Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210715-0942-10184 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-07-15T09:48:52.283 Number of Tests: 9 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value F6 (+++) (A) * a 14088- esco (Standard) Escherichia coli 2.31 Escherichia coli 2.24 F7 (+++) (A) * a 14233-psae (Standard) Pseudomonas aeruginosa 2.30 Pseudomonas aeruginosa 2.23 F8 (+++) (A) * a 14261-klox (Standard) Klebsiella oxytoca 2.32 Klebsiella oxytoca 2.25 F9 (+++) (A) * c 14940-klpn (Standard) Klebsiella pneumoniae 2.30 Klebsiella pneumoniae 2.30 F10 (+++) (B) * c 14946-encl (Standard) Enterobacter cloacae 2.50 Enterobacter ludwigii 2.29 F11 (+++) (A) * c 15016-esma (Standard) Escherichia coli 2.30 Escherichia coli 2.26 F12 (+++) (A) * c 15064-bumu (Standard) Burkholderia multivorans 2.12 Burkholderia multivorans 2.04 Result overview table--continued on next page Report created at 2021-07-15T09:50:11 Research Use Only Page 1 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value G1 (+++) (A) * c 15066 (Standard) Burkholderia multivorans 2.31 Burkholderia multivorans 2.30 G2 (-) (C) * xxx (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-07-15T09:50:11 Research Use Only Page 2 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Matching Hints Matched Pattern Comment Burkholderia anthina DSM 16086T DSM is a member of Burkholderia cepacia complex Burkholderia anthina LMG 16670 HAM is a member of Burkholderia cepacia complex Burkholderia cenocepacia LMG 12614 HAM is a member of Burkholderia cepacia complex Burkholderia dolosa DSM 16088T DSM is a member of Burkholderia cepacia complex Burkholderia latens DSM 23436T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans DSM 13243T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans LMG 14293 HAM is a member of Burkholderia cepacia complex Burkholderia multivorans VA40328_07 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721a_08 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721b_08 ERL is a member of Burkholderia cepacia complex Burkholderia vietnamiensis LMG 10929T HAM is a member of Burkholderia cepacia complex Enterobacter asburiae DSM 17506T DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101086 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101087 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101088 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101089 DSM is a member of Enterobacter cloacae complex Matching Hints table--continued on next page Report created at 2021-07-15T09:50:11 Research Use Only Page 3 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Matching Hints table--continued from previous page Matched Pattern Comment Enterobacter bugandensis DSM 101090 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101091 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101092 DSM is a member of Enterobacter cloacae complex Enterobacter cloacae 20105_2 CHB is a member of Enterobacter cloacae complex Enterobacter ludwigii DSM 16688T DSM is a member of Enterobacter cloacae complex Escherichia coli ATCC 25922 THL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 35218 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1103_QC DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1576 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 30083T HAM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ESBL_EA_RSS_1528T CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli MB11464_1 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli RV412_A1_2010_06a LBK closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Matching Hints table--continued on next page Report created at 2021-07-15T09:50:11 Research Use Only Page 4 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella oxytoca 35130 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ATCC 700324 THL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ESBL 30298 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_1_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_2_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20879_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA31877_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Matching Hints table--continued on next page Report created at 2021-07-15T09:50:11 Research Use Only Page 5 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Raoultella ornithinolytica DSM 7464T DSM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MB_18887 CHB Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica VA10895_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Report created at 2021-07-15T09:50:11 Research Use Only Page 6 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-07-15T09:50:11 Research Use Only Page 7 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 1 Sample Name: F6 Sample Description: Sample ID: * a 14088- esco Sample Creation Date/Time: 2021-07-15T09:44:50.908 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DH5alpha BRL 2.31 562 2 (+++) Escherichia coli DSM 30083T HAM 2.24 562 3 (+++) Escherichia coli ATCC 25922 THL 2.23 562 4 (+++) Escherichia coli MB11464_1 CHB 2.22 562 5 (+++) Escherichia coli Nissl VML 2.18 562 6 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.16 562 7 (+++) Escherichia coli DSM 682 DSM 2.15 562 8 (+++) Escherichia coli ATCC 35218 CHB 2.15 562 9 (+++) Escherichia coli DSM 1576 DSM 2.13 562 10 (+++) Escherichia coli DSM 1103_QC DSM 2.12 562 Report created at 2021-07-15T09:50:11 Research Use Only Page 8 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 2 Sample Name: F7 Sample Description: Sample ID: * a 14233-psae Sample Creation Date/Time: 2021-07-15T09:44:50.908 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.30 287 2 (+++) Pseudomonas aeruginosa DSM 50071T HAM 2.23 287 3 (+++) Pseudomonas aeruginosa DSM 1117 DSM 2.18 287 4 (+++) Pseudomonas aeruginosa 8147_2 CHB 2.14 287 5 (+++) Pseudomonas aeruginosa DSM 50071T_QC DSM 2.03 287 6 (+) Pseudomonas aeruginosa 19955_1 CHB 1.97 287 7 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.91 287 8 (+) Pseudomonas aeruginosa LMG 8029 LMG 1.87 287 9 (+) Pseudomonas aeruginosa DSM 1128 DSM 1.87 287 10 (+) Pseudomonas citronellolis DSM 50332T HAM 1.70 53408 Report created at 2021-07-15T09:50:11 Research Use Only Page 9 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 3 Sample Name: F8 Sample Description: Sample ID: * a 14261-klox Sample Creation Date/Time: 2021-07-15T09:44:50.909 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca ATCC 700324 THL 2.32 571 2 (+++) Klebsiella oxytoca VA20879_09 ERL 2.25 571 3 (+++) Klebsiella oxytoca VA20876_1_09 ERL 2.22 571 4 (+++) Klebsiella oxytoca VA20876_2_09 ERL 2.12 571 5 (+++) Klebsiella oxytoca VA31877_09 ERL 2.08 571 6 (+) Klebsiella oxytoca ESBL 30298 PFM 1.99 571 7 (+) Klebsiella oxytoca 35130 PFM 1.96 571 8 (+) Raoultella ornithinolytica DSM 7464T DSM 1.91 54291 9 (+) Raoultella ornithinolytica MB_18887 CHB 1.91 54291 10 (+) Raoultella ornithinolytica VA10895_09 ERL 1.87 54291 Report created at 2021-07-15T09:50:11 Research Use Only Page 10 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 4 Sample Name: F9 Sample Description: Sample ID: * c 14940-klpn Sample Creation Date/Time: 2021-07-15T09:44:50.909 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.30 72407 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.30 573 3 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 2.17 72407 4 (+++) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 2.17 72407 5 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 2.15 574 6 (+++) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 2.15 574 7 (+++) Klebsiella pneumoniae 37585 PFM 2.07 573 8 (+++) Klebsiella pneumoniae 37595 PFM 2.02 573 9 (+++) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 2.01 39831 10 (+) Klebsiella variicola 150416_01 BRUS 1.91 244366 Report created at 2021-07-15T09:50:11 Research Use Only Page 11 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 5 Sample Name: F10 Sample Description: Sample ID: * c 14946-encl Sample Creation Date/Time: 2021-07-15T09:44:50.910 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae 20105_2 CHB 2.50 550 2 (+++) Enterobacter ludwigii DSM 16688T DSM 2.29 299767 3 (+++) Enterobacter bugandensis DSM 101090 DSM 2.17 547 4 (+++) Enterobacter bugandensis DSM 101087 DSM 2.14 547 5 (+++) Enterobacter bugandensis DSM 101086 DSM 2.12 547 6 (+++) Enterobacter bugandensis DSM 101088 DSM 2.07 547 7 (+++) Enterobacter bugandensis DSM 101091 DSM 2.07 547 8 (+++) Enterobacter bugandensis DSM 101092 DSM 2.06 547 9 (+++) Enterobacter bugandensis DSM 101089 DSM 2.06 547 10 (+++) Enterobacter asburiae DSM 17506T DSM 2.03 61645 Report created at 2021-07-15T09:50:11 Research Use Only Page 12 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 6 Sample Name: F11 Sample Description: Sample ID: * c 15016-esma Sample Creation Date/Time: 2021-07-15T09:44:50.910 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.30 562 2 (+++) Escherichia coli ATCC 35218 CHB 2.26 562 3 (+++) Escherichia coli DH5alpha BRL 2.20 562 4 (+++) Escherichia coli ATCC 25922 THL 2.20 562 5 (+++) Escherichia coli MB11464_1 CHB 2.19 562 6 (+++) Escherichia coli Nissl VML 2.18 562 7 (+++) Escherichia coli DSM 30083T HAM 2.14 562 8 (+++) Escherichia coli DSM 1576 DSM 2.01 562 9 (+) Escherichia coli DSM 682 DSM 1.99 562 10 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.96 562 Report created at 2021-07-15T09:50:11 Research Use Only Page 13 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 7 Sample Name: F12 Sample Description: Sample ID: * c 15064-bumu Sample Creation Date/Time: 2021-07-15T09:44:50.911 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Burkholderia multivorans DSM 13243T DSM 2.12 87883 2 (+++) Burkholderia multivorans LMG 14293 HAM 2.04 87883 3 (+++) Burkholderia multivorans VA40328_07 ERL 2.03 87883 4 (+) Burkholderia multivorans VA40721a_08 ERL 1.97 87883 5 (+) Burkholderia multivorans VA40721b_08 ERL 1.93 87883 6 (+) Burkholderia vietnamiensis LMG 10929T HAM 1.83 60552 7 (+) Burkholderia anthina LMG 16670 HAM 1.83 179879 8 (+) Burkholderia dolosa DSM 16088T DSM 1.74 152500 9 (+) Burkholderia latens DSM 23436T DSM 1.71 87882 10 (-) Burkholderia anthina DSM 16086T DSM 1.67 179879 Report created at 2021-07-15T09:50:11 Research Use Only Page 14 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 8 Sample Name: G1 Sample Description: Sample ID: * c 15066 Sample Creation Date/Time: 2021-07-15T09:44:50.912 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Burkholderia multivorans VA40328_07 ERL 2.31 87883 2 (+++) Burkholderia multivorans DSM 13243T DSM 2.30 87883 3 (+++) Burkholderia multivorans LMG 14293 HAM 2.27 87883 4 (+++) Burkholderia multivorans VA40721a_08 ERL 2.19 87883 5 (+++) Burkholderia multivorans VA40721b_08 ERL 2.13 87883 6 (+) Burkholderia dolosa DSM 16088T DSM 1.93 152500 7 (+) Burkholderia vietnamiensis LMG 10929T HAM 1.89 60552 8 (+) Burkholderia anthina LMG 16670 HAM 1.88 179879 9 (+) Burkholderia anthina DSM 16086T DSM 1.81 179879 10 (+) Burkholderia cenocepacia LMG 12614 HAM 1.70 95486 Report created at 2021-07-15T09:50:11 Research Use Only Page 15 of 16 Run Identifier: 210715-0942-10184 Run Creation Date/Time: 2021-07-15T09:48:52.283 Sample 9 Sample Name: G2 Sample Description: Sample ID: * xxx Sample Creation Date/Time: 2021-07-15T09:44:50.912 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-07-15T09:50:11 Research Use Only Page 16 of 16