Klebsiella pneumoniae 2.21 Klebsiella pneumoniae 2.07 E9 (+) (C) * B 770 (Standard) Pseudomonas aeruginosa 1.99 Klebsiella variicola 1.81 E10 (-) (C) * C XXX (Standard) no peaks found 0.00 no peaks found 0.00 E11 (+++) (A) * C 5446 (Standard) Klebsiella pneumoniae 2.30 Klebsiella pneumoniae 2.26 Result overview table--continued on next page Report created at 2021-03-12T12:36:49 Research Use Only Page 1 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value E12 (+++) (A) * C 5661 (Standard) Klebsiella pneumoniae 2.27 Klebsiella pneumoniae 2.19 F1 (+++) (A) * C 5613 (Standard) Klebsiella pneumoniae 2.34 Klebsiella pneumoniae 2.32 F2 (-) (C) * C XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-03-12T12:36:49 Research Use Only Page 2 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Matching Hints Matched Pattern Comment Klebsiella aerogenes 15282_1 CHB synonym of Enterobacter aerogenes Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Matching Hints table--continued on next page Report created at 2021-03-12T12:36:49 Research Use Only Page 3 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Report created at 2021-03-12T12:36:49 Research Use Only Page 4 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-03-12T12:36:49 Research Use Only Page 5 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 1 Sample Name: E5 Sample Description: Sample ID: * B 760 Sample Creation Date/Time: 2021-03-12T12:29:16.479 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.18 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.12 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.11 574 4 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.04 72407 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.02 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.01 72407 7 (+) Klebsiella variicola 150519_03 SAH 1.98 244366 8 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.97 39831 9 (+) Klebsiella pneumoniae 37585 PFM 1.95 573 10 (+) Klebsiella variicola 37924 PFM 1.91 244366 Report created at 2021-03-12T12:36:49 Research Use Only Page 6 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 2 Sample Name: E6 Sample Description: Sample ID: * B 815 Sample Creation Date/Time: 2021-03-12T12:29:16.480 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.03 72407 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.03 573 3 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.94 574 4 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.91 72407 5 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.88 574 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.86 72407 7 (+) Klebsiella variicola DSM 15968T HAM 1.83 244366 8 (+) Klebsiella pneumoniae 37585 PFM 1.81 573 9 (+) Klebsiella aerogenes 15282_1 CHB 1.80 28451 10 (+) Klebsiella pneumoniae 37595 PFM 1.74 573 Report created at 2021-03-12T12:36:49 Research Use Only Page 7 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 3 Sample Name: E7 Sample Description: Sample ID: * B 812 Sample Creation Date/Time: 2021-03-12T12:29:16.481 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.27 573 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.18 574 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.13 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.11 72407 5 (+++) Klebsiella variicola DSM 15968T HAM 2.09 244366 6 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.08 72407 7 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.02 574 8 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.01 39831 9 (+) Klebsiella pneumoniae 37585 PFM 1.96 573 10 (+) Klebsiella variicola DSM 15968T DSM 1.94 244366 Report created at 2021-03-12T12:36:49 Research Use Only Page 8 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 4 Sample Name: E8 Sample Description: Sample ID: * B 840 Sample Creation Date/Time: 2021-03-12T12:29:16.481 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.21 573 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.07 574 3 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.03 72407 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.02 574 5 (+++) Klebsiella variicola DSM 15968T HAM 2.00 244366 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.99 72407 7 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.96 39831 8 (+) Klebsiella variicola 150519_03 SAH 1.93 244366 9 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.84 72407 10 (+) Klebsiella pneumoniae 37585 PFM 1.82 573 Report created at 2021-03-12T12:36:49 Research Use Only Page 9 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 5 Sample Name: E9 Sample Description: Sample ID: * B 770 Sample Creation Date/Time: 2021-03-12T12:29:16.482 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Pseudomonas aeruginosa ATCC 27853 THL 1.99 287 2 (+) Klebsiella variicola CC_2053 MCW 1.81 244366 3 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.80 72407 4 (+) Pseudomonas aeruginosa 8147_2 CHB 1.78 287 5 (+) Klebsiella variicola NY_1125 MCW 1.78 244366 6 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.76 287 7 (+) Klebsiella variicola DSM 15968T DSM 1.74 244366 8 (+) Klebsiella variicola TC_4131 MCW 1.71 244366 9 (-) Klebsiella pneumoniae RV_BA_03_B LBK 1.69 573 10 (-) Pseudomonas aeruginosa 19955_1 CHB 1.68 287 Report created at 2021-03-12T12:36:49 Research Use Only Page 10 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 6 Sample Name: E10 Sample Description: Sample ID: * C XXX Sample Creation Date/Time: 2021-03-12T12:29:16.482 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-03-12T12:36:49 Research Use Only Page 11 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 7 Sample Name: E11 Sample Description: Sample ID: * C 5446 Sample Creation Date/Time: 2021-03-12T12:29:16.483 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.30 574 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.26 573 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.17 574 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.16 72407 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.15 72407 6 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.14 72407 7 (+++) Klebsiella pneumoniae 37585 PFM 2.04 573 8 (+++) Klebsiella variicola DSM 15968T HAM 2.03 244366 9 (+) Klebsiella variicola 150519_03 SAH 1.97 244366 10 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.96 39831 Report created at 2021-03-12T12:36:49 Research Use Only Page 12 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 8 Sample Name: E12 Sample Description: Sample ID: * C 5661 Sample Creation Date/Time: 2021-03-12T12:29:16.483 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.27 72407 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.19 573 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.19 574 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.19 574 5 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.15 72407 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.09 72407 7 (+++) Klebsiella pneumoniae 37585 PFM 2.08 573 8 (+++) Klebsiella pneumoniae 37595 PFM 2.01 573 9 (+) Klebsiella variicola TC_4131 MCW 1.92 244366 10 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.91 39831 Report created at 2021-03-12T12:36:49 Research Use Only Page 13 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 9 Sample Name: F1 Sample Description: Sample ID: * C 5613 Sample Creation Date/Time: 2021-03-12T12:29:16.484 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.34 574 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.32 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.31 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.31 72407 5 (+++) Klebsiella pneumoniae 37585 PFM 2.30 573 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.29 574 7 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.25 573 8 (+++) Klebsiella pneumoniae 37595 PFM 2.14 573 9 (+++) Klebsiella variicola 150416_01 BRUS 2.11 244366 10 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.09 39831 Report created at 2021-03-12T12:36:49 Research Use Only Page 14 of 15 Run Identifier: 210312-1226-10199 Run Creation Date/Time: 2021-03-12T12:33:23.367 Sample 10 Sample Name: F2 Sample Description: Sample ID: * C XXX Sample Creation Date/Time: 2021-03-12T12:29:16.484 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-03-12T12:36:49 Research Use Only Page 15 of 15