Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210714-0935-10184 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-07-14T10:04:54.823 Number of Tests: 30 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value C7 (+++) (A) * c 14848 (Standard) Escherichia coli 2.43 Escherichia coli 2.40 C8 (+++) (A) * c 14903 (Standard) Escherichia coli 2.33 Escherichia coli 2.33 C9 (+++) (A) * c 14943 (Standard) Burkholderia multivorans 2.11 Burkholderia multivorans 2.03 C10 (+) (B) * c 14938 (Standard) Klebsiella pneumoniae 1.83 Klebsiella pneumoniae 1.78 C11 (+++) (A) * c 14939 (Standard) Klebsiella pneumoniae 2.22 Klebsiella pneumoniae 2.16 C12 (+++) (A) * c 14940 (Standard) Pseudomonas aeruginosa 2.17 Pseudomonas aeruginosa 1.87 D1 (+++) (A) * b 13677 (Standard) Escherichia coli 2.20 Escherichia coli 2.11 Result overview table--continued on next page Report created at 2021-07-14T10:15:49 Research Use Only Page 1 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value D2 (+++) (A) * b 13680-esco (Standard) Escherichia coli 2.27 Escherichia coli 2.22 D3 (+++) (A) * b 13680-esha (Standard) Escherichia coli 2.25 Escherichia coli 2.23 D4 (+++) (C) * b 13687-klox (Standard) Klebsiella oxytoca 2.06 Raoultella ornithinolytica 1.93 D5 (+++) (A) * b 13687-aero (Standard) Klebsiella oxytoca 2.01 Klebsiella oxytoca 1.94 D6 (+++) (A) * b 13611 (Standard) Citrobacter freundii 2.16 Citrobacter freundii 2.15 D7 (+++) (A) * b 13615 (Standard) Escherichia coli 2.06 Escherichia coli 2.02 D8 (+++) (A) * b 13693 (Standard) Escherichia coli 2.25 Escherichia coli 2.22 D9 (+++) (A) * b 13691 (Standard) Klebsiella oxytoca 2.02 Klebsiella oxytoca 2.00 D10 (+++) (B) * b 13676 (Standard) Citrobacter freundii 2.12 Citrobacter braakii 2.00 D11 (+++) (A) * a 14170 (Standard) Klebsiella pneumoniae 2.27 Klebsiella pneumoniae 2.25 D12 (+) (B) * a 14157 (Standard) Enterobacter asburiae 1.97 Enterobacter hormaechei 1.91 E1 (+++) (C) * a 14162 (Standard) Klebsiella oxytoca 2.11 Raoultella ornithinolytica 1.97 E2 (+++) (A) * a 14163 (Standard) Escherichia coli 2.20 Escherichia coli 2.07 E3 (+++) (A) * a 14165 (Standard) Klebsiella pneumoniae 2.15 Klebsiella pneumoniae 2.15 E4 (+++) (A) * a 14166 (Standard) Burkholderia multivorans 2.08 Burkholderia multivorans 2.01 E5 (+++) (C) * a 14156 (Standard) Burkholderia multivorans 2.05 Burkholderia multivorans 1.99 Result overview table--continued on next page Report created at 2021-07-14T10:15:49 Research Use Only Page 2 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value E6 (-) (C) * a 14045-klpn (Standard) No Organism Identification Possible 1.56 No Organism Identification Possible 1.56 E7 (+++) (A) * a 14045-encv (Standard) Enterobacter cloacae 2.01 Enterobacter cloacae 2.01 E8 (+++) (A) * a 14046-psae (Standard) Pseudomonas aeruginosa 2.05 Pseudomonas aeruginosa 2.05 E9 (+++) (A) * a 14046-esco (Standard) Escherichia coli 2.26 Escherichia coli 2.19 E10 (+++) (A) * a 14154 (Standard) Klebsiella pneumoniae 2.30 Klebsiella pneumoniae 2.27 E11 (+++) (C) * a 14156 (Standard) Enterobacter xiangfangensis 2.32 Enterobacter cloacae 2.28 E12 (-) (C) * xxx (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-07-14T10:15:49 Research Use Only Page 3 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Matching Hints Matched Pattern Comment Burkholderia ambifaria LMG 11351 HAM is a member of Burkholderia cepacia complex Burkholderia anthina DSM 16086T DSM is a member of Burkholderia cepacia complex Burkholderia anthina LMG 16670 HAM is a member of Burkholderia cepacia complex Burkholderia cepacia_Group 18875_1 CHB is a member of Burkholderia cepacia complex Burkholderia dolosa DSM 16088T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans DSM 13243T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans LMG 14293 HAM is a member of Burkholderia cepacia complex Burkholderia multivorans VA40328_07 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721a_08 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721b_08 ERL is a member of Burkholderia cepacia complex Burkholderia pyrrocinia LMG 14191T HAM is a member of Burkholderia cepacia complex Burkholderia vietnamiensis LMG 10929T HAM is a member of Burkholderia cepacia complex Citrobacter braakii 20663_2 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter braakii 9314_2 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii 13158_2 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii 22054_1 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-07-14T10:15:49 Research Use Only Page 4 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Matching Hints table--continued from previous page Matched Pattern Comment Citrobacter freundii DSM 15979 DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii DSM 30039T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii DSM 30039T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii LMG 21265 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter gillenii CCUG 2749 CCUG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter gillenii DSM 13694T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter youngae DSM 17578T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Enterobacter asburiae DSM 17506T DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101086 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101087 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101088 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101090 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101091 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101092 DSM is a member of Enterobacter cloacae complex Enterobacter cloacae 13159_1 CHB is a member of Enterobacter cloacae complex Enterobacter cloacae 20105_2 CHB is a member of Enterobacter cloacae complex Matching Hints table--continued on next page Report created at 2021-07-14T10:15:49 Research Use Only Page 5 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Matching Hints table--continued from previous page Matched Pattern Comment Enterobacter cloacae DSM 30062 DSM is a member of Enterobacter cloacae complex Enterobacter cloacae MB11506_1 CHB is a member of Enterobacter cloacae complex Enterobacter cloacae MB_5277_05 THL is a member of Enterobacter cloacae complex Enterobacter cloacae MB_8779_05 THL is a member of Enterobacter cloacae complex Enterobacter cloacae ssp dissolvens DSM 16657T DSM is a member of Enterobacter cloacae complex Enterobacter hormaechei ssp hormaechei DSM 12409T DSM is a member of Enterobacter cloacae complex Enterobacter kobei DSM 13645T DSM is a member of Enterobacter cloacae complex Enterobacter ludwigii DSM 16688T DSM is a member of Enterobacter cloacae complex Enterobacter xiangfangensis DSM 101093T DSM is a member of Enterobacter cloacae complex Enterobacter xiangfangensis DSM 46348 DSM is a member of Enterobacter cloacae complex Escherichia coli ATCC 25922 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 25922 THL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 35218 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1103_QC DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1576 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Matching Hints table--continued on next page Report created at 2021-07-14T10:15:49 Research Use Only Page 6 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Matching Hints table--continued from previous page Matched Pattern Comment Escherichia coli DSM 30083T HAM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ESBL_EA_RSS_1528T CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli MB11464_1 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli RV412_A1_2010_06a LBK closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Klebsiella aerogenes ATCC 13048T THL synonym of Enterobacter aerogenes Klebsiella oxytoca 35130 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ATCC 700324 THL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca DSM 5175T HAM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ESBL 30298 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_1_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_2_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20879_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-07-14T10:15:49 Research Use Only Page 7 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella oxytoca VA31877_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_01 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Matching Hints table--continued on next page Report created at 2021-07-14T10:15:49 Research Use Only Page 8 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Raoultella ornithinolytica DSM 7464T DSM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MB_18887 CHB Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MHNC_19_3 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MHNC_54_1 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica UR00558_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica UR01120_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica VA10895_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella planticola DSM 4617 DSM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella planticola MHNC_28_1 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Report created at 2021-07-14T10:15:49 Research Use Only Page 9 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-07-14T10:15:49 Research Use Only Page 10 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 1 Sample Name: C7 Sample Description: Sample ID: * c 14848 Sample Creation Date/Time: 2021-07-14T09:47:46.737 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.43 562 2 (+++) Escherichia coli DSM 1576 DSM 2.40 562 3 (+++) Escherichia coli DSM 682 DSM 2.32 562 4 (+++) Escherichia coli ATCC 25922 THL 2.32 562 5 (+++) Escherichia coli DH5alpha BRL 2.28 562 6 (+++) Escherichia coli MB11464_1 CHB 2.19 562 7 (+++) Escherichia coli DSM 1103_QC DSM 2.16 562 8 (+++) Escherichia coli DSM 30083T HAM 2.15 562 9 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.11 562 10 (+++) Escherichia coli Nissl VML 2.08 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 11 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 2 Sample Name: C8 Sample Description: Sample ID: * c 14903 Sample Creation Date/Time: 2021-07-14T09:47:46.738 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.33 562 2 (+++) Escherichia coli DSM 1576 DSM 2.33 562 3 (+++) Escherichia coli ATCC 25922 THL 2.27 562 4 (+++) Escherichia coli DSM 682 DSM 2.27 562 5 (+++) Escherichia coli DH5alpha BRL 2.25 562 6 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.15 562 7 (+++) Escherichia coli DSM 1103_QC DSM 2.12 562 8 (+++) Escherichia coli MB11464_1 CHB 2.08 562 9 (+++) Escherichia coli ATCC 25922 CHB 2.06 562 10 (+++) Escherichia coli ATCC 35218 CHB 2.02 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 12 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 3 Sample Name: C9 Sample Description: Sample ID: * c 14943 Sample Creation Date/Time: 2021-07-14T09:47:46.738 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Burkholderia multivorans DSM 13243T DSM 2.11 87883 2 (+++) Burkholderia multivorans VA40721a_08 ERL 2.03 87883 3 (+) Burkholderia multivorans LMG 14293 HAM 1.99 87883 4 (+) Burkholderia multivorans VA40328_07 ERL 1.86 87883 5 (+) Burkholderia anthina LMG 16670 HAM 1.81 179879 6 (+) Burkholderia multivorans VA40721b_08 ERL 1.80 87883 7 (-) Burkholderia dolosa DSM 16088T DSM 1.66 152500 8 (-) Burkholderia cepacia_Group 18875_1 CHB 1.61 292 9 (-) Burkholderia plantarii DSM 9509T HAM 1.60 41899 10 (-) Burkholderia vietnamiensis LMG 10929T HAM 1.58 60552 Report created at 2021-07-14T10:15:49 Research Use Only Page 13 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 4 Sample Name: C10 Sample Description: Sample ID: * c 14938 Sample Creation Date/Time: 2021-07-14T09:47:46.739 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.83 574 2 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.78 72407 3 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.74 72407 4 (-) Klebsiella pneumoniae RV_BA_03_B LBK 1.68 573 5 (-) Klebsiella aerogenes ATCC 13048T THL 1.66 28451 6 (-) Klebsiella pneumoniae 37595 PFM 1.65 573 7 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.64 72407 8 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.61 574 9 (-) Klebsiella pneumoniae 37585 PFM 1.61 573 10 (-) Klebsiella variicola 150416_01 BRUS 1.44 244366 Report created at 2021-07-14T10:15:49 Research Use Only Page 14 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 5 Sample Name: C11 Sample Description: Sample ID: * c 14939 Sample Creation Date/Time: 2021-07-14T09:47:46.740 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.22 574 2 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.16 72407 3 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.09 574 4 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.05 72407 5 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.00 573 6 (+) Klebsiella variicola 150416_01 BRUS 1.98 244366 7 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.97 72407 8 (+) Klebsiella variicola 150519_01 SAH 1.96 244366 9 (+) Klebsiella variicola CC_2053 MCW 1.95 244366 10 (+) Klebsiella variicola 150519_03 SAH 1.95 244366 Report created at 2021-07-14T10:15:49 Research Use Only Page 15 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 6 Sample Name: C12 Sample Description: Sample ID: * c 14940 Sample Creation Date/Time: 2021-07-14T09:47:46.741 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.17 287 2 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.87 287 3 (+) Pseudomonas aeruginosa 8147_2 CHB 1.84 287 4 (+) Pseudomonas aeruginosa 19955_1 CHB 1.80 287 5 (-) Pseudomonas aeruginosa DSM 1117 DSM 1.62 287 6 (-) Pseudomonas aeruginosa A07_08_Pudu FLR 1.61 287 7 (-) Pseudomonas aeruginosa LMG 8029 LMG 1.55 287 8 (-) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.54 287 9 (-) Pseudomonas fragi DSM 3456T HAM 1.49 296 10 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.49 287 Report created at 2021-07-14T10:15:49 Research Use Only Page 16 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 7 Sample Name: D1 Sample Description: Sample ID: * b 13677 Sample Creation Date/Time: 2021-07-14T09:47:46.741 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.20 562 2 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.11 562 3 (+++) Escherichia coli ATCC 25922 THL 2.08 562 4 (+++) Escherichia coli DSM 682 DSM 2.02 562 5 (+++) Escherichia coli Nissl VML 2.02 562 6 (+++) Escherichia coli DSM 30083T HAM 2.01 562 7 (+++) Escherichia coli MB11464_1 CHB 2.00 562 8 (+) Escherichia coli DSM 1103_QC DSM 1.96 562 9 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.95 562 10 (+) Escherichia coli DSM 1576 DSM 1.87 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 17 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 8 Sample Name: D2 Sample Description: Sample ID: * b 13680-esco Sample Creation Date/Time: 2021-07-14T09:47:46.742 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.27 562 2 (+++) Escherichia coli DH5alpha BRL 2.22 562 3 (+++) Escherichia coli DSM 1576 DSM 2.19 562 4 (+++) Escherichia coli DSM 682 DSM 2.16 562 5 (+++) Escherichia coli ATCC 25922 THL 2.14 562 6 (+++) Escherichia coli MB11464_1 CHB 2.11 562 7 (+++) Escherichia coli ATCC 25922 CHB 2.02 562 8 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.00 562 9 (+) Escherichia coli Nissl VML 1.99 562 10 (+) Escherichia coli ATCC 35218 CHB 1.95 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 18 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 9 Sample Name: D3 Sample Description: Sample ID: * b 13680-esha Sample Creation Date/Time: 2021-07-14T09:47:46.743 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli ATCC 25922 THL 2.25 562 2 (+++) Escherichia coli DSM 30083T HAM 2.23 562 3 (+++) Escherichia coli MB11464_1 CHB 2.20 562 4 (+++) Escherichia coli DSM 682 DSM 2.19 562 5 (+++) Escherichia coli DH5alpha BRL 2.18 562 6 (+++) Escherichia coli Nissl VML 2.17 562 7 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.15 562 8 (+++) Escherichia coli DSM 1576 DSM 2.12 562 9 (+++) Escherichia coli ATCC 35218 CHB 2.07 562 10 (+++) Escherichia coli DSM 1103_QC DSM 2.05 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 19 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 10 Sample Name: D4 Sample Description: Sample ID: * b 13687-klox Sample Creation Date/Time: 2021-07-14T09:47:46.744 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca ATCC 700324 THL 2.06 571 2 (+) Raoultella ornithinolytica MB_18887 CHB 1.93 54291 3 (+) Klebsiella oxytoca VA20876_1_09 ERL 1.93 571 4 (+) Raoultella ornithinolytica DSM 7464T DSM 1.91 54291 5 (+) Klebsiella oxytoca VA20876_2_09 ERL 1.89 571 6 (+) Klebsiella oxytoca 35130 PFM 1.88 571 7 (+) Klebsiella aerogenes ATCC 13048T THL 1.88 28451 8 (+) Klebsiella oxytoca VA20879_09 ERL 1.84 571 9 (+) Raoultella ornithinolytica VA10895_09 ERL 1.78 54291 10 (+) Raoultella planticola MHNC_28_1 ERL 1.75 575 Report created at 2021-07-14T10:15:49 Research Use Only Page 20 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 11 Sample Name: D5 Sample Description: Sample ID: * b 13687-aero Sample Creation Date/Time: 2021-07-14T09:47:46.744 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca ATCC 700324 THL 2.01 571 2 (+) Klebsiella oxytoca VA20876_2_09 ERL 1.94 571 3 (+) Klebsiella oxytoca VA20879_09 ERL 1.90 571 4 (+) Klebsiella oxytoca VA20876_1_09 ERL 1.87 571 5 (+) Klebsiella oxytoca VA31877_09 ERL 1.87 571 6 (+) Klebsiella oxytoca 35130 PFM 1.84 571 7 (+) Raoultella ornithinolytica MB_18887 CHB 1.84 54291 8 (+) Klebsiella oxytoca DSM 5175T HAM 1.79 571 9 (+) Raoultella ornithinolytica UR00558_09 ERL 1.77 54291 10 (+) Raoultella ornithinolytica MHNC_54_1 ERL 1.74 54291 Report created at 2021-07-14T10:15:49 Research Use Only Page 21 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 12 Sample Name: D6 Sample Description: Sample ID: * b 13611 Sample Creation Date/Time: 2021-07-14T09:47:46.745 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter freundii DSM 30039T DSM 2.16 546 2 (+++) Citrobacter freundii 13158_2 CHB 2.15 546 3 (+++) Citrobacter braakii 9314_2 CHB 2.15 57706 4 (+++) Citrobacter freundii 22054_1 CHB 2.14 546 5 (+++) Citrobacter braakii 20663_2 CHB 2.11 57706 6 (+++) Citrobacter freundii DSM 15979 DSM 2.10 546 7 (+++) Citrobacter gillenii DSM 13694T DSM 2.07 67828 8 (+++) Citrobacter youngae DSM 17578T HAM 2.06 133448 9 (+++) Citrobacter freundii LMG 21265 LMG 2.06 546 10 (+++) Citrobacter gillenii CCUG 2749 CCUG 2.05 67828 Report created at 2021-07-14T10:15:49 Research Use Only Page 22 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 13 Sample Name: D7 Sample Description: Sample ID: * b 13615 Sample Creation Date/Time: 2021-07-14T09:47:46.746 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli ATCC 25922 THL 2.06 562 2 (+++) Escherichia coli MB11464_1 CHB 2.02 562 3 (+) Escherichia coli DH5alpha BRL 1.95 562 4 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.89 562 5 (+) Escherichia coli ATCC 35218 CHB 1.89 562 6 (+) Escherichia coli DSM 30083T HAM 1.88 562 7 (+) Escherichia coli ATCC 25922 CHB 1.84 562 8 (+) Escherichia coli DSM 1103_QC DSM 1.84 562 9 (+) Escherichia coli RV412_A1_2010_06a LBK 1.82 562 10 (+) Escherichia coli Nissl VML 1.81 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 23 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 14 Sample Name: D8 Sample Description: Sample ID: * b 13693 Sample Creation Date/Time: 2021-07-14T09:47:46.747 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.25 562 2 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.22 562 3 (+++) Escherichia coli ATCC 25922 THL 2.14 562 4 (+++) Escherichia coli MB11464_1 CHB 2.12 562 5 (+++) Escherichia coli DSM 682 DSM 2.11 562 6 (+++) Escherichia coli ATCC 35218 CHB 2.10 562 7 (+++) Escherichia coli DSM 30083T HAM 2.03 562 8 (+) Escherichia coli DSM 1576 DSM 1.99 562 9 (+) Escherichia coli DSM 1103_QC DSM 1.97 562 10 (+) Escherichia coli Nissl VML 1.95 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 24 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 15 Sample Name: D9 Sample Description: Sample ID: * b 13691 Sample Creation Date/Time: 2021-07-14T09:47:46.748 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca ATCC 700324 THL 2.02 571 2 (+++) Klebsiella oxytoca VA20876_1_09 ERL 2.00 571 3 (+) Klebsiella oxytoca VA20879_09 ERL 1.97 571 4 (+) Raoultella ornithinolytica MB_18887 CHB 1.91 54291 5 (+) Raoultella ornithinolytica DSM 7464T DSM 1.90 54291 6 (+) Raoultella ornithinolytica VA10895_09 ERL 1.88 54291 7 (+) Klebsiella oxytoca VA31877_09 ERL 1.80 571 8 (+) Raoultella ornithinolytica MHNC_54_1 ERL 1.80 54291 9 (+) Klebsiella oxytoca VA20876_2_09 ERL 1.78 571 10 (+) Raoultella ornithinolytica MHNC_19_3 ERL 1.77 54291 Report created at 2021-07-14T10:15:49 Research Use Only Page 25 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 16 Sample Name: D10 Sample Description: Sample ID: * b 13676 Sample Creation Date/Time: 2021-07-14T09:47:46.749 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter freundii 22054_1 CHB 2.12 546 2 (+++) Citrobacter braakii 20663_2 CHB 2.00 57706 3 (+++) Citrobacter youngae DSM 17578T HAM 2.00 133448 4 (+++) Citrobacter freundii DSM 15979 DSM 2.00 546 5 (+) Citrobacter freundii DSM 30039T HAM 1.99 546 6 (+) Citrobacter braakii 9314_2 CHB 1.98 57706 7 (+) Citrobacter freundii 13158_2 CHB 1.97 546 8 (+) Citrobacter freundii DSM 30039T DSM 1.97 546 9 (+) Citrobacter gillenii CCUG 2749 CCUG 1.95 67828 10 (+) Citrobacter gillenii DSM 13694T DSM 1.93 67828 Report created at 2021-07-14T10:15:49 Research Use Only Page 26 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 17 Sample Name: D11 Sample Description: Sample ID: * a 14170 Sample Creation Date/Time: 2021-07-14T09:47:46.749 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.27 72407 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.25 574 3 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.23 72407 4 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.21 573 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.18 574 6 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.04 72407 7 (+++) Klebsiella variicola 150416_01 BRUS 2.02 244366 8 (+++) Klebsiella variicola CC_2053 MCW 2.01 244366 9 (+) Klebsiella variicola 150519_03 SAH 1.97 244366 10 (+) Klebsiella variicola DSM 15968T DSM 1.92 244366 Report created at 2021-07-14T10:15:49 Research Use Only Page 27 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 18 Sample Name: D12 Sample Description: Sample ID: * a 14157 Sample Creation Date/Time: 2021-07-14T09:47:46.750 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Enterobacter asburiae DSM 17506T DSM 1.97 61645 2 (+) Enterobacter hormaechei ssp hormaechei DSM 12409T DSM 1.91 301105 3 (+) Enterobacter kobei DSM 13645T DSM 1.91 208224 4 (+) Enterobacter bugandensis DSM 101087 DSM 1.90 547 5 (+) Enterobacter cloacae DSM 30062 DSM 1.90 550 6 (+) Enterobacter bugandensis DSM 101086 DSM 1.89 547 7 (+) Enterobacter cloacae MB11506_1 CHB 1.83 550 8 (+) Enterobacter bugandensis DSM 101090 DSM 1.83 547 9 (+) Enterobacter bugandensis DSM 101092 DSM 1.82 547 10 (+) Enterobacter bugandensis DSM 101088 DSM 1.80 547 Report created at 2021-07-14T10:15:49 Research Use Only Page 28 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 19 Sample Name: E1 Sample Description: Sample ID: * a 14162 Sample Creation Date/Time: 2021-07-14T09:47:46.751 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca ATCC 700324 THL 2.11 571 2 (+) Raoultella ornithinolytica MB_18887 CHB 1.97 54291 3 (+) Klebsiella oxytoca VA20876_1_09 ERL 1.95 571 4 (+) Klebsiella oxytoca VA20876_2_09 ERL 1.94 571 5 (+) Klebsiella oxytoca 35130 PFM 1.91 571 6 (+) Klebsiella oxytoca VA20879_09 ERL 1.87 571 7 (+) Klebsiella oxytoca ESBL 30298 PFM 1.87 571 8 (+) Raoultella planticola DSM 4617 DSM 1.85 575 9 (+) Raoultella ornithinolytica VA10895_09 ERL 1.82 54291 10 (+) Raoultella ornithinolytica UR01120_09 ERL 1.80 54291 Report created at 2021-07-14T10:15:49 Research Use Only Page 29 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 20 Sample Name: E2 Sample Description: Sample ID: * a 14163 Sample Creation Date/Time: 2021-07-14T09:47:46.752 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.20 562 2 (+++) Escherichia coli MB11464_1 CHB 2.07 562 3 (+++) Escherichia coli ATCC 35218 CHB 2.06 562 4 (+++) Escherichia coli ATCC 25922 THL 2.05 562 5 (+) Escherichia coli Nissl VML 1.94 562 6 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.91 562 7 (+) Escherichia coli RV412_A1_2010_06a LBK 1.87 562 8 (+) Escherichia coli DSM 1576 DSM 1.83 562 9 (+) Escherichia coli DSM 682 DSM 1.83 562 10 (+) Escherichia coli ATCC 25922 CHB 1.83 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 30 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 21 Sample Name: E3 Sample Description: Sample ID: * a 14165 Sample Creation Date/Time: 2021-07-14T09:47:46.752 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.15 72407 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.15 573 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.12 72407 4 (+++) Klebsiella pneumoniae 37585 PFM 2.03 573 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.02 574 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.02 574 7 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.00 72407 8 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.92 39831 9 (+) Klebsiella variicola DSM 15968T HAM 1.92 244366 10 (+) Klebsiella pneumoniae 37595 PFM 1.83 573 Report created at 2021-07-14T10:15:49 Research Use Only Page 31 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 22 Sample Name: E4 Sample Description: Sample ID: * a 14166 Sample Creation Date/Time: 2021-07-14T09:47:46.753 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Burkholderia multivorans LMG 14293 HAM 2.08 87883 2 (+++) Burkholderia multivorans VA40328_07 ERL 2.01 87883 3 (+++) Burkholderia multivorans VA40721a_08 ERL 2.00 87883 4 (+) Burkholderia multivorans DSM 13243T DSM 1.98 87883 5 (+) Burkholderia dolosa DSM 16088T DSM 1.93 152500 6 (+) Burkholderia anthina LMG 16670 HAM 1.90 179879 7 (+) Burkholderia multivorans VA40721b_08 ERL 1.86 87883 8 (+) Burkholderia ambifaria LMG 11351 HAM 1.72 152480 9 (-) Burkholderia vietnamiensis LMG 10929T HAM 1.64 60552 10 (-) Burkholderia anthina DSM 16086T DSM 1.62 179879 Report created at 2021-07-14T10:15:49 Research Use Only Page 32 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 23 Sample Name: E5 Sample Description: Sample ID: * a 14156 Sample Creation Date/Time: 2021-07-14T09:47:46.754 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Burkholderia multivorans LMG 14293 HAM 2.05 87883 2 (+) Burkholderia multivorans VA40328_07 ERL 1.99 87883 3 (+) Burkholderia multivorans DSM 13243T DSM 1.91 87883 4 (+) Burkholderia dolosa DSM 16088T DSM 1.84 152500 5 (+) Burkholderia multivorans VA40721a_08 ERL 1.80 87883 6 (+) Burkholderia multivorans VA40721b_08 ERL 1.73 87883 7 (-) Burkholderia anthina LMG 16670 HAM 1.67 179879 8 (-) Burkholderia vietnamiensis LMG 10929T HAM 1.60 60552 9 (-) Burkholderia pyrrocinia LMG 14191T HAM 1.59 60550 10 (-) Burkholderia ambifaria LMG 11351 HAM 1.54 152480 Report created at 2021-07-14T10:15:49 Research Use Only Page 33 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 24 Sample Name: E6 Sample Description: Sample ID: * a 14045-klpn Sample Creation Date/Time: 2021-07-14T09:47:46.755 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) Enterobacter cloacae MB11506_1 CHB 1.56 550 2 (-) Klebsiella variicola 37924 PFM 1.56 244366 3 (-) Kosakonia cowanii DSM 18146T DSM 1.55 208223 4 (-) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.54 574 5 (-) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.53 72407 6 (-) Klebsiella pneumoniae 37585 PFM 1.48 573 7 (-) Klebsiella aerogenes ATCC 13048T THL 1.47 28451 8 (-) Escherichia coli ATCC 25922 CHB 1.46 562 9 (-) Klebsiella variicola NY_1064 MCW 1.40 244366 10 (-) Enterobacter cloacae 20105_2 CHB 1.40 550 Report created at 2021-07-14T10:15:49 Research Use Only Page 34 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 25 Sample Name: E7 Sample Description: Sample ID: * a 14045-encv Sample Creation Date/Time: 2021-07-14T09:47:46.755 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae 13159_1 CHB 2.01 550 2 (+++) Enterobacter cloacae MB11506_1 CHB 2.01 550 3 (+++) Enterobacter xiangfangensis DSM 46348 DSM 2.00 547 4 (+) Enterobacter cloacae DSM 30062 DSM 1.94 550 5 (+) Enterobacter cloacae MB_8779_05 THL 1.93 550 6 (+) Enterobacter hormaechei ssp hormaechei DSM 12409T DSM 1.92 301105 7 (+) Enterobacter xiangfangensis DSM 101093T DSM 1.91 547 8 (+) Enterobacter cloacae ssp dissolvens DSM 16657T DSM 1.84 69219 9 (+) Enterobacter cloacae MB_5277_05 THL 1.80 550 10 (+) Enterobacter kobei DSM 13645T DSM 1.80 208224 Report created at 2021-07-14T10:15:49 Research Use Only Page 35 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 26 Sample Name: E8 Sample Description: Sample ID: * a 14046-psae Sample Creation Date/Time: 2021-07-14T09:47:46.756 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.05 287 2 (+++) Pseudomonas aeruginosa 19955_1 CHB 2.05 287 3 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.93 287 4 (+) Pseudomonas aeruginosa 8147_2 CHB 1.81 287 5 (+) Pseudomonas aeruginosa DSM 1117 DSM 1.74 287 6 (-) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.65 287 7 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.57 287 8 (-) Pseudomonas aeruginosa LMG 8029 LMG 1.53 287 9 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.40 198616 10 (-) Pseudomonas citronellolis DSM 50332T HAM 1.39 53408 Report created at 2021-07-14T10:15:49 Research Use Only Page 36 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 27 Sample Name: E9 Sample Description: Sample ID: * a 14046-esco Sample Creation Date/Time: 2021-07-14T09:47:46.757 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli ATCC 25922 THL 2.26 562 2 (+++) Escherichia coli DSM 682 DSM 2.19 562 3 (+++) Escherichia coli DSM 1103_QC DSM 2.15 562 4 (+++) Escherichia coli MB11464_1 CHB 2.15 562 5 (+++) Escherichia coli DH5alpha BRL 2.12 562 6 (+++) Escherichia coli DSM 1576 DSM 2.10 562 7 (+++) Escherichia coli ATCC 25922 CHB 2.07 562 8 (+++) Escherichia coli Nissl VML 2.04 562 9 (+++) Escherichia coli DSM 30083T HAM 2.04 562 10 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.01 562 Report created at 2021-07-14T10:15:49 Research Use Only Page 37 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 28 Sample Name: E10 Sample Description: Sample ID: * a 14154 Sample Creation Date/Time: 2021-07-14T09:47:46.758 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.30 72407 2 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.27 574 3 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.23 573 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.21 72407 5 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.20 72407 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.18 574 7 (+++) Klebsiella pneumoniae 37585 PFM 2.17 573 8 (+++) Klebsiella variicola TC_4131 MCW 2.08 244366 9 (+++) Klebsiella variicola 150416_01 BRUS 2.02 244366 10 (+) Klebsiella variicola DSM 15968T DSM 1.99 244366 Report created at 2021-07-14T10:15:49 Research Use Only Page 38 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 29 Sample Name: E11 Sample Description: Sample ID: * a 14156 Sample Creation Date/Time: 2021-07-14T09:47:46.759 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter xiangfangensis DSM 101093T DSM 2.32 547 2 (+++) Enterobacter cloacae MB11506_1 CHB 2.28 550 3 (+++) Enterobacter cloacae MB_5277_05 THL 2.25 550 4 (+++) Enterobacter cloacae 13159_1 CHB 2.13 550 5 (+++) Enterobacter xiangfangensis DSM 46348 DSM 2.10 547 6 (+++) Enterobacter cloacae MB_8779_05 THL 2.05 550 7 (+) Enterobacter ludwigii DSM 16688T DSM 1.95 299767 8 (+) Enterobacter cloacae DSM 30062 DSM 1.94 550 9 (+) Enterobacter cloacae 20105_2 CHB 1.93 550 10 (+) Enterobacter bugandensis DSM 101091 DSM 1.90 547 Report created at 2021-07-14T10:15:49 Research Use Only Page 39 of 40 Run Identifier: 210714-0935-10184 Run Creation Date/Time: 2021-07-14T10:04:54.823 Sample 30 Sample Name: E12 Sample Description: Sample ID: * xxx Sample Creation Date/Time: 2021-07-14T09:47:46.760 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-07-14T10:15:49 Research Use Only Page 40 of 40