Bruker MALDI Biotyper 
Identification Results 


Run Info: 
Run Identifier: 210604-1216-10199 
Comment: 
Operator: maldi@FLEX-PC 
Run Creation Date/Time: 2021-06-04T12:17:19.741 
Number of Tests: 4 
Type: Standard 
BTS-QC: not present 
BTS-QC Position: 
Instrument ID: 269944.01064 
Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 

Result Overview 

Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
D9 
(+++) (A) 
* A 11754 
(Standard) Escherichia coli 2.31 Escherichia coli 2.23 
D10 
(+++) (A) 
* C 12527 
(Standard) Klebsiella pneumoniae 2.27 Klebsiella pneumoniae 2.23 
D11 
(+++) (A) 
* A 11649 
(Standard) Klebsiella oxytoca 2.28 Klebsiella oxytoca 2.25 
D12 
(-) (C) 
XXX 
(Standard) no peaks found 0.00 no peaks found 0.00 

Report created at 2021-06-04T12:19:28 Research Use Only Page 1 of 

9

Run Identifier: 210604-1216-10199 Run Creation Date/Time: 2021-06-04T12:17:19.741 

Matching Hints 

Matched Pattern Comment 
Escherichia coli ATCC 25922 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 25922 
THL 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 35218 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1103_QC 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1576 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 30083T 
HAM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli MB11464_1 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
RV412_A1_2010_06a LBK 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Klebsiella oxytoca 35130 PFM 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Klebsiella oxytoca ATCC 
700324 THL 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Klebsiella oxytoca DSM 5175T 
HAM 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Klebsiella oxytoca ESBL 30298 
PFM 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Matching Hints table--continued on next page 

Report created at 2021-06-04T12:19:28 Research Use Only Page 2 of 

9

Run Identifier: 210604-1216-10199 Run Creation Date/Time: 2021-06-04T12:17:19.741 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Klebsiella oxytoca 
VA20876_1_09 ERL 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Klebsiella oxytoca 
VA20876_2_09 ERL 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Klebsiella oxytoca VA20879_09 
ERL 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Klebsiella oxytoca VA31877_09 
ERL 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Klebsiella pneumoniae 37585 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 37595 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 
RV_BA_03_B LBK closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T DSM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T HAM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae 9295_1 CHB closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T 
HAM 
closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T_QC 
DSM 
closely related to Klebsiella variicola 
Klebsiella variicola 150416_01 
BRUS closely related to Klebsiella pneumoniae 
Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae 
Raoultella ornithinolytica 
MB_18887 CHB 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 
Matching Hints table--continued on next page 

Report created at 2021-06-04T12:19:28 Research Use Only Page 3 of 

9

Run Identifier: 210604-1216-10199 Run Creation Date/Time: 2021-06-04T12:17:19.741 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Raoultella ornithinolytica 
MHNC_54_1 ERL 
Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus 
Raoultella have very similar patterns: Therefore distinguishing their species is 
difficult. 

Report created at 2021-06-04T12:19:28 Research Use Only Page 4 of 

9

Run Identifier: 210604-1216-10199 Run Creation Date/Time: 2021-06-04T12:17:19.741 

Meaning of Score Values 

Range Interpretation Symbols Color 
2.00 - 3.00 High-confidence identification (+++) green 
1.70 - 1.99 Low-confidence identification (+) yellow 
0.00 - 1.69 No Organism Identification Possible (-) red 

Meaning of Consistency Categories (A - C) 

Category Interpretation 
(A) 
High consistency: The best match is a high-confidence identification. The second-best match is (1) a 
high-confidence identification in which the species is identical to the best match, (2) a low-confidence 
identification in which the species or genus is identical to the best match, or (3) a non-identification. 
(B) 
Low consistency: The requirements for high consistency are not met. The best match is a high- or low-
confidence identification. The second-best match is (1) a high- or low-confidence identification in 
which the genus is identical to the best match or (2) a non-identification. 
(C) No consistency: The requirements for high or low consistency are not met. 

Report created at 2021-06-04T12:19:28 Research Use Only Page 5 of 

9

Run Identifier: 210604-1216-10199 Run Creation Date/Time: 2021-06-04T12:17:19.741 


Sample 1 

Sample Name: D9 
Sample Description: 
Sample ID: * A 11754 
Sample Creation Date/Time: 2021-06-04T12:17:19.772 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli DSM 682 DSM 2.31 562 
2 
(+++) Escherichia coli ATCC 25922 THL 2.23 562 
3 
(+++) Escherichia coli DSM 1576 DSM 2.19 562 
4 
(+++) Escherichia coli MB11464_1 CHB 2.17 562 
5 
(+++) Escherichia coli RV412_A1_2010_06a LBK 2.12 562 
6 
(+++) Escherichia coli ATCC 25922 CHB 2.08 562 
7 
(+++) Escherichia coli DH5alpha BRL 2.07 562 
8 
(+++) Escherichia coli DSM 30083T HAM 2.07 562 
9 
(+++) Escherichia coli DSM 1103_QC DSM 2.03 562 
10 
(+++) Escherichia coli ATCC 35218 CHB 2.02 562 

Report created at 2021-06-04T12:19:28 Research Use Only Page 6 of 

9

Run Identifier: 210604-1216-10199 Run Creation Date/Time: 2021-06-04T12:17:19.741 


Sample 2 

Sample Name: D10 
Sample Description: 
Sample ID: * C 12527 
Sample Creation Date/Time: 2021-06-04T12:17:19.773 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.27 72407 
2 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.23 72407 
3 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.20 574 
4 
(+++) Klebsiella pneumoniae 37585 PFM 2.17 573 
5 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.12 574 
6 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.10 573 
7 
(+++) Klebsiella pneumoniae 37595 PFM 2.09 573 
8 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.02 72407 
9 
(+) Klebsiella variicola 150416_01 BRUS 1.96 244366 
10 
(+) Klebsiella variicola 37924 PFM 1.88 244366 

Report created at 2021-06-04T12:19:28 Research Use Only Page 7 of 

9

Run Identifier: 210604-1216-10199 Run Creation Date/Time: 2021-06-04T12:17:19.741 


Sample 3 

Sample Name: D11 
Sample Description: 
Sample ID: * A 11649 
Sample Creation Date/Time: 2021-06-04T12:17:19.773 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella oxytoca VA20879_09 ERL 2.28 571 
2 
(+++) Klebsiella oxytoca ATCC 700324 THL 2.25 571 
3 
(+++) Klebsiella oxytoca VA31877_09 ERL 2.20 571 
4 
(+++) Klebsiella oxytoca VA20876_2_09 ERL 2.20 571 
5 
(+++) Klebsiella oxytoca VA20876_1_09 ERL 2.17 571 
6 
(+++) Raoultella ornithinolytica MB_18887 CHB 2.15 54291 
7 
(+++) Klebsiella oxytoca 35130 PFM 2.14 571 
8 
(+++) Klebsiella oxytoca ESBL 30298 PFM 2.05 571 
9 
(+++) Klebsiella oxytoca DSM 5175T HAM 2.03 571 
10 
(+) Raoultella ornithinolytica MHNC_54_1 ERL 1.90 54291 

Report created at 2021-06-04T12:19:28 Research Use Only Page 8 of 

9

Run Identifier: 210604-1216-10199 Run Creation Date/Time: 2021-06-04T12:17:19.741 


Sample 4 

Sample Name: D12 
Sample Description: 
Sample ID: XXX 
Sample Creation Date/Time: 2021-06-04T12:17:19.774 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based C 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(-) no peaks found 0.00 -
2 
(-) no peaks found 0.00 -
3 
(-) no peaks found 0.00 -
4 
(-) no peaks found 0.00 -
5 
(-) no peaks found 0.00 -
6 
(-) no peaks found 0.00 -
7 
(-) no peaks found 0.00 -
8 
(-) no peaks found 0.00 -
9 
(-) no peaks found 0.00 -
10 
(-) no peaks found 0.00 -

Report created at 2021-06-04T12:19:28 Research Use Only Page 9 of 

9