Bruker MALDI Biotyper 
Identification Results 


Run Info: 
Run Identifier: 210707-1005-10199 
Comment: 
Operator: maldi@FLEX-PC 
Run Creation Date/Time: 2021-07-07T10:12:11.236 
Number of Tests: 6 
Type: Standard 
BTS-QC: not present 
BTS-QC Position: 
Instrument ID: 269944.01064 
Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 

Result Overview 

Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
F6 
(+++) (A) 
* A 13703 
(Standard) Serratia marcescens 2.24 Serratia marcescens 2.23 
F7 
(+++) (A) 
* C 14567KLPN 
(Standard) 
Klebsiella pneumoniae 2.02 Klebsiella pneumoniae 1.99 
F8 
(+++) (A) 
* C 14567PSAE 
(Standard) 
Pseudomonas aeruginosa 2.27 Pseudomonas aeruginosa 2.21 
F9 
(+++) (A) 
* A 13681 
(Standard) Klebsiella pneumoniae 2.15 Klebsiella pneumoniae 2.14 
F10 
(+++) (A) 
* C 14551 
(Standard) Escherichia coli 2.18 Escherichia coli 2.12 
F11 
(-) (C) 
* XXX 
(Standard) no peaks found 0.00 no peaks found 0.00 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 

Matching Hints 

Matched Pattern Comment 
Escherichia coli ATCC 25922 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 25922 
THL 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 35218 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli B421 UFL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 30083T 
HAM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
ESBL_EA_RSS_1528T CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli MB11464_1 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
RV412_A1_2010_06a LBK 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Klebsiella pneumoniae 37585 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 37595 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 
RV_BA_03_B LBK closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T DSM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T HAM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae 9295_1 CHB closely related to Klebsiella variicola 
Matching Hints table--continued on next page 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T 
HAM 
closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T_QC 
DSM 
closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
rhinoscleromatis DSM 16231T 
HAM 
closely related to Klebsiella variicola 
Klebsiella variicola 150416_01 
BRUS closely related to Klebsiella pneumoniae 
Klebsiella variicola CC_2053 
MCW closely related to Klebsiella pneumoniae 
Klebsiella variicola DSM 
15968T DSM closely related to Klebsiella pneumoniae 
Klebsiella variicola TC_4131 
MCW closely related to Klebsiella pneumoniae 
Serratia marcescens (PX) 
24086109 MLD 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens 13103_1 
CHB 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens DSM 12481 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens DSM 12483 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens DSM 12485 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens DSM 30122 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens ssp 
marcescens DSM 30121T DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens ssp 
sakuensis CIP 107489T HAM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Matching Hints table--continued on next page 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Serratia ureilytica DSM 16952T 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 

Meaning of Score Values 

Range Interpretation Symbols Color 
2.00 - 3.00 High-confidence identification (+++) green 
1.70 - 1.99 Low-confidence identification (+) yellow 
0.00 - 1.69 No Organism Identification Possible (-) red 

Meaning of Consistency Categories (A - C) 

Category Interpretation 
(A) 
High consistency: The best match is a high-confidence identification. The second-best match is (1) a 
high-confidence identification in which the species is identical to the best match, (2) a low-confidence 
identification in which the species or genus is identical to the best match, or (3) a non-identification. 
(B) 
Low consistency: The requirements for high consistency are not met. The best match is a high- or low-
confidence identification. The second-best match is (1) a high- or low-confidence identification in 
which the genus is identical to the best match or (2) a non-identification. 
(C) No consistency: The requirements for high or low consistency are not met. 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 


Sample 1 

Sample Name: F6 
Sample Description: 
Sample ID: * A 13703 
Sample Creation Date/Time: 2021-07-07T10:12:11.309 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Serratia marcescens 13103_1 CHB 2.24 615 
2 
(+++) Serratia marcescens ssp marcescens DSM 30121T DSM 2.23 211759 
3 
(+++) Serratia marcescens DSM 12481 DSM 2.19 615 
4 
(+++) Serratia marcescens DSM 30122 DSM 2.18 615 
5 
(+++) Serratia marcescens (PX) 24086109 MLD 2.14 615 
6 
(+++) Serratia marcescens DSM 12485 DSM 2.12 615 
7 
(+++) Serratia marcescens DSM 12483 DSM 2.07 615 
8 
(+) Serratia marcescens ssp sakuensis CIP 107489T HAM 1.96 211760 
9 
(+) Serratia ureilytica DSM 16952T DSM 1.90 300181 
10 
(+) Serratia entomophila DSM 12358T DSM 1.83 42906 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 


Sample 2 

Sample Name: F7 
Sample Description: 
Sample ID: * C 14567-KLPN 
Sample Creation Date/Time: 2021-07-07T10:12:11.310 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.02 72407 
2 
(+) Klebsiella pneumoniae RV_BA_03_B LBK 1.99 573 
3 
(+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.97 574 
4 
(+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.93 574 
5 
(+) Klebsiella variicola 150416_01 BRUS 1.90 244366 
6 
(+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.86 72407 
7 
(+) Klebsiella pneumoniae 37585 PFM 1.85 573 
8 
(+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.83 72407 
9 
(+) Klebsiella variicola DSM 15968T DSM 1.81 244366 
10 
(+) Klebsiella pneumoniae 37595 PFM 1.81 573 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 


Sample 3 

Sample Name: F8 
Sample Description: 
Sample ID: * C 14567-PSAE 
Sample Creation Date/Time: 2021-07-07T10:12:11.311 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Pseudomonas aeruginosa ATCC 27853 THL 2.27 287 
2 
(+++) Pseudomonas aeruginosa 8147_2 CHB 2.21 287 
3 
(+++) Pseudomonas aeruginosa DSM 50071T HAM 2.17 287 
4 
(+++) Pseudomonas aeruginosa 19955_1 CHB 2.15 287 
5 
(+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.93 287 
6 
(+) Pseudomonas aeruginosa DSM 1128 DSM 1.89 287 
7 
(+) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.86 287 
8 
(+) Pseudomonas aeruginosa DSM 1117 DSM 1.86 287 
9 
(+) Pseudomonas aeruginosa LMG 8029 LMG 1.71 287 
10 
(-) Pseudomonas jinjuensis LMG 21316T HAM 1.34 198616 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 


Sample 4 

Sample Name: F9 
Sample Description: 
Sample ID: * A 13681 
Sample Creation Date/Time: 2021-07-07T10:12:11.311 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.15 72407 
2 
(+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.14 574 
3 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.13 573 
4 
(+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.12 72407 
5 
(+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.04 72407 
6 
(+) Klebsiella variicola CC_2053 MCW 1.96 244366 
7 
(+) Klebsiella pneumoniae 37585 PFM 1.95 573 
8 
(+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.95 574 
9 
(+) Klebsiella variicola TC_4131 MCW 1.93 244366 
10 
(+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.93 39831 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 


Sample 5 

Sample Name: F10 
Sample Description: 
Sample ID: * C 14551 
Sample Creation Date/Time: 2021-07-07T10:12:11.312 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli ATCC 35218 CHB 2.18 562 
2 
(+++) Escherichia coli MB11464_1 CHB 2.12 562 
3 
(+++) Escherichia coli DSM 30083T HAM 2.08 562 
4 
(+++) Escherichia coli ATCC 25922 THL 2.03 562 
5 
(+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.02 562 
6 
(+++) Escherichia coli RV412_A1_2010_06a LBK 2.01 562 
7 
(+) Escherichia coli DH5alpha BRL 1.95 562 
8 
(+) Escherichia coli ATCC 25922 CHB 1.93 562 
9 
(+) Escherichia coli B421 UFL 1.91 562 
10 
(+) Escherichia coli Nissl VML 1.89 562 

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Run Identifier: 210707-1005-10199 Run Creation Date/Time: 2021-07-07T10:12:11.236 


Sample 6 

Sample Name: F11 
Sample Description: 
Sample ID: * XXX 
Sample Creation Date/Time: 2021-07-07T10:12:11.312 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based C 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(-) no peaks found 0.00 -
2 
(-) no peaks found 0.00 -
3 
(-) no peaks found 0.00 -
4 
(-) no peaks found 0.00 -
5 
(-) no peaks found 0.00 -
6 
(-) no peaks found 0.00 -
7 
(-) no peaks found 0.00 -
8 
(-) no peaks found 0.00 -
9 
(-) no peaks found 0.00 -
10 
(-) no peaks found 0.00 -

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