Bruker MALDI Biotyper 
Identification Results 


Run Info: 
Run Identifier: 210726-1055-10113 
Comment: 
Operator: maldi@FLEX-PC 
Run Creation Date/Time: 2021-07-26T11:11:59.849 
Number of Tests: 9 
Type: Standard 
BTS-QC: not present 
BTS-QC Position: 
Instrument ID: 269944.01064 
Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 
Result Overview 

Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
E10 
(+++) (A) 
* c 15800 
(Standard) Pseudomonas aeruginosa 2.28 Pseudomonas aeruginosa 2.11 
E11 
(+++) (A) 
* c 15414 
(Standard) Acinetobacter baumannii 2.20 Acinetobacter baumannii 2.17 
E12 
(+++) (A) 
* c 15448-21 
(Standard) Pseudomonas aeruginosa 2.05 Pseudomonas aeruginosa 1.81 
F1 
(+) (B) 
* c 15448-22 
(Standard) Pseudomonas aeruginosa 1.81 Pseudomonas aeruginosa 1.78 
F2 
(+++) (A) 
* c 15645 
(Standard) Escherichia coli 2.37 Escherichia coli 2.35 
F3 
(+) (B) 
* c 15685-bile 
(Standard) Staphylococcus haemolyticus 1.83 Staphylococcus haemolyticus 1.74 
F4 
(+++) (A) 
* a 14502 
(Standard) Serratia marcescens 2.02 Serratia marcescens 2.00 
Result overview table--continued on next page 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 

Result overview table--continued from previous page 
Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
F5 
(+) (B) 
* c 15685-pruhl 
(Standard) Enterococcus faecalis 1.84 Enterococcus faecalis 1.74 
F6 
(-) (C) 
* xsxx 
(Standard) no peaks found 0.00 no peaks found 0.00 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 

Matching Hints 

Matched Pattern Comment 
Acinetobacter baumannii 
13101_1 CHB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii DSM 
30007T HAM 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii DSM 
30008 DSM 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii DSM 
30011 DSM 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii ECI 
RUH_875 HCB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii ECII 
RUH_134 HCB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii ECIII 
RUH_5875 HCB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii 
GEIH_2000 HCB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter baumannii LMG 
994 HAM 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Acinetobacter nosocomialis 
AL_95 HCB 
Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must 
be performed to permit reliable species identification. 
Burkholderia multivorans LMG 
14293 HAM is a member of Burkholderia cepacia complex 
Burkholderia multivorans 
VA40328_07 ERL is a member of Burkholderia cepacia complex 
Escherichia coli ATCC 25922 
THL 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1103_QC 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1576 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Matching Hints table--continued on next page 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Escherichia coli DSM 30083T 
HAM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
ESBL_EA_RSS_1528T CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli MB11464_1 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
RV412_A1_2010_06a LBK 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Serratia marcescens (PX) 
24086109 MLD 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens 13103_1 
CHB 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens DSM 12481 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens DSM 12483 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens DSM 12485 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens DSM 30122 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens ssp 
marcescens DSM 30121T DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia marcescens ssp 
sakuensis CIP 107489T HAM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 
Serratia ureilytica DSM 16952T 
DSM 
Species marcescens / ureilytica of the genus Serratia have very similar patterns: 
Therefore distinguishing their species is difficult. 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 

Meaning of Score Values 

Range Interpretation Symbols Color 
2.00 - 3.00 High-confidence identification (+++) green 
1.70 - 1.99 Low-confidence identification (+) yellow 
0.00 - 1.69 No Organism Identification Possible (-) red 

Meaning of Consistency Categories (A - C) 

Category Interpretation 
(A) 
High consistency: The best match is a high-confidence identification. The second-best match is (1) a 
high-confidence identification in which the species is identical to the best match, (2) a low-confidence 
identification in which the species or genus is identical to the best match, or (3) a non-identification. 
(B) 
Low consistency: The requirements for high consistency are not met. The best match is a high- or low-
confidence identification. The second-best match is (1) a high- or low-confidence identification in 
which the genus is identical to the best match or (2) a non-identification. 
(C) No consistency: The requirements for high or low consistency are not met. 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 


Sample 1 

Sample Name: E10 
Sample Description: 
Sample ID: * c 15800 
Sample Creation Date/Time: 2021-07-26T11:07:23.023 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Pseudomonas aeruginosa ATCC 27853 THL 2.28 287 
2 
(+++) Pseudomonas aeruginosa DSM 1117 DSM 2.11 287 
3 
(+++) Pseudomonas aeruginosa DSM 50071T HAM 2.10 287 
4 
(+++) Pseudomonas aeruginosa 8147_2 CHB 2.08 287 
5 
(+++) Pseudomonas aeruginosa DSM 50071T_QC DSM 2.01 287 
6 
(+) Pseudomonas aeruginosa LMG 8029 LMG 1.97 287 
7 
(+) Pseudomonas aeruginosa 19955_1 CHB 1.96 287 
8 
(+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.83 287 
9 
(+) Pseudomonas jinjuensis LMG 21316T HAM 1.76 198616 
10 
(+) Pseudomonas aeruginosa DSM 1128 DSM 1.73 287 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 


Sample 2 

Sample Name: E11 
Sample Description: 
Sample ID: * c 15414 
Sample Creation Date/Time: 2021-07-26T11:07:23.024 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Acinetobacter baumannii LMG 994 HAM 2.20 470 
2 
(+++) Acinetobacter baumannii ECII RUH_134 HCB 2.17 470 
3 
(+++) Acinetobacter baumannii GEIH_2000 HCB 2.13 470 
4 
(+++) Acinetobacter baumannii ECIII RUH_5875 HCB 2.12 470 
5 
(+++) Acinetobacter baumannii DSM 30011 DSM 2.06 470 
6 
(+++) Acinetobacter baumannii ECI RUH_875 HCB 2.03 470 
7 
(+) Acinetobacter baumannii DSM 30007T HAM 1.99 470 
8 
(+) Acinetobacter baumannii DSM 30008 DSM 1.91 470 
9 
(+) Acinetobacter baumannii 13101_1 CHB 1.86 470 
10 
(-) Acinetobacter nosocomialis AL_95 HCB 1.61 72607 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 


Sample 3 

Sample Name: E12 
Sample Description: 
Sample ID: * c 15448-21 
Sample Creation Date/Time: 2021-07-26T11:07:23.024 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Pseudomonas aeruginosa ATCC 27853 THL 2.05 287 
2 
(+) Pseudomonas aeruginosa 19955_1 CHB 1.81 287 
3 
(+) Pseudomonas aeruginosa DSM 50071T HAM 1.77 287 
4 
(+) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.73 287 
5 
(+) Pseudomonas aeruginosa 8147_2 CHB 1.70 287 
6 
(-) Pseudomonas aeruginosa LMG 8029 LMG 1.52 287 
7 
(-) Pseudomonas aeruginosa DSM 1117 DSM 1.50 287 
8 
(-) Pseudomonas aeruginosa DSM 1128 DSM 1.46 287 
9 
(-) Pseudomonas jinjuensis LMG 21316T HAM 1.35 198616 
10 
(-) Pseudomonas aeruginosa A07_08_Pudu FLR 1.34 287 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 


Sample 4 

Sample Name: F1 
Sample Description: 
Sample ID: * c 15448-22 
Sample Creation Date/Time: 2021-07-26T11:07:23.025 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based B 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+) Pseudomonas aeruginosa 8147_2 CHB 1.81 287 
2 
(+) Pseudomonas aeruginosa ATCC 27853 THL 1.78 287 
3 
(+) Pseudomonas aeruginosa 19955_1 CHB 1.77 287 
4 
(-) Pseudomonas aeruginosa DSM 50071T HAM 1.61 287 
5 
(-) Dietzia natronolimnaea 091216_01 LSM 1.38 161920 
6 
(-) Prevotella heparinolytica ENR_0211 ENR 1.34 28113 
7 
(-) Lactobacillus satsumensis DSM 16230T DSM 1.33 259059 
8 
(-) Pseudomonas aeruginosa LMG 8029 LMG 1.31 287 
9 
(-) Pseudomonas aeruginosa DSM 1117 DSM 1.30 287 
10 
(-) Pseudomonas aeruginosa A07_08_Pudu FLR 1.28 287 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 


Sample 5 

Sample Name: F2 
Sample Description: 
Sample ID: * c 15645 
Sample Creation Date/Time: 2021-07-26T11:07:23.025 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli RV412_A1_2010_06a LBK 2.37 562 
2 
(+++) Escherichia coli DH5alpha BRL 2.35 562 
3 
(+++) Escherichia coli DSM 682 DSM 2.30 562 
4 
(+++) Escherichia coli DSM 1576 DSM 2.26 562 
5 
(+++) Escherichia coli ATCC 25922 THL 2.12 562 
6 
(+++) Escherichia coli MB11464_1 CHB 2.08 562 
7 
(+++) Escherichia coli Nissl VML 2.05 562 
8 
(+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.04 562 
9 
(+++) Escherichia coli DSM 30083T HAM 2.02 562 
10 
(+++) Escherichia coli DSM 1103_QC DSM 2.00 562 

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Run Identifier: 210726-1055-10113 Run Creation Date/Time: 2021-07-26T11:11:59.849 


Sample 6 

Sample Name: F3 
Sample Description: 
Sample ID: * c 15685-bile 
Sample Creation Date/Time: 2021-07-26T11:07:23.026 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based B 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+) Staphylococcus haemolyticus DSM 20264 DSM 1.83 1283 
2 
(+) Staphylococcus haemolyticus DSM 20228 BRB 1.74 1283 
3 
(+) Staphylococcus haemolyticus 10024 CHB 1.70 1283 
4 
(-) Staphylococcus haemolyticus DSM 20228 DSM 1.69 1283 
5 
(-) Staphylococcus haemolyticus 19 ESL 1.61 1283 
6 
(-) Staphylococcus haemolyticus DSM 20263T DSM 1.53 1283 
7 
(-) Staphylococcus haemolyticus DSM 20264 BRB 1.50 1283 
8 
(-) Staphylococcus capitis ssp capitis DSM 20325 DSM 1.45 72758 
9 
(-) Staphylococcus simulans DSM 20323 DSM 1.45 1286 
10 
(-) Staphylococcus haemolyticus Mb18803_2 CHB 1.43 1283 

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Sample 7 

Sample Name: F4 
Sample Description: 
Sample ID: * a 14502 
Sample Creation Date/Time: 2021-07-26T11:07:23.026 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Serratia marcescens 13103_1 CHB 2.02 615 
2 
(+++) Serratia marcescens (PX) 24086109 MLD 2.00 615 
3 
(+) Serratia marcescens ssp marcescens DSM 30121T DSM 1.86 211759 
4 
(+) Serratia marcescens DSM 12485 DSM 1.83 615 
5 
(+) Serratia marcescens ssp sakuensis CIP 107489T HAM 1.82 211760 
6 
(-) Serratia marcescens DSM 30122 DSM 1.56 615 
7 
(-) Serratia marcescens DSM 12481 DSM 1.50 615 
8 
(-) Serratia marcescens DSM 12483 DSM 1.49 615 
9 
(-) Serratia ureilytica DSM 16952T DSM 1.48 300181 
10 
(-) Paraburkholderia fungorum LMG 20227T HAM 1.44 134537 

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Sample 8 

Sample Name: F5 
Sample Description: 
Sample ID: * c 15685-pruhl 
Sample Creation Date/Time: 2021-07-26T11:07:23.027 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based B 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+) Enterococcus faecalis ATCC 7080 THL 1.84 1351 
2 
(+) Enterococcus faecalis 20247_4 CHB 1.74 1351 
3 
(-) Enterococcus faecalis ATCC 29212 CHB 1.69 1351 
4 
(-) Burkholderia multivorans LMG 14293 HAM 1.61 87883 
5 
(-) Enterococcus faecalis DSM 20478T JUG 1.54 1351 
6 
(-) Enterococcus faecalis 104575 LDW 1.52 1351 
7 
(-) Enterococcus faecalis DSM 20409 DSM 1.50 1351 
8 
(-) Burkholderia multivorans VA40328_07 ERL 1.48 87883 
9 
(-) Enterococcus faecalis DSM 2570 DSM 1.45 1351 
10 
(-) Enterococcus faecalis DSM 20371 DSM 1.40 1351 

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Sample 9 

Sample Name: F6 
Sample Description: 
Sample ID: * xsxx 
Sample Creation Date/Time: 2021-07-26T11:07:23.027 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based C 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(-) no peaks found 0.00 -
2 
(-) no peaks found 0.00 -
3 
(-) no peaks found 0.00 -
4 
(-) no peaks found 0.00 -
5 
(-) no peaks found 0.00 -
6 
(-) no peaks found 0.00 -
7 
(-) no peaks found 0.00 -
8 
(-) no peaks found 0.00 -
9 
(-) no peaks found 0.00 -
10 
(-) no peaks found 0.00 -

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