Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210722-0915-10113 Comment: Operator: maldi@FLEX-PC Run Creation Date/Time: 2021-07-22T09:23:37.035 Number of Tests: 10 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value D4 (+++) (A) * C 14434STAN (Standard) Staphylococcus aureus 2.55 Staphylococcus aureus 2.54 D5 (+++) (A) * C 14526KLVA (Standard) Klebsiella variicola 2.15 Klebsiella variicola 2.14 D6 (+++) (A) * C 14567ESCO (Standard) Escherichia coli 2.24 Escherichia coli 2.13 D7 (+++) (A) * C 14567-MILI (Standard) Enterococcus faecalis 2.31 Enterococcus faecalis 2.31 D8 (+++) (A) * C 14567BILE (Standard) Staphylococcus epidermidis 2.08 Staphylococcus epidermidis 2.06 D9 (+++) (A) * C 14567SEDE (Standard) Enterococcus faecalis 2.24 Enterococcus faecalis 2.18 Result overview table--continued on next page Report created at 2021-07-22T09:25:59 Research Use Only Page 1 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value D10 (+) (B) * C 14567MILI2 (Standard) Candida glabrata 1.76 Candida glabrata 1.74 D11 (+++) (A) * A 14483STAW (Standard) Staphylococcus aureus 2.19 Staphylococcus aureus 2.19 D12 (+++) (A) * B 14135PSAE (Standard) Pseudomonas aeruginosa 2.35 Pseudomonas aeruginosa 2.07 E1 (-) (C) * XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-07-22T09:25:59 Research Use Only Page 2 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Matching Hints Matched Pattern Comment Escherichia coli ATCC 25922 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 25922 THL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 35218 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1576 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 30083T HAM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ESBL_EA_RSS_1528T CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli MB11464_1 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli RV412_A1_2010_06a LBK closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_01 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Matching Hints table--continued on next page Report created at 2021-07-22T09:25:59 Research Use Only Page 3 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Serratia marcescens 13103_1 CHB Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Report created at 2021-07-22T09:25:59 Research Use Only Page 4 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-07-22T09:25:59 Research Use Only Page 5 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 1 Sample Name: D4 Sample Description: Sample ID: * C 14434-STAN Sample Creation Date/Time: 2021-07-22T09:19:46.150 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Staphylococcus aureus ssp aureus DSM 20231T DSM 2.55 46170 2 (+++) Staphylococcus aureus ssp aureus DSM 799 DSM 2.54 46170 3 (+++) Staphylococcus aureus ssp aureus DSM 3463 DSM 2.39 46170 4 (+++) Staphylococcus aureus ssp aureus DSM 346 DSM 2.38 46170 5 (+++) Staphylococcus aureus ATCC 33591 THL 2.35 1280 6 (+++) Staphylococcus aureus ATCC 33862 THL 2.35 1280 7 (+++) Staphylococcus aureus ssp aureus DSM 4910 DSM 2.32 46170 8 (+++) Staphylococcus aureus ATCC 29213 THL 2.32 1280 9 (+++) Staphylococcus aureus ATCC 25923 THL 2.29 1280 10 (+++) Staphylococcus aureus ssp aureus DSM 20232 DSM 2.28 46170 Report created at 2021-07-22T09:25:59 Research Use Only Page 6 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 2 Sample Name: D5 Sample Description: Sample ID: * C 14526-KLVA Sample Creation Date/Time: 2021-07-22T09:19:46.151 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola 150519_03 SAH 2.15 244366 2 (+++) Klebsiella variicola DSM 15968T DSM 2.14 244366 3 (+++) Klebsiella variicola 150416_01 BRUS 2.13 244366 4 (+++) Klebsiella variicola NY_1125 MCW 2.11 244366 5 (+++) Klebsiella variicola CC_2053 MCW 2.11 244366 6 (+++) Klebsiella variicola TC_4131 MCW 2.08 244366 7 (+++) Klebsiella variicola NY_1064 MCW 2.06 244366 8 (+++) Klebsiella variicola 37924 PFM 2.02 244366 9 (+++) Klebsiella variicola DSM 15968T HAM 2.00 244366 10 (+) Klebsiella variicola 150519_01 SAH 1.98 244366 Report created at 2021-07-22T09:25:59 Research Use Only Page 7 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 3 Sample Name: D6 Sample Description: Sample ID: * C 14567-ESCO Sample Creation Date/Time: 2021-07-22T09:19:46.151 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli ATCC 35218 CHB 2.24 562 2 (+++) Escherichia coli MB11464_1 CHB 2.13 562 3 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.11 562 4 (+++) Escherichia coli DSM 30083T HAM 2.07 562 5 (+++) Escherichia coli DH5alpha BRL 2.06 562 6 (+++) Escherichia coli ATCC 25922 THL 2.03 562 7 (+) Escherichia coli ATCC 25922 CHB 1.96 562 8 (+) Escherichia coli Nissl VML 1.96 562 9 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.93 562 10 (+) Escherichia coli DSM 1576 DSM 1.91 562 Report created at 2021-07-22T09:25:59 Research Use Only Page 8 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 4 Sample Name: D7 Sample Description: Sample ID: * C 14567-MILI Sample Creation Date/Time: 2021-07-22T09:19:46.152 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterococcus faecalis ATCC 7080 THL 2.31 1351 2 (+++) Enterococcus faecalis DSM 20409 DSM 2.31 1351 3 (+++) Enterococcus faecalis 20247_4 CHB 2.23 1351 4 (+++) Enterococcus faecalis DSM 20371 DSM 2.21 1351 5 (+++) Enterococcus faecalis DSM 20478T JUG 2.20 1351 6 (+++) Enterococcus faecalis DSM 2570 DSM 2.19 1351 7 (+++) Enterococcus faecalis 104575 LDW 2.18 1351 8 (+++) Enterococcus faecalis ATCC 29212 CHB 2.18 1351 9 (+++) Enterococcus faecalis DSM 6134 DSM 2.14 1351 10 (+) Enterococcus faecalis 105652 LDW 1.88 1351 Report created at 2021-07-22T09:25:59 Research Use Only Page 9 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 5 Sample Name: D8 Sample Description: Sample ID: * C 14567-BILE Sample Creation Date/Time: 2021-07-22T09:19:46.152 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Staphylococcus epidermidis 6b_s ESL 2.08 1282 2 (+++) Staphylococcus epidermidis DSM 1798 DSM 2.06 1282 3 (+) Staphylococcus epidermidis DSM 3269 DSM 1.93 1282 4 (+) Staphylococcus epidermidis DSM 4851 DSM 1.91 1282 5 (+) Staphylococcus epidermidis 10547 CHB 1.86 1282 6 (+) Staphylococcus epidermidis 4b_r ESL 1.86 1282 7 (+) Staphylococcus epidermidis ATCC 12228 THL 1.79 1282 8 (-) Staphylococcus epidermidis ATCC 14990T THL 1.58 1282 9 (-) Staphylococcus epidermidis ATCC 12228 CHB 1.52 1282 10 (-) Serratia marcescens 13103_1 CHB 1.43 615 Report created at 2021-07-22T09:25:59 Research Use Only Page 10 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 6 Sample Name: D9 Sample Description: Sample ID: * C 14567-SEDE Sample Creation Date/Time: 2021-07-22T09:19:46.153 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterococcus faecalis ATCC 7080 THL 2.24 1351 2 (+++) Enterococcus faecalis DSM 20409 DSM 2.18 1351 3 (+++) Enterococcus faecalis 20247_4 CHB 2.16 1351 4 (+++) Enterococcus faecalis ATCC 29212 CHB 2.14 1351 5 (+++) Enterococcus faecalis DSM 20371 DSM 2.13 1351 6 (+++) Enterococcus faecalis DSM 20478T JUG 2.13 1351 7 (+++) Enterococcus faecalis DSM 2570 DSM 2.11 1351 8 (+++) Enterococcus faecalis 104575 LDW 2.08 1351 9 (+++) Enterococcus faecalis DSM 6134 DSM 2.03 1351 10 (+) Enterococcus faecalis 105652 LDW 1.76 1351 Report created at 2021-07-22T09:25:59 Research Use Only Page 11 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 7 Sample Name: D10 Sample Description: Sample ID: * C 14567-MILI2 Sample Creation Date/Time: 2021-07-22T09:19:46.153 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Candida glabrata CBS 2663 CBS 1.76 5478 2 (+) Candida glabrata CBS 138T CBS 1.74 284593 3 (+) Candida glabrata CBS 8947 CBS 1.73 5478 4 (+) Candida glabrata 10035463_101 USH 1.72 5478 5 (-) Candida glabrata ATCC 90030 VML 1.68 5478 6 (-) Candida glabrata CBS 2175 CBS 1.68 5478 7 (-) Candida glabrata CBS 858 CBS 1.65 5478 8 (-) Candida glabrata CBS 862 CBS 1.64 5478 9 (-) Candida glabrata CBS 863 CBS 1.62 5478 10 (-) Candida glabrata CBS 860 CBS 1.57 5478 Report created at 2021-07-22T09:25:59 Research Use Only Page 12 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 8 Sample Name: D11 Sample Description: Sample ID: * A 14483-STAW Sample Creation Date/Time: 2021-07-22T09:19:46.154 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Staphylococcus aureus ssp aureus DSM 3463 DSM 2.19 46170 2 (+++) Staphylococcus aureus ssp aureus DSM 799 DSM 2.19 46170 3 (+++) Staphylococcus aureus ATCC 33862 THL 2.18 1280 4 (+++) Staphylococcus aureus ssp aureus DSM 20231T DSM 2.11 46170 5 (+++) Staphylococcus aureus ssp aureus DSM 4910 DSM 2.08 46170 6 (+++) Staphylococcus aureus ATCC 33591 THL 2.08 1280 7 (+++) Staphylococcus aureus ssp aureus DSM 346 DSM 2.04 46170 8 (+++) Staphylococcus aureus ATCC 29213 THL 2.00 1280 9 (+) Staphylococcus aureus ssp aureus DSM 11822 DSM 1.98 46170 10 (+) Staphylococcus aureus ATCC 25923 THL 1.98 1280 Report created at 2021-07-22T09:25:59 Research Use Only Page 13 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 9 Sample Name: D12 Sample Description: Sample ID: * B 14135-PSAE Sample Creation Date/Time: 2021-07-22T09:19:46.154 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.35 287 2 (+++) Pseudomonas aeruginosa DSM 50071T_QC DSM 2.07 287 3 (+++) Pseudomonas aeruginosa DSM 50071T HAM 2.05 287 4 (+) Pseudomonas aeruginosa DSM 1117 DSM 1.99 287 5 (+) Pseudomonas aeruginosa 8147_2 CHB 1.97 287 6 (+) Pseudomonas aeruginosa 19955_1 CHB 1.95 287 7 (+) Pseudomonas aeruginosa LMG 8029 LMG 1.82 287 8 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.74 287 9 (+) Pseudomonas aeruginosa DSM 1128 DSM 1.73 287 10 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.57 198616 Report created at 2021-07-22T09:25:59 Research Use Only Page 14 of 15 Run Identifier: 210722-0915-10113 Run Creation Date/Time: 2021-07-22T09:23:37.035 Sample 10 Sample Name: E1 Sample Description: Sample ID: * XXX Sample Creation Date/Time: 2021-07-22T09:19:46.155 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-07-22T09:25:59 Research Use Only Page 15 of 15