Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210720-0945-10113 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-07-20T09:58:15.148 Number of Tests: 8 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value C8 (+++) (A) * a 14429-raor (Standard) Raoultella ornithinolytica 2.31 Raoultella ornithinolytica 2.28 C9 (+++) (A) * A 14430STAN (Standard) Staphylococcus aureus 2.16 Staphylococcus aureus 2.15 C10 (+++) (A) * C 15335 (Standard) Escherichia coli 2.05 Escherichia coli 2.05 C11 (+++) (A) * C 15336 (Standard) Klebsiella oxytoca 2.20 Klebsiella oxytoca 2.18 C12 (+++) (A) * C 15339 (Standard) Burkholderia multivorans 2.28 Burkholderia multivorans 2.18 D1 (+++) (A) * C 13351 (Standard) Escherichia coli 2.24 Escherichia coli 2.24 D2 (+++) (A) * C 15351 (Standard) Klebsiella pneumoniae 2.20 Klebsiella pneumoniae 2.03 Result overview table--continued on next page Report created at 2021-07-20T09:59:43 Research Use Only Page 1 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value D3 (-) (C) * XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-07-20T09:59:43 Research Use Only Page 2 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Matching Hints Matched Pattern Comment Burkholderia anthina LMG 16670 HAM is a member of Burkholderia cepacia complex Burkholderia cepacia_Group 18875_1 CHB is a member of Burkholderia cepacia complex Burkholderia diffusa DSM 23434T DSM is a member of Burkholderia cepacia complex Burkholderia dolosa DSM 16088T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans DSM 13243T DSM is a member of Burkholderia cepacia complex Burkholderia multivorans LMG 14293 HAM is a member of Burkholderia cepacia complex Burkholderia multivorans VA40328_07 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721a_08 ERL is a member of Burkholderia cepacia complex Burkholderia multivorans VA40721b_08 ERL is a member of Burkholderia cepacia complex Burkholderia vietnamiensis LMG 10929T HAM is a member of Burkholderia cepacia complex Escherichia coli ATCC 25922 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 25922 THL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 35218 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1103_QC DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1576 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Matching Hints table--continued on next page Report created at 2021-07-20T09:59:43 Research Use Only Page 3 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Matching Hints table--continued from previous page Matched Pattern Comment Escherichia coli DSM 30083T HAM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ESBL_EA_RSS_1528T CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli MB11464_1 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli RV412_A1_2010_06a LBK closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli W3350 MMG closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Klebsiella aerogenes ATCC 13048T THL synonym of Enterobacter aerogenes Klebsiella oxytoca 35130 PFM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca ATCC 700324 THL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_1_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20876_2_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA20879_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella oxytoca VA31877_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Matching Hints table--continued on next page Report created at 2021-07-20T09:59:43 Research Use Only Page 4 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella pneumoniae 37595 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella variicola CC_2123 MCW closely related to Klebsiella pneumoniae Raoultella ornithinolytica CCUG 52805 CCUG Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica DSM 7464T DSM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MB_18887 CHB Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MHNC_19_3 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica MHNC_46_4 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-07-20T09:59:43 Research Use Only Page 5 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Matching Hints table--continued from previous page Matched Pattern Comment Raoultella ornithinolytica MHNC_57_7 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica UR00558_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica UR01120_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella ornithinolytica VA10895_09 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella planticola DSM 3069T DSM Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Raoultella planticola MHNC_28_1 ERL Klebsiella oxytoca and species ornithinolytica / planticola / terrigena of the genus Raoultella have very similar patterns: Therefore distinguishing their species is difficult. Report created at 2021-07-20T09:59:43 Research Use Only Page 6 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-07-20T09:59:43 Research Use Only Page 7 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Sample 1 Sample Name: C8 Sample Description: Sample ID: * a 14429-raor Sample Creation Date/Time: 2021-07-20T09:53:58.242 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Raoultella ornithinolytica MB_18887 CHB 2.31 54291 2 (+++) Raoultella ornithinolytica DSM 7464T DSM 2.28 54291 3 (+++) Raoultella ornithinolytica CCUG 52805 CCUG 2.26 54291 4 (+++) Raoultella ornithinolytica MHNC_46_4 ERL 2.16 54291 5 (+++) Raoultella ornithinolytica MHNC_57_7 ERL 2.15 54291 6 (+++) Raoultella ornithinolytica MHNC_19_3 ERL 2.12 54291 7 (+++) Raoultella planticola DSM 3069T DSM 2.12 575 8 (+++) Raoultella ornithinolytica UR00558_09 ERL 2.11 54291 9 (+++) Raoultella ornithinolytica UR01120_09 ERL 2.11 54291 10 (+++) Raoultella planticola MHNC_28_1 ERL 2.10 575 Report created at 2021-07-20T09:59:43 Research Use Only Page 8 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Sample 2 Sample Name: C9 Sample Description: Sample ID: * A 14430-STAN Sample Creation Date/Time: 2021-07-20T09:53:58.242 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Staphylococcus aureus ssp aureus DSM 799 DSM 2.16 46170 2 (+++) Staphylococcus aureus ATCC 25923 THL 2.15 1280 3 (+++) Staphylococcus aureus ssp aureus DSM 20231T DSM 2.08 46170 4 (+++) Staphylococcus aureus ATCC 33591 THL 2.07 1280 5 (+++) Staphylococcus aureus ssp aureus DSM 346 DSM 2.07 46170 6 (+++) Staphylococcus aureus ssp aureus DSM 20232 DSM 2.06 46170 7 (+++) Staphylococcus aureus ssp aureus DSM 3463 DSM 2.05 46170 8 (+++) Staphylococcus aureus ATCC 29213 THL 2.03 1280 9 (+) Staphylococcus aureus ATCC 33862 THL 1.99 1280 10 (+) Staphylococcus aureus ssp aureus DSM 4910 DSM 1.96 46170 Report created at 2021-07-20T09:59:43 Research Use Only Page 9 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Sample 3 Sample Name: C10 Sample Description: Sample ID: * C 15335 Sample Creation Date/Time: 2021-07-20T09:53:58.243 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli ATCC 35218 CHB 2.05 562 2 (+++) Escherichia coli DH5alpha BRL 2.05 562 3 (+++) Escherichia coli MB11464_1 CHB 2.01 562 4 (+++) Escherichia coli DSM 30083T HAM 2.01 562 5 (+++) Escherichia coli ATCC 25922 THL 2.00 562 6 (+) Escherichia coli Nissl VML 1.99 562 7 (+) Escherichia coli RV412_A1_2010_06a LBK 1.98 562 8 (+) Escherichia coli ATCC 25922 CHB 1.89 562 9 (+) Escherichia coli DSM 682 DSM 1.85 562 10 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.85 562 Report created at 2021-07-20T09:59:43 Research Use Only Page 10 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Sample 4 Sample Name: C11 Sample Description: Sample ID: * C 15336 Sample Creation Date/Time: 2021-07-20T09:53:58.243 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella oxytoca ATCC 700324 THL 2.20 571 2 (+++) Klebsiella oxytoca VA20879_09 ERL 2.18 571 3 (+++) Klebsiella oxytoca VA20876_1_09 ERL 2.11 571 4 (+++) Raoultella ornithinolytica MB_18887 CHB 2.00 54291 5 (+) Klebsiella oxytoca 35130 PFM 1.98 571 6 (+) Klebsiella oxytoca VA31877_09 ERL 1.90 571 7 (+) Klebsiella oxytoca VA20876_2_09 ERL 1.84 571 8 (+) Raoultella ornithinolytica DSM 7464T DSM 1.79 54291 9 (+) Klebsiella aerogenes ATCC 13048T THL 1.77 28451 10 (+) Raoultella ornithinolytica VA10895_09 ERL 1.77 54291 Report created at 2021-07-20T09:59:43 Research Use Only Page 11 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Sample 5 Sample Name: C12 Sample Description: Sample ID: * C 15339 Sample Creation Date/Time: 2021-07-20T09:53:58.244 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Burkholderia multivorans VA40328_07 ERL 2.28 87883 2 (+++) Burkholderia multivorans VA40721a_08 ERL 2.18 87883 3 (+++) Burkholderia multivorans LMG 14293 HAM 2.17 87883 4 (+++) Burkholderia multivorans DSM 13243T DSM 2.13 87883 5 (+++) Burkholderia multivorans VA40721b_08 ERL 2.06 87883 6 (+) Burkholderia dolosa DSM 16088T DSM 1.95 152500 7 (+) Burkholderia vietnamiensis LMG 10929T HAM 1.89 60552 8 (+) Burkholderia anthina LMG 16670 HAM 1.79 179879 9 (+) Burkholderia diffusa DSM 23434T DSM 1.75 87882 10 (+) Burkholderia cepacia_Group 18875_1 CHB 1.70 292 Report created at 2021-07-20T09:59:43 Research Use Only Page 12 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Sample 6 Sample Name: D1 Sample Description: Sample ID: * C 13351 Sample Creation Date/Time: 2021-07-20T09:53:58.244 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 682 DSM 2.24 562 2 (+++) Escherichia coli DH5alpha BRL 2.24 562 3 (+++) Escherichia coli MB11464_1 CHB 2.24 562 4 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.21 562 5 (+++) Escherichia coli ATCC 25922 THL 2.13 562 6 (+++) Escherichia coli DSM 1576 DSM 2.10 562 7 (+++) Escherichia coli DSM 1103_QC DSM 2.10 562 8 (+++) Escherichia coli DSM 30083T HAM 2.01 562 9 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.00 562 10 (+++) Escherichia coli W3350 MMG 2.00 562 Report created at 2021-07-20T09:59:43 Research Use Only Page 13 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Sample 7 Sample Name: D2 Sample Description: Sample ID: * C 15351 Sample Creation Date/Time: 2021-07-20T09:53:58.245 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 2.20 72407 2 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.03 573 3 (+) Klebsiella pneumoniae 37585 PFM 1.87 573 4 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.85 72407 5 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T DSM 1.79 574 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.76 72407 7 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.75 574 8 (-) Klebsiella pneumoniae 37595 PFM 1.69 573 9 (-) Klebsiella variicola CC_2123 MCW 1.59 244366 10 (-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.59 574 Report created at 2021-07-20T09:59:43 Research Use Only Page 14 of 15 Run Identifier: 210720-0945-10113 Run Creation Date/Time: 2021-07-20T09:58:15.148 Sample 8 Sample Name: D3 Sample Description: Sample ID: * XXX Sample Creation Date/Time: 2021-07-20T09:53:58.245 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-07-20T09:59:43 Research Use Only Page 15 of 15