Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210709-0901-10184 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-07-09T09:21:49.920 Number of Tests: 21 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value A1 (+++) (A) * B 13366COLI (Standard) Escherichia coli 2.27 Escherichia coli 2.26 A2 (+++) (A) * B 13366EWLL (Standard) Escherichia coli 2.38 Escherichia coli 2.33 A3 (+++) (A) * B 13367 (Standard) Escherichia coli 2.14 Escherichia coli 2.08 A4 (+++) (A) * B 13372 (Standard) Escherichia coli 2.29 Escherichia coli 2.28 A5 (+++) (A) * B 13302 (Standard) Klebsiella oxytoca 2.30 Klebsiella oxytoca 2.29 A6 (+++) (A) * B 13361 (Standard) Klebsiella oxytoca 2.36 Klebsiella oxytoca 2.34 A7 (+++) (A) * A 13901 (Standard) Pseudomonas aeruginosa 2.35 Pseudomonas aeruginosa 2.08 Result overview table--continued on next page Report created at 2021-07-09T09:27:02 Research Use Only Page 1 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value A8 (+++) (A) * A 13771KLPN (Standard) Klebsiella pneumoniae 2.22 Klebsiella pneumoniae 2.14 A9 (+) (B) * A 13771COLI (Standard) Klebsiella pneumoniae 1.99 Klebsiella pneumoniae 1.92 A10 (+++) (A) * A 13783 (Standard) Klebsiella pneumoniae 2.25 Klebsiella pneumoniae 2.20 A11 (+++) (A) * B 13371 (Standard) Escherichia coli 2.12 Escherichia coli 2.00 A12 (+++) (A) * C 14658 (Standard) Klebsiella oxytoca 2.05 Klebsiella oxytoca 1.92 B1 (+++) (A) * C 14665 (Standard) Pseudomonas aeruginosa 2.31 Pseudomonas aeruginosa 2.01 B2 (+++) (A) * B 13368 (Standard) Escherichia coli 2.24 Escherichia coli 2.22 B3 (+++) (A) * C 14696 (Standard) Klebsiella pneumoniae 2.27 Klebsiella pneumoniae 2.14 B4 (+++) (B) * C 14696SEMA (Standard) Serratia ureilytica 2.11 Serratia marcescens 2.11 B5 (+++) (A) * C 14699 (Standard) Burkholderia multivorans 2.18 Burkholderia multivorans 2.16 B6 (+) (B) * C 14702 (Standard) Klebsiella pneumoniae 1.99 Klebsiella pneumoniae 1.98 B7 (+++) (A) * C 14703 (Standard) Klebsiella pneumoniae 2.11 Klebsiella pneumoniae 1.98 B8 (+++) (A) * C 14750 (Standard) Escherichia coli 2.03 Escherichia coli 1.87 B9 (-) (C) * XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-07-09T09:27:03 Research Use Only Page 2 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Matching Hints Matched Pattern Comment Burkholderia anthina DSM 16086T DSM is a member of Burkholderia cepacia complex Burkholderia anthina LMG 16670 HAM is a member of Burkholderia cepacia complex Burkholderia diffusa DSM 23434T DSM is a member of Burkholderia cepacia complex Burkholderia dolosa DSM 16088T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans DSM 13243T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans LMG 14293 HAM is a member of Burkholderia cepacia complex Burkholderia multivorans VA40328_07 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721a_08 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721b_08 ERL is a member of Burkholderia cepacia complex Burkholderia vietnamiensis LMG 10929T HAM is a member of Burkholderia cepacia complex Escherichia coli ATCC 25922 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 25922 THL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 35218 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1103_QC DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1576 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Matching Hints table--continued on next page Report created at 2021-07-09T09:27:03 Research Use Only Page 3 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Matching Hints table--continued from previous page Matched Pattern Comment Escherichia coli DSM 30083T HAM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ESBL_EA_RSS_1528T CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli MB11464_1 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli RV412_A1_2010_06a LBK closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli W3350 MMG closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Klebsiella aerogenes 15282_1 CHB synonym of Enterobacter aerogenes Klebsiella oxytoca 35130 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ATCC 700324 THL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca DSM 5175T HAM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ESBL 30298 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_1_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_2_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-07-09T09:27:03 Research Use Only Page 4 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella oxytoca VA20879_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA31877_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Matching Hints table--continued on next page Report created at 2021-07-09T09:27:03 Research Use Only Page 5 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Raoultella ornithinolytica DSM 7464T DSM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MB_18887 CHB Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MHNC_19_3 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica VA10895_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Serratia ficaria 17271986_1 MVD For the species ficaria / vespertilionis of the genus Serratia the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Serratia marcescens (PX) 24086109 MLD Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens 13103_1 CHB Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens DSM 12481 DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens DSM 12485 DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens DSM 30122 DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens ssp marcescens DSM 30121T DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens ssp sakuensis CIP 107489T HAM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-07-09T09:27:03 Research Use Only Page 6 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Matching Hints table--continued from previous page Matched Pattern Comment Serratia ureilytica DSM 16952T DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Report created at 2021-07-09T09:27:03 Research Use Only Page 7 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-07-09T09:27:03 Research Use Only Page 8 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 1 Sample Name: A1 Sample Description: Sample ID: * B 13366-COLI Sample Creation Date/Time: 2021-07-09T09:11:57.026 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.27 562 2 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.26 562 3 (+++) Escherichia coli DSM 682 DSM 2.26 562 4 (+++) Escherichia coli DSM 1576 DSM 2.23 562 5 (+++) Escherichia coli MB11464_1 CHB 2.18 562 6 (+++) Escherichia coli ATCC 25922 THL 2.17 562 7 (+++) Escherichia coli DSM 30083T HAM 2.16 562 8 (+++) Escherichia coli DSM 1103_QC DSM 2.13 562 9 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.11 562 10 (+++) Escherichia coli Nissl VML 2.06 562 Report created at 2021-07-09T09:27:03 Research Use Only Page 9 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 2 Sample Name: A2 Sample Description: Sample ID: * B 13366-EWLL Sample Creation Date/Time: 2021-07-09T09:11:57.027 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 682 DSM 2.38 562 2 (+++) Escherichia coli ATCC 25922 THL 2.33 562 3 (+++) Escherichia coli DSM 1576 DSM 2.30 562 4 (+++) Escherichia coli MB11464_1 CHB 2.30 562 5 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.30 562 6 (+++) Escherichia coli DSM 1103_QC DSM 2.29 562 7 (+++) Escherichia coli ATCC 25922 CHB 2.25 562 8 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.23 562 9 (+++) Escherichia coli DSM 30083T HAM 2.15 562 10 (+++) Escherichia coli DH5alpha BRL 2.12 562 Report created at 2021-07-09T09:27:03 Research Use Only Page 10 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 3 Sample Name: A3 Sample Description: Sample ID: * B 13367 Sample Creation Date/Time: 2021-07-09T09:11:57.027 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.14 562 2 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.08 562 3 (+) Escherichia coli ATCC 25922 THL 1.99 562 4 (+) Escherichia coli DSM 30083T HAM 1.92 562 5 (+) Escherichia coli MB11464_1 CHB 1.91 562 6 (+) Escherichia coli DSM 682 DSM 1.84 562 7 (+) Escherichia coli Nissl VML 1.81 562 8 (+) Escherichia coli DSM 1103_QC DSM 1.80 562 9 (+) Escherichia coli W3350 MMG 1.79 562 10 (+) Escherichia coli ATCC 35218 CHB 1.77 562 Report created at 2021-07-09T09:27:03 Research Use Only Page 11 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 4 Sample Name: A4 Sample Description: Sample ID: * B 13372 Sample Creation Date/Time: 2021-07-09T09:11:57.028 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.29 562 2 (+++) Escherichia coli DH5alpha BRL 2.28 562 3 (+++) Escherichia coli DSM 682 DSM 2.19 562 4 (+++) Escherichia coli ATCC 25922 THL 2.19 562 5 (+++) Escherichia coli MB11464_1 CHB 2.18 562 6 (+++) Escherichia coli Nissl VML 2.16 562 7 (+++) Escherichia coli DSM 30083T HAM 2.15 562 8 (+++) Escherichia coli DSM 1103_QC DSM 2.11 562 9 (+++) Escherichia coli DSM 1576 DSM 2.08 562 10 (+++) Escherichia coli ATCC 35218 CHB 2.02 562 Report created at 2021-07-09T09:27:03 Research Use Only Page 12 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 5 Sample Name: A5 Sample Description: Sample ID: * B 13302 Sample Creation Date/Time: 2021-07-09T09:11:57.028 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca ATCC 700324 THL 2.30 571 2 (+++) Klebsiella oxytoca VA20879_09 ERL 2.29 571 3 (+++) Klebsiella oxytoca VA20876_1_09 ERL 2.28 571 4 (+++) Klebsiella oxytoca 35130 PFM 2.09 571 5 (+++) Klebsiella oxytoca VA31877_09 ERL 2.03 571 6 (+++) Klebsiella oxytoca VA20876_2_09 ERL 2.03 571 7 (+++) Raoultella ornithinolytica DSM 7464T DSM 2.02 54291 8 (+) Raoultella ornithinolytica MB_18887 CHB 1.99 54291 9 (+) Raoultella ornithinolytica VA10895_09 ERL 1.89 54291 10 (+) Klebsiella oxytoca ESBL 30298 PFM 1.88 571 Report created at 2021-07-09T09:27:03 Research Use Only Page 13 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 6 Sample Name: A6 Sample Description: Sample ID: * B 13361 Sample Creation Date/Time: 2021-07-09T09:11:57.029 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca VA20879_09 ERL 2.36 571 2 (+++) Klebsiella oxytoca VA20876_1_09 ERL 2.34 571 3 (+++) Klebsiella oxytoca ATCC 700324 THL 2.32 571 4 (+++) Klebsiella oxytoca VA20876_2_09 ERL 2.15 571 5 (+++) Klebsiella oxytoca 35130 PFM 2.12 571 6 (+++) Raoultella ornithinolytica MB_18887 CHB 2.04 54291 7 (+) Klebsiella oxytoca ESBL 30298 PFM 1.99 571 8 (+) Klebsiella oxytoca VA31877_09 ERL 1.97 571 9 (+) Raoultella ornithinolytica DSM 7464T DSM 1.96 54291 10 (+) Raoultella ornithinolytica VA10895_09 ERL 1.93 54291 Report created at 2021-07-09T09:27:03 Research Use Only Page 14 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 7 Sample Name: A7 Sample Description: Sample ID: * A 13901 Sample Creation Date/Time: 2021-07-09T09:11:57.029 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.35 287 2 (+++) Pseudomonas aeruginosa DSM 50071T HAM 2.08 287 3 (+++) Pseudomonas aeruginosa 8147_2 CHB 2.06 287 4 (+++) Pseudomonas aeruginosa DSM 50071T_QC DSM 2.02 287 5 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.99 287 6 (+) Pseudomonas aeruginosa DSM 1117 DSM 1.97 287 7 (+) Pseudomonas aeruginosa DSM 1128 DSM 1.83 287 8 (+) Pseudomonas aeruginosa LMG 8029 LMG 1.82 287 9 (+) Pseudomonas aeruginosa 19955_1 CHB 1.73 287 10 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.56 198616 Report created at 2021-07-09T09:27:03 Research Use Only Page 15 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 8 Sample Name: A8 Sample Description: Sample ID: * A 13771-KLPN Sample Creation Date/Time: 2021-07-09T09:11:57.030 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.22 72407 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.14 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.10 574 4 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.10 574 5 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.08 573 6 (+++) Klebsiella pneumoniae 37585 PFM 2.03 573 7 (+) Klebsiella pneumoniae 37595 PFM 1.98 573 8 (+) Klebsiella variicola 150416_01 BRUS 1.95 244366 9 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.91 72407 10 (+) Klebsiella variicola 150519_03 SAH 1.86 244366 Report created at 2021-07-09T09:27:03 Research Use Only Page 16 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 9 Sample Name: A9 Sample Description: Sample ID: * A 13771-COLI Sample Creation Date/Time: 2021-07-09T09:11:57.030 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.99 574 2 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.92 72407 3 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.91 573 4 (+) Klebsiella variicola NY_1125 MCW 1.87 244366 5 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.87 72407 6 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.82 574 7 (+) Klebsiella variicola NY_1064 MCW 1.79 244366 8 (+) Klebsiella variicola CC_2053 MCW 1.78 244366 9 (+) Klebsiella variicola DSM 15968T DSM 1.77 244366 10 (+) Klebsiella variicola 37924 PFM 1.76 244366 Report created at 2021-07-09T09:27:03 Research Use Only Page 17 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 10 Sample Name: A10 Sample Description: Sample ID: * A 13783 Sample Creation Date/Time: 2021-07-09T09:11:57.031 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.25 72407 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.20 574 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.18 72407 4 (+++) Klebsiella pneumoniae 37585 PFM 2.14 573 5 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.12 573 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.01 72407 7 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.99 574 8 (+) Klebsiella pneumoniae 37595 PFM 1.94 573 9 (+) Klebsiella variicola 150416_01 BRUS 1.82 244366 10 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.71 39831 Report created at 2021-07-09T09:27:03 Research Use Only Page 18 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 11 Sample Name: A11 Sample Description: Sample ID: * B 13371 Sample Creation Date/Time: 2021-07-09T09:11:57.031 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.12 562 2 (+++) Escherichia coli ATCC 25922 THL 2.00 562 3 (+) Escherichia coli RV412_A1_2010_06a LBK 1.98 562 4 (+) Escherichia coli MB11464_1 CHB 1.98 562 5 (+) Escherichia coli DSM 682 DSM 1.97 562 6 (+) Escherichia coli DSM 30083T HAM 1.92 562 7 (+) Escherichia coli ATCC 35218 CHB 1.89 562 8 (+) Escherichia coli DSM 1576 DSM 1.85 562 9 (+) Escherichia coli DSM 1103_QC DSM 1.84 562 10 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.83 562 Report created at 2021-07-09T09:27:03 Research Use Only Page 19 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 12 Sample Name: A12 Sample Description: Sample ID: * C 14658 Sample Creation Date/Time: 2021-07-09T09:11:57.032 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca VA20876_2_09 ERL 2.05 571 2 (+) Klebsiella oxytoca ATCC 700324 THL 1.92 571 3 (+) Klebsiella oxytoca ESBL 30298 PFM 1.80 571 4 (+) Raoultella ornithinolytica MB_18887 CHB 1.75 54291 5 (+) Klebsiella oxytoca VA20876_1_09 ERL 1.73 571 6 (+) Klebsiella oxytoca VA20879_09 ERL 1.73 571 7 (+) Klebsiella oxytoca VA31877_09 ERL 1.73 571 8 (+) Klebsiella oxytoca DSM 5175T HAM 1.72 571 9 (-) Klebsiella oxytoca 35130 PFM 1.62 571 10 (-) Raoultella ornithinolytica MHNC_19_3 ERL 1.56 54291 Report created at 2021-07-09T09:27:03 Research Use Only Page 20 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 13 Sample Name: B1 Sample Description: Sample ID: * C 14665 Sample Creation Date/Time: 2021-07-09T09:11:57.032 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.31 287 2 (+++) Pseudomonas aeruginosa DSM 1117 DSM 2.01 287 3 (+) Pseudomonas aeruginosa 19955_1 CHB 1.99 287 4 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.92 287 5 (+) Pseudomonas aeruginosa 8147_2 CHB 1.90 287 6 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.89 287 7 (+) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.87 287 8 (+) Pseudomonas jinjuensis LMG 21316T HAM 1.84 198616 9 (+) Pseudomonas aeruginosa LMG 8029 LMG 1.71 287 10 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.69 287 Report created at 2021-07-09T09:27:03 Research Use Only Page 21 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 14 Sample Name: B2 Sample Description: Sample ID: * B 13368 Sample Creation Date/Time: 2021-07-09T09:11:57.033 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 30083T HAM 2.24 562 2 (+++) Escherichia coli ATCC 25922 THL 2.22 562 3 (+++) Escherichia coli MB11464_1 CHB 2.22 562 4 (+++) Escherichia coli DH5alpha BRL 2.19 562 5 (+++) Escherichia coli DSM 1103_QC DSM 2.17 562 6 (+++) Escherichia coli DSM 682 DSM 2.16 562 7 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.16 562 8 (+++) Escherichia coli DSM 1576 DSM 2.12 562 9 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.06 562 10 (+++) Escherichia coli ATCC 25922 CHB 2.01 562 Report created at 2021-07-09T09:27:03 Research Use Only Page 22 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 15 Sample Name: B3 Sample Description: Sample ID: * C 14696 Sample Creation Date/Time: 2021-07-09T09:11:57.033 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.27 574 2 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.14 72407 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.08 573 4 (+++) Klebsiella pneumoniae 37585 PFM 2.07 573 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.07 72407 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.03 72407 7 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.98 574 8 (+) Klebsiella pneumoniae 37595 PFM 1.97 573 9 (+) Klebsiella variicola 150416_01 BRUS 1.94 244366 10 (+) Klebsiella variicola 37924 PFM 1.93 244366 Report created at 2021-07-09T09:27:03 Research Use Only Page 23 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 16 Sample Name: B4 Sample Description: Sample ID: * C 14696-SEMA Sample Creation Date/Time: 2021-07-09T09:11:57.034 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Serratia ureilytica DSM 16952T DSM 2.11 300181 2 (+++) Serratia marcescens ssp marcescens DSM 30121T DSM 2.11 211759 3 (+++) Serratia marcescens 13103_1 CHB 2.05 615 4 (+) Serratia marcescens (PX) 24086109 MLD 1.98 615 5 (+) Serratia marcescens DSM 12481 DSM 1.95 615 6 (+) Serratia marcescens ssp sakuensis CIP 107489T HAM 1.89 211760 7 (+) Serratia marcescens DSM 30122 DSM 1.89 615 8 (+) Serratia entomophila DSM 12358T DSM 1.82 42906 9 (+) Serratia ficaria 17271986_1 MVD 1.71 61651 10 (-) Serratia marcescens DSM 12485 DSM 1.61 615 Report created at 2021-07-09T09:27:03 Research Use Only Page 24 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 17 Sample Name: B5 Sample Description: Sample ID: * C 14699 Sample Creation Date/Time: 2021-07-09T09:11:57.034 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Burkholderia multivorans DSM 13243T DSM 2.18 87883 2 (+++) Burkholderia multivorans VA40721a_08 ERL 2.16 87883 3 (+++) Burkholderia multivorans LMG 14293 HAM 2.15 87883 4 (+++) Burkholderia multivorans VA40328_07 ERL 2.10 87883 5 (+++) Burkholderia dolosa DSM 16088T DSM 2.03 152500 6 (+++) Burkholderia multivorans VA40721b_08 ERL 2.00 87883 7 (+) Burkholderia anthina DSM 16086T DSM 1.89 179879 8 (+) Burkholderia vietnamiensis LMG 10929T HAM 1.88 60552 9 (+) Burkholderia anthina LMG 16670 HAM 1.87 179879 10 (+) Burkholderia diffusa DSM 23434T DSM 1.80 87882 Report created at 2021-07-09T09:27:03 Research Use Only Page 25 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 18 Sample Name: B6 Sample Description: Sample ID: * C 14702 Sample Creation Date/Time: 2021-07-09T09:11:57.035 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.99 72407 2 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.98 72407 3 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.96 574 4 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.82 574 5 (+) Klebsiella pneumoniae 37585 PFM 1.81 573 6 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.77 573 7 (+) Klebsiella variicola 150416_01 BRUS 1.74 244366 8 (+) Klebsiella pneumoniae 37595 PFM 1.71 573 9 (+) Klebsiella variicola 150519_03 SAH 1.70 244366 10 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.66 72407 Report created at 2021-07-09T09:27:03 Research Use Only Page 26 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 19 Sample Name: B7 Sample Description: Sample ID: * C 14703 Sample Creation Date/Time: 2021-07-09T09:11:57.035 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.11 574 2 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.98 72407 3 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.93 574 4 (+) Klebsiella pneumoniae 37585 PFM 1.93 573 5 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.89 72407 6 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.78 573 7 (+) Klebsiella pneumoniae 37595 PFM 1.77 573 8 (+) Klebsiella variicola 150416_01 BRUS 1.73 244366 9 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.72 72407 10 (-) Klebsiella aerogenes 15282_1 CHB 1.68 28451 Report created at 2021-07-09T09:27:03 Research Use Only Page 27 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 20 Sample Name: B8 Sample Description: Sample ID: * C 14750 Sample Creation Date/Time: 2021-07-09T09:11:57.036 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli ATCC 25922 THL 2.03 562 2 (+) Escherichia coli DH5alpha BRL 1.87 562 3 (+) Escherichia coli MB11464_1 CHB 1.82 562 4 (+) Escherichia coli ATCC 25922 CHB 1.81 562 5 (+) Escherichia coli RV412_A1_2010_06a LBK 1.78 562 6 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.78 562 7 (+) Escherichia coli Nissl VML 1.75 562 8 (+) Escherichia coli DSM 682 DSM 1.74 562 9 (+) Escherichia coli DSM 30083T HAM 1.72 562 10 (-) Escherichia coli DSM 1103_QC DSM 1.66 562 Report created at 2021-07-09T09:27:03 Research Use Only Page 28 of 29 Run Identifier: 210709-0901-10184 Run Creation Date/Time: 2021-07-09T09:21:49.920 Sample 21 Sample Name: B9 Sample Description: Sample ID: * XXX Sample Creation Date/Time: 2021-07-09T09:11:57.036 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-07-09T09:27:03 Research Use Only Page 29 of 29