Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210702-0931-10199 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-07-02T09:37:17.860 Number of Tests: 3 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value F4 (+++) (A) * C 14427 PSAE (Standard) Pseudomonas aeruginosa 2.28 Pseudomonas aeruginosa 2.13 F5 (+++) (A) * A 12370 KLPN (Standard) Klebsiella pneumoniae 2.31 Klebsiella pneumoniae 2.15 F6 (-) (C) * XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-07-02T09:38:48 Research Use Only Page 1 of 6 Run Identifier: 210702-0931-10199 Run Creation Date/Time: 2021-07-02T09:37:17.860 Matching Hints Matched Pattern Comment Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Report created at 2021-07-02T09:38:48 Research Use Only Page 2 of 6 Run Identifier: 210702-0931-10199 Run Creation Date/Time: 2021-07-02T09:37:17.860 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-07-02T09:38:48 Research Use Only Page 3 of 6 Run Identifier: 210702-0931-10199 Run Creation Date/Time: 2021-07-02T09:37:17.860 Sample 1 Sample Name: F4 Sample Description: Sample ID: * C 14427 PSAE Sample Creation Date/Time: 2021-07-02T09:37:17.917 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.28 287 2 (+++) Pseudomonas aeruginosa DSM 50071T HAM 2.13 287 3 (+++) Pseudomonas aeruginosa DSM 1117 DSM 2.04 287 4 (+++) Pseudomonas aeruginosa 8147_2 CHB 2.03 287 5 (+) Pseudomonas aeruginosa 19955_1 CHB 1.95 287 6 (+) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.91 287 7 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.88 287 8 (+) Pseudomonas aeruginosa LMG 8029 LMG 1.88 287 9 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.67 287 10 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.63 198616 Report created at 2021-07-02T09:38:48 Research Use Only Page 4 of 6 Run Identifier: 210702-0931-10199 Run Creation Date/Time: 2021-07-02T09:37:17.860 Sample 2 Sample Name: F5 Sample Description: Sample ID: * A 12370 KLPN Sample Creation Date/Time: 2021-07-02T09:37:17.917 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.31 574 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.15 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.10 573 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.10 574 5 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.09 72407 6 (+++) Klebsiella pneumoniae 37585 PFM 2.06 573 7 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.02 72407 8 (+) Klebsiella pneumoniae 37595 PFM 1.98 573 9 (+) Klebsiella variicola NY_1064 MCW 1.96 244366 10 (+) Klebsiella variicola CC_2053 MCW 1.95 244366 Report created at 2021-07-02T09:38:48 Research Use Only Page 5 of 6 Run Identifier: 210702-0931-10199 Run Creation Date/Time: 2021-07-02T09:37:17.860 Sample 3 Sample Name: F6 Sample Description: Sample ID: * XXX Sample Creation Date/Time: 2021-07-02T09:37:17.918 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-07-02T09:38:48 Research Use Only Page 6 of 6