Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210318-1306-10184 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-03-18T13:15:21.595 Number of Tests: 15 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value A1 (+++) (A) * 21 CRC R F 88BILA (Standard) Escherichia coli 2.23 Escherichia coli 2.14 A2 (+) (B) * 21 CRC R F 88SEDA (Standard) Fusobacterium nucleatum 1.84 No Organism Identification Possible 1.63 A3 (+++) (A) * A 5598 (Standard) Klebsiella pneumoniae 2.14 Klebsiella pneumoniae 2.11 A4 (+++) (A) * A 5669 (Standard) Klebsiella pneumoniae 2.28 Klebsiella pneumoniae 2.25 A5 (+++) (A) * A 5684 (Standard) Klebsiella pneumoniae 2.14 Klebsiella pneumoniae 2.13 A6 (+++) (A) * B 234 (Standard) Acinetobacter baumannii 2.33 Acinetobacter baumannii 2.19 A7 (+++) (A) * B 320 (Standard) Klebsiella pneumoniae 2.03 Klebsiella pneumoniae 1.99 Result overview table--continued on next page Report created at 2021-03-18T13:18:29 Research Use Only Page 1 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value A8 (+++) (A) * B 275 (Standard) Klebsiella pneumoniae 2.10 Klebsiella pneumoniae 2.02 A9 (+++) (A) * B 301 (Standard) Klebsiella pneumoniae 2.25 Klebsiella pneumoniae 2.24 A10 (+++) (A) * C 5920 (Standard) Klebsiella pneumoniae 2.32 Klebsiella pneumoniae 2.20 A11 (+++) (A) * C 5980 (Standard) Klebsiella pneumoniae 2.21 Klebsiella pneumoniae 2.13 A12 (+) (B) * C 6094 (Standard) Klebsiella variicola 1.79 Klebsiella pneumoniae 1.74 B1 (+++) (A) * C 6103 (Standard) Klebsiella variicola 2.30 Klebsiella variicola 2.14 B2 (+++) (A) * C 6170 (Standard) Klebsiella pneumoniae 2.52 Klebsiella pneumoniae 2.38 B3 (-) (C) XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-03-18T13:18:29 Research Use Only Page 2 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Matching Hints Matched Pattern Comment Acinetobacter baumannii 13101_1 CHB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii B389 UFL Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30007T HAM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30007T_QC DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30008 DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii DSM 30011 DSM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii GEIH_2000 HCB Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter baumannii LMG 994 HAM Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter nosocomialis LMG 10625 LMG Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Acinetobacter nosocomialis LMG 10626 LMG Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Cronobacter sp LMG 2786 LMG Cronobacter can only be identified on genus level. Escherichia coli ATCC 25922 THL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 35218 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1576 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 30083T HAM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Matching Hints table--continued on next page Report created at 2021-03-18T13:18:29 Research Use Only Page 3 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Matching Hints table--continued from previous page Matched Pattern Comment Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ESBL_EA_RSS_1528T CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli MB11464_1 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli RV412_A1_2010_06a LBK closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Fusobacterium nucleatum 100617_02 PNU Species naviforme / nucleatum of the genus Fusobacterium have very similar patterns: Therefore distinguishing their species is difficult. Fusobacterium nucleatum 110706_B7 LUMC Species naviforme / nucleatum of the genus Fusobacterium have very similar patterns: Therefore distinguishing their species is difficult. Fusobacterium nucleatum BK635_11 ERL Species naviforme / nucleatum of the genus Fusobacterium have very similar patterns: Therefore distinguishing their species is difficult. Fusobacterium nucleatum CCUG 45873 CCUG Species naviforme / nucleatum of the genus Fusobacterium have very similar patterns: Therefore distinguishing their species is difficult. Fusobacterium sp[2] 08_001 N5 IBS Species naviforme / nucleatum of the genus Fusobacterium have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Matching Hints table--continued on next page Report created at 2021-03-18T13:18:29 Research Use Only Page 4 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola CC_2123 MCW closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Report created at 2021-03-18T13:18:29 Research Use Only Page 5 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-03-18T13:18:29 Research Use Only Page 6 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 1 Sample Name: A1 Sample Description: Sample ID: * 21 CRC R F 88BILA Sample Creation Date/Time: 2021-03-18T13:09:18.618 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.23 562 2 (+++) Escherichia coli ATCC 25922 THL 2.14 562 3 (+++) Escherichia coli DSM 682 DSM 2.11 562 4 (+++) Escherichia coli MB11464_1 CHB 2.09 562 5 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.09 562 6 (+++) Escherichia coli DSM 1576 DSM 2.03 562 7 (+++) Escherichia coli Nissl VML 2.00 562 8 (+) Escherichia coli ATCC 35218 CHB 1.95 562 9 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.89 562 10 (+) Escherichia coli DSM 30083T HAM 1.88 562 Report created at 2021-03-18T13:18:29 Research Use Only Page 7 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 2 Sample Name: A2 Sample Description: Sample ID: * 21 CRC R F 88SEDA Sample Creation Date/Time: 2021-03-18T13:09:18.618 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Fusobacterium nucleatum 100617_02 PNU 1.84 851 2 (-) Campylobacter ureolyticus 110324_96 PNU 1.63 827 3 (-) Campylobacter ureolyticus HU33416 PNU 1.60 827 4 (-) Campylobacter ureolyticus DSM 20703T DSM 1.60 827 5 (-) Fusobacterium sp[2] 08_001 N5 IBS 1.45 848 6 (-) Clostridium septicum 1026_NCTC 547T BOG 1.35 1504 7 (-) Campylobacter ureolyticus VA20417_11 ERL 1.34 827 8 (-) Fusobacterium nucleatum CCUG 45873 CCUG 1.34 851 9 (-) Fusobacterium nucleatum 110706_B7 LUMC 1.26 851 10 (-) Fusobacterium nucleatum BK635_11 ERL 1.22 851 Report created at 2021-03-18T13:18:29 Research Use Only Page 8 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 3 Sample Name: A3 Sample Description: Sample ID: * A 5598 Sample Creation Date/Time: 2021-03-18T13:09:18.619 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.14 574 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.11 573 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.10 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.09 72407 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.06 72407 6 (+++) Klebsiella pneumoniae 37585 PFM 2.03 573 7 (+++) Klebsiella variicola DSM 15968T HAM 2.02 244366 8 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.00 39831 9 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.93 574 10 (+) Klebsiella variicola DSM 15968T DSM 1.89 244366 Report created at 2021-03-18T13:18:29 Research Use Only Page 9 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 4 Sample Name: A4 Sample Description: Sample ID: * A 5669 Sample Creation Date/Time: 2021-03-18T13:09:18.619 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.28 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.25 72407 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.22 72407 4 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.21 573 5 (+++) Klebsiella pneumoniae 37585 PFM 2.21 573 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.17 574 7 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.15 574 8 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.01 39831 9 (+) Klebsiella pneumoniae 37595 PFM 1.97 573 10 (+) Klebsiella variicola 150416_01 BRUS 1.90 244366 Report created at 2021-03-18T13:18:29 Research Use Only Page 10 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 5 Sample Name: A5 Sample Description: Sample ID: * A 5684 Sample Creation Date/Time: 2021-03-18T13:09:18.620 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.14 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.13 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.12 573 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.06 574 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.01 72407 6 (+++) Klebsiella variicola DSM 15968T HAM 2.00 244366 7 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.99 574 8 (+) Klebsiella pneumoniae 37585 PFM 1.98 573 9 (+) Klebsiella variicola 37924 PFM 1.87 244366 10 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.85 39831 Report created at 2021-03-18T13:18:29 Research Use Only Page 11 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 6 Sample Name: A6 Sample Description: Sample ID: * B 234 Sample Creation Date/Time: 2021-03-18T13:09:18.620 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Acinetobacter baumannii DSM 30007T HAM 2.33 470 2 (+++) Acinetobacter baumannii B389 UFL 2.19 470 3 (+++) Acinetobacter baumannii DSM 30007T_QC DSM 2.15 470 4 (+++) Acinetobacter baumannii DSM 30008 DSM 2.12 470 5 (+++) Acinetobacter baumannii LMG 994 HAM 2.10 470 6 (+++) Acinetobacter baumannii 13101_1 CHB 2.05 470 7 (+++) Acinetobacter baumannii GEIH_2000 HCB 2.03 470 8 (+) Acinetobacter nosocomialis LMG 10625 LMG 1.93 72607 9 (+) Acinetobacter nosocomialis LMG 10626 LMG 1.90 72607 10 (+) Acinetobacter baumannii DSM 30011 DSM 1.90 470 Report created at 2021-03-18T13:18:29 Research Use Only Page 12 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 7 Sample Name: A7 Sample Description: Sample ID: * B 320 Sample Creation Date/Time: 2021-03-18T13:09:18.621 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.03 573 2 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.99 574 3 (+) Klebsiella variicola DSM 15968T HAM 1.84 244366 4 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.82 574 5 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.71 72407 6 (+) Klebsiella variicola 150519_03 SAH 1.70 244366 7 (+) Klebsiella variicola DSM 15968T DSM 1.70 244366 8 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.70 72407 9 (-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.65 574 10 (-) Klebsiella variicola NY_1064 MCW 1.64 244366 Report created at 2021-03-18T13:18:29 Research Use Only Page 13 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 8 Sample Name: A8 Sample Description: Sample ID: * B 275 Sample Creation Date/Time: 2021-03-18T13:09:18.621 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.10 573 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.02 72407 3 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.99 72407 4 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.98 72407 5 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.90 39831 6 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.89 574 7 (+) Klebsiella variicola DSM 15968T HAM 1.88 244366 8 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.84 574 9 (+) Klebsiella variicola DSM 15968T DSM 1.77 244366 10 (+) Klebsiella pneumoniae 37585 PFM 1.76 573 Report created at 2021-03-18T13:18:29 Research Use Only Page 14 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 9 Sample Name: A9 Sample Description: Sample ID: * B 301 Sample Creation Date/Time: 2021-03-18T13:09:18.622 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.25 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.24 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.16 573 4 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.14 39831 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.13 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.10 72407 7 (+++) Klebsiella pneumoniae 37585 PFM 2.05 573 8 (+) Klebsiella variicola DSM 15968T HAM 1.99 244366 9 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.96 574 10 (+) Klebsiella variicola NY_1125 MCW 1.92 244366 Report created at 2021-03-18T13:18:29 Research Use Only Page 15 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 10 Sample Name: A10 Sample Description: Sample ID: * C 5920 Sample Creation Date/Time: 2021-03-18T13:09:18.622 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.32 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.20 72407 3 (+++) Klebsiella pneumoniae 37585 PFM 2.16 573 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.14 574 5 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.09 573 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.03 72407 7 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.01 574 8 (+) Klebsiella pneumoniae 37595 PFM 1.98 573 9 (+) Klebsiella variicola 150416_01 BRUS 1.88 244366 10 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.87 39831 Report created at 2021-03-18T13:18:29 Research Use Only Page 16 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 11 Sample Name: A11 Sample Description: Sample ID: * C 5980 Sample Creation Date/Time: 2021-03-18T13:09:18.623 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.21 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.13 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.11 574 4 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.09 573 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.06 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.02 72407 7 (+) Klebsiella variicola DSM 15968T HAM 1.95 244366 8 (+) Klebsiella pneumoniae 37585 PFM 1.94 573 9 (+) Klebsiella variicola 150519_03 SAH 1.93 244366 10 (+) Klebsiella variicola DSM 15968T DSM 1.88 244366 Report created at 2021-03-18T13:18:29 Research Use Only Page 17 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 12 Sample Name: A12 Sample Description: Sample ID: * C 6094 Sample Creation Date/Time: 2021-03-18T13:09:18.623 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella variicola DSM 15968T HAM 1.79 244366 2 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.74 72407 3 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.71 72407 4 (-) Klebsiella variicola NY_1125 MCW 1.67 244366 5 (-) Klebsiella variicola TC_4131 MCW 1.66 244366 6 (-) Klebsiella pneumoniae RV_BA_03_B LBK 1.66 573 7 (-) Klebsiella variicola NY_1064 MCW 1.62 244366 8 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.59 574 9 (-) Klebsiella variicola DSM 15968T DSM 1.58 244366 10 (-) Cronobacter sp LMG 2786 LMG 1.54 28141 Report created at 2021-03-18T13:18:29 Research Use Only Page 18 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 13 Sample Name: B1 Sample Description: Sample ID: * C 6103 Sample Creation Date/Time: 2021-03-18T13:09:18.624 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola DSM 15968T DSM 2.30 244366 2 (+++) Klebsiella variicola CC_2053 MCW 2.14 244366 3 (+++) Klebsiella variicola DSM 15968T HAM 2.13 244366 4 (+++) Klebsiella variicola 150416_01 BRUS 2.11 244366 5 (+++) Klebsiella variicola 150519_03 SAH 2.10 244366 6 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.07 573 7 (+++) Klebsiella variicola NY_1125 MCW 2.03 244366 8 (+++) Klebsiella variicola CC_2123 MCW 2.02 244366 9 (+++) Klebsiella variicola TC_4131 MCW 2.00 244366 10 (+) Klebsiella variicola NY_1064 MCW 1.98 244366 Report created at 2021-03-18T13:18:29 Research Use Only Page 19 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 14 Sample Name: B2 Sample Description: Sample ID: * C 6170 Sample Creation Date/Time: 2021-03-18T13:09:18.624 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.52 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.38 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.35 573 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.31 574 5 (+++) Klebsiella pneumoniae 37585 PFM 2.29 573 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.24 72407 7 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.21 574 8 (+++) Klebsiella pneumoniae 37595 PFM 2.10 573 9 (+++) Klebsiella variicola 150519_03 SAH 2.06 244366 10 (+++) Klebsiella variicola 150416_01 BRUS 2.00 244366 Report created at 2021-03-18T13:18:29 Research Use Only Page 20 of 21 Run Identifier: 210318-1306-10184 Run Creation Date/Time: 2021-03-18T13:15:21.595 Sample 15 Sample Name: B3 Sample Description: Sample ID: XXX Sample Creation Date/Time: 2021-03-18T13:09:18.625 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-03-18T13:18:29 Research Use Only Page 21 of 21