Bruker MALDI Biotyper 
Identification Results 


Run Info: 
Run Identifier: 210629-0951-10199 
Comment: 
Operator: maldi@FLEX-PC 
Run Creation Date/Time: 2021-06-29T10:03:07.732 
Number of Tests: 8 
Type: Standard 
BTS-QC: not present 
BTS-QC Position: 
Instrument ID: 269944.01064 
Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 
Result Overview 

Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
C3 
(+++) (A) 
* C 14123 
(Standard) Escherichia coli 2.47 Escherichia coli 2.35 
C4 
(+++) (A) 
* C 14129ACIN 
(Standard) 
Acinetobacter baumannii 2.38 Acinetobacter baumannii 2.32 
C5 
(+++) (A) 
* C 14153 
(Standard) Escherichia coli 2.30 Escherichia coli 2.20 
C6 
(+++) (A) 
* C 14129-KL 
(Standard) Klebsiella pneumoniae 2.44 Klebsiella pneumoniae 2.37 
C7 
(+++) (A) 
* C 14046 
(Standard) Klebsiella pneumoniae 2.49 Klebsiella pneumoniae 2.44 
C8 
(+++) (A) 
* C 14047 
(Standard) Escherichia coli 2.38 Escherichia coli 2.26 
C9 
(+++) (A) 
* C 14113 
(Standard) Acinetobacter baumannii 2.43 Acinetobacter baumannii 2.38 
Result overview table--continued on next page 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 

Result overview table--continued from previous page 
Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
C10 
(-) (C) 
* XXX 
(Standard) no peaks found 0.00 no peaks found 0.00 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 

Matching Hints 

Matched Pattern Comment 
Acinetobacter baumannii 
13101_1 CHB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii B389 
UFL 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii DSM 
30007T HAM 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii DSM 
30008 DSM 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii DSM 
30011 DSM 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii ECII 
RUH_134 HCB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii ECIII 
RUH_5875 HCB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii 
GEIH_2000 HCB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii LMG 
994 HAM 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter nosocomialis 
LMG 10626 LMG 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Escherichia coli ATCC 25922 
THL 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 35218 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1103_QC 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1576 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 30083T 
HAM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Matching Hints table--continued on next page 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
ESBL_EA_RSS_1528T CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli MB11464_1 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
RV412_A1_2010_06a LBK 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Klebsiella pneumoniae 37585 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 37595 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 
RV_BA_03_B LBK closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T DSM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T HAM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae 9295_1 CHB closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T 
HAM 
closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T_QC 
DSM 
closely related to Klebsiella variicola 
Klebsiella variicola 150416_01 
BRUS closely related to Klebsiella pneumoniae 
Klebsiella variicola 150519_03 
SAH closely related to Klebsiella pneumoniae 
Klebsiella variicola NY_1064 
MCW closely related to Klebsiella pneumoniae 
Matching Hints table--continued on next page 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Klebsiella variicola TC_4131 
MCW closely related to Klebsiella pneumoniae 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 

Meaning of Score Values 

Range Interpretation Symbols Color 
2.00 - 3.00 High-confidence identification (+++) green 
1.70 - 1.99 Low-confidence identification (+) yellow 
0.00 - 1.69 No Organism Identification Possible (-) red 

Meaning of Consistency Categories (A - C) 

Category Interpretation 
(A) 
High consistency: The best match is a high-confidence identification. The second-best match is (1) a 
high-confidence identification in which the species is identical to the best match, (2) a low-confidence 
identification in which the species or genus is identical to the best match, or (3) a non-identification. 
(B) 
Low consistency: The requirements for high consistency are not met. The best match is a high- or low-
confidence identification. The second-best match is (1) a high- or low-confidence identification in 
which the genus is identical to the best match or (2) a non-identification. 
(C) No consistency: The requirements for high or low consistency are not met. 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 


Sample 1 

Sample Name: C3 
Sample Description: 
Sample ID: * C 14123 
Sample Creation Date/Time: 2021-06-29T09:54:43.755 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli ATCC 35218 CHB 2.47 562 
2 
(+++) Escherichia coli MB11464_1 CHB 2.35 562 
3 
(+++) Escherichia coli DH5alpha BRL 2.34 562 
4 
(+++) Escherichia coli DSM 30083T HAM 2.30 562 
5 
(+++) Escherichia coli Nissl VML 2.30 562 
6 
(+++) Escherichia coli ATCC 25922 THL 2.28 562 
7 
(+++) Escherichia coli RV412_A1_2010_06a LBK 2.27 562 
8 
(+++) Escherichia coli DSM 682 DSM 2.12 562 
9 
(+++) Escherichia coli DSM 1576 DSM 2.10 562 
10 
(+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.09 562 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 


Sample 2 

Sample Name: C4 
Sample Description: 
Sample ID: * C 14129-ACIN 
Sample Creation Date/Time: 2021-06-29T09:54:43.755 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Acinetobacter baumannii LMG 994 HAM 2.38 470 
2 
(+++) Acinetobacter baumannii DSM 30007T HAM 2.32 470 
3 
(+++) Acinetobacter baumannii DSM 30008 DSM 2.30 470 
4 
(+++) Acinetobacter baumannii DSM 30011 DSM 2.30 470 
5 
(+++) Acinetobacter baumannii GEIH_2000 HCB 2.29 470 
6 
(+++) Acinetobacter baumannii 13101_1 CHB 2.20 470 
7 
(+++) Acinetobacter baumannii ECII RUH_134 HCB 2.19 470 
8 
(+++) Acinetobacter baumannii B389 UFL 2.14 470 
9 
(+++) Acinetobacter baumannii ECIII RUH_5875 HCB 2.04 470 
10 
(+++) Acinetobacter nosocomialis LMG 10626 LMG 2.02 72607 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 


Sample 3 

Sample Name: C5 
Sample Description: 
Sample ID: * C 14153 
Sample Creation Date/Time: 2021-06-29T09:54:43.756 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli MB11464_1 CHB 2.30 562 
2 
(+++) Escherichia coli ATCC 25922 THL 2.20 562 
3 
(+++) Escherichia coli DH5alpha BRL 2.19 562 
4 
(+++) Escherichia coli DSM 1576 DSM 2.16 562 
5 
(+++) Escherichia coli DSM 30083T HAM 2.16 562 
6 
(+++) Escherichia coli DSM 1103_QC DSM 2.15 562 
7 
(+++) Escherichia coli DSM 682 DSM 2.14 562 
8 
(+++) Escherichia coli Nissl VML 2.10 562 
9 
(+++) Escherichia coli RV412_A1_2010_06a LBK 2.09 562 
10 
(+++) Escherichia coli ATCC 35218 CHB 2.06 562 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 


Sample 4 

Sample Name: C6 
Sample Description: 
Sample ID: * C 14129-KL 
Sample Creation Date/Time: 2021-06-29T09:54:43.756 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.44 72407 
2 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.37 72407 
3 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.37 574 
4 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.18 574 
5 
(+++) Klebsiella pneumoniae 37585 PFM 2.17 573 
6 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.16 573 
7 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.14 72407 
8 
(+++) Klebsiella pneumoniae 37595 PFM 2.11 573 
9 
(+) Klebsiella variicola TC_4131 MCW 1.98 244366 
10 
(+) Klebsiella variicola NY_1064 MCW 1.95 244366 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 


Sample 5 

Sample Name: C7 
Sample Description: 
Sample ID: * C 14046 
Sample Creation Date/Time: 2021-06-29T09:54:43.757 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.49 72407 
2 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.44 574 
3 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.40 72407 
4 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.37 573 
5 
(+++) Klebsiella pneumoniae 37585 PFM 2.34 573 
6 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.27 574 
7 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.26 72407 
8 
(+++) Klebsiella pneumoniae 37595 PFM 2.22 573 
9 
(+++) Klebsiella variicola 150416_01 BRUS 2.19 244366 
10 
(+++) Klebsiella variicola 150519_03 SAH 2.14 244366 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 


Sample 6 

Sample Name: C8 
Sample Description: 
Sample ID: * C 14047 
Sample Creation Date/Time: 2021-06-29T09:54:43.757 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli MB11464_1 CHB 2.38 562 
2 
(+++) Escherichia coli ATCC 25922 THL 2.26 562 
3 
(+++) Escherichia coli DH5alpha BRL 2.25 562 
4 
(+++) Escherichia coli RV412_A1_2010_06a LBK 2.24 562 
5 
(+++) Escherichia coli DSM 30083T HAM 2.19 562 
6 
(+++) Escherichia coli Nissl VML 2.13 562 
7 
(+++) Escherichia coli DSM 1103_QC DSM 2.12 562 
8 
(+++) Escherichia coli DSM 682 DSM 2.10 562 
9 
(+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.08 562 
10 
(+++) Escherichia coli ATCC 35218 CHB 2.08 562 

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Run Identifier: 210629-0951-10199 Run Creation Date/Time: 2021-06-29T10:03:07.732 


Sample 7 

Sample Name: C9 
Sample Description: 
Sample ID: * C 14113 
Sample Creation Date/Time: 2021-06-29T09:54:43.758 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Acinetobacter baumannii LMG 994 HAM 2.43 470 
2 
(+++) Acinetobacter baumannii DSM 30007T HAM 2.38 470 
3 
(+++) Acinetobacter baumannii DSM 30011 DSM 2.34 470 
4 
(+++) Acinetobacter baumannii GEIH_2000 HCB 2.27 470 
5 
(+++) Acinetobacter baumannii DSM 30008 DSM 2.25 470 
6 
(+++) Acinetobacter baumannii 13101_1 CHB 2.25 470 
7 
(+++) Acinetobacter baumannii ECII RUH_134 HCB 2.23 470 
8 
(+++) Acinetobacter baumannii B389 UFL 2.16 470 
9 
(+++) Acinetobacter baumannii ECIII RUH_5875 HCB 2.05 470 
10 
(+++) Acinetobacter nosocomialis LMG 10626 LMG 2.02 72607 

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Sample 8 

Sample Name: C10 
Sample Description: 
Sample ID: * XXX 
Sample Creation Date/Time: 2021-06-29T09:54:43.759 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based C 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(-) no peaks found 0.00 -
2 
(-) no peaks found 0.00 -
3 
(-) no peaks found 0.00 -
4 
(-) no peaks found 0.00 -
5 
(-) no peaks found 0.00 -
6 
(-) no peaks found 0.00 -
7 
(-) no peaks found 0.00 -
8 
(-) no peaks found 0.00 -
9 
(-) no peaks found 0.00 -
10 
(-) no peaks found 0.00 -

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