Bruker MALDI Biotyper 
Identification Results 


Run Info: 
Run Identifier: 210617-1007-10113 
Comment: 
Operator: maldi@FLEX-PC 
Run Creation Date/Time: 2021-06-17T10:10:22.748 
Number of Tests: 4 
Type: Standard 
BTS-QC: not present 
BTS-QC Position: 
Instrument ID: 269944.01064 
Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 

Result Overview 

Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
D2 
(+++) (A) 
* A 
12580ESCO 
(Standard) 
Escherichia coli 2.02 Escherichia coli 1.98 
D3 
(+++) (A) 
* C 
13407ESCO 
(Standard) 
Escherichia coli 2.23 Escherichia coli 2.22 
D4 
(+++) (A) 
* A 12610 
(Standard) Proteus mirabilis 2.06 Proteus mirabilis 2.05 
D5 
(-) (C) 
* XXX 
(Standard) no peaks found 0.00 no peaks found 0.00 

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Run Identifier: 210617-1007-10113 Run Creation Date/Time: 2021-06-17T10:10:22.748 

Matching Hints 

Matched Pattern Comment 
Escherichia coli ATCC 25922 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 25922 
THL 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 35218 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli B421 UFL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1103_QC 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1576 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 30083T 
HAM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli MB11464_1 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
RV412_A1_2010_06a LBK 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Proteus hauseri CC_2695 MCW Species hauseri / penneri / vulgaris of the genus Proteus have very similar patterns: 
Therefore distinguishing their species is difficult. 

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Run Identifier: 210617-1007-10113 Run Creation Date/Time: 2021-06-17T10:10:22.748 

Meaning of Score Values 

Range Interpretation Symbols Color 
2.00 - 3.00 High-confidence identification (+++) green 
1.70 - 1.99 Low-confidence identification (+) yellow 
0.00 - 1.69 No Organism Identification Possible (-) red 

Meaning of Consistency Categories (A - C) 

Category Interpretation 
(A) 
High consistency: The best match is a high-confidence identification. The second-best match is (1) a 
high-confidence identification in which the species is identical to the best match, (2) a low-confidence 
identification in which the species or genus is identical to the best match, or (3) a non-identification. 
(B) 
Low consistency: The requirements for high consistency are not met. The best match is a high- or low-
confidence identification. The second-best match is (1) a high- or low-confidence identification in 
which the genus is identical to the best match or (2) a non-identification. 
(C) No consistency: The requirements for high or low consistency are not met. 

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Run Identifier: 210617-1007-10113 Run Creation Date/Time: 2021-06-17T10:10:22.748 


Sample 1 

Sample Name: D2 
Sample Description: 
Sample ID: * A 12580ESCO 
Sample Creation Date/Time: 2021-06-17T10:08:23.580 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli MB11464_1 CHB 2.02 562 
2 
(+) Escherichia coli DSM 1103_QC DSM 1.98 562 
3 
(+) Escherichia coli DSM 682 DSM 1.98 562 
4 
(+) Escherichia coli ATCC 25922 CHB 1.85 562 
5 
(+) Escherichia coli ATCC 25922 THL 1.85 562 
6 
(+) Escherichia coli DSM 30083T HAM 1.84 562 
7 
(+) Escherichia coli DSM 1576 DSM 1.83 562 
8 
(+) Escherichia coli Nissl VML 1.83 562 
9 
(+) Escherichia coli B421 UFL 1.79 562 
10 
(+) Escherichia coli ATCC 35218 CHB 1.79 562 

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Run Identifier: 210617-1007-10113 Run Creation Date/Time: 2021-06-17T10:10:22.748 


Sample 2 

Sample Name: D3 
Sample Description: 
Sample ID: * C 13407ESCO 
Sample Creation Date/Time: 2021-06-17T10:08:23.581 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli MB11464_1 CHB 2.23 562 
2 
(+++) Escherichia coli ATCC 25922 THL 2.22 562 
3 
(+++) Escherichia coli DH5alpha BRL 2.19 562 
4 
(+++) Escherichia coli RV412_A1_2010_06a LBK 2.17 562 
5 
(+++) Escherichia coli DSM 30083T HAM 2.14 562 
6 
(+++) Escherichia coli ATCC 35218 CHB 2.13 562 
7 
(+++) Escherichia coli Nissl VML 2.07 562 
8 
(+++) Escherichia coli DSM 682 DSM 2.04 562 
9 
(+) Escherichia coli DSM 1103_QC DSM 1.99 562 
10 
(+) Escherichia coli ATCC 25922 CHB 1.91 562 

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Run Identifier: 210617-1007-10113 Run Creation Date/Time: 2021-06-17T10:10:22.748 


Sample 3 

Sample Name: D4 
Sample Description: 
Sample ID: * A 12610 
Sample Creation Date/Time: 2021-06-17T10:08:23.581 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Proteus mirabilis DSM 18254 DSM 2.06 584 
2 
(+++) Proteus mirabilis DSM 50903 DSM 2.05 584 
3 
(+) Proteus mirabilis 9482_2 CHB 1.99 584 
4 
(+) Proteus mirabilis DSM 46227 DSM 1.96 584 
5 
(+) Proteus mirabilis 13210_1 CHB 1.94 584 
6 
(+) Proteus mirabilis DSM 788 DSM 1.92 584 
7 
(+) Proteus mirabilis (PX) 22086112 MLD 1.90 584 
8 
(+) Proteus mirabilis DSM 30115 DSM 1.83 584 
9 
(+) Proteus mirabilis RV412_A1_2010_06b LBK 1.82 584 
10 
(-) Proteus hauseri CC_2695 MCW 1.47 183417 

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Run Identifier: 210617-1007-10113 Run Creation Date/Time: 2021-06-17T10:10:22.748 


Sample 4 

Sample Name: D5 
Sample Description: 
Sample ID: * XXX 
Sample Creation Date/Time: 2021-06-17T10:08:23.582 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based C 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(-) no peaks found 0.00 -
2 
(-) no peaks found 0.00 -
3 
(-) no peaks found 0.00 -
4 
(-) no peaks found 0.00 -
5 
(-) no peaks found 0.00 -
6 
(-) no peaks found 0.00 -
7 
(-) no peaks found 0.00 -
8 
(-) no peaks found 0.00 -
9 
(-) no peaks found 0.00 -
10 
(-) no peaks found 0.00 -

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