Bruker MALDI Biotyper 
Identification Results 


Run Info: 
Run Identifier: 210802-0948-10113 
Comment: 
Operator: maldi@FLEX-PC 
Run Creation Date/Time: 2021-08-02T09:53:05.695 
Number of Tests: 6 
Type: Standard 
BTS-QC: not present 
BTS-QC Position: 
Instrument ID: 269944.01064 
Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 

Result Overview 

Sample 
Name Sample ID Organism (best match) Score 
Value 
Organism (second-best 
match) 
Score 
Value 
G3 
(+++) (A) 
* c 16177 
(Standard) Escherichia coli 2.36 Escherichia coli 2.33 
G4 
(+) (B) 
* c 16192 
(Standard) Escherichia coli 1.89 Escherichia coli 1.86 
G5 
(+) (B) 
* a 14947 bila 
(Standard) Candida albicans 1.90 Candida albicans 1.82 
G6 
(-) (C) 
* a 14947-seda 
(Standard) 
No Organism Identification 
Possible 1.68 No Organism Identification 
Possible 1.63 
G7 
(+++) (A) 
* c 16118 
(Standard) Klebsiella pneumoniae 2.00 Klebsiella pneumoniae 1.98 
G8 
(-) (C) 
* xxx 
(Standard) no peaks found 0.00 no peaks found 0.00 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 

Matching Hints 

Matched Pattern Comment 
Escherichia coli ATCC 25922 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 25922 
THL 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli ATCC 35218 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli B421 UFL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 1576 
DSM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 30083T 
HAM 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli MB11464_1 
CHB 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Escherichia coli 
RV412_A1_2010_06a LBK 
closely related to Shigella / Escherichia fergusonii and not definitely 
distinguishable at the moment 
Klebsiella pneumoniae 37585 
PFM closely related to Klebsiella variicola 
Klebsiella pneumoniae 
RV_BA_03_B LBK closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
ozaenae DSM 16358T HAM closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae 9295_1 CHB closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T 
HAM 
closely related to Klebsiella variicola 
Matching Hints table--continued on next page 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 

Matching Hints table--continued from previous page 
Matched Pattern Comment 
Klebsiella pneumoniae ssp 
pneumoniae DSM 30104T_QC 
DSM 
closely related to Klebsiella variicola 
Klebsiella pneumoniae ssp 
rhinoscleromatis DSM 16231T 
HAM 
closely related to Klebsiella variicola 
Klebsiella variicola DSM 
15968T HAM closely related to Klebsiella pneumoniae 
Klebsiella variicola NY_1036 
MCW closely related to Klebsiella pneumoniae 
Klebsiella variicola NY_1125 
MCW closely related to Klebsiella pneumoniae 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 

Meaning of Score Values 

Range Interpretation Symbols Color 
2.00 - 3.00 High-confidence identification (+++) green 
1.70 - 1.99 Low-confidence identification (+) yellow 
0.00 - 1.69 No Organism Identification Possible (-) red 

Meaning of Consistency Categories (A - C) 

Category Interpretation 
(A) 
High consistency: The best match is a high-confidence identification. The second-best match is (1) a 
high-confidence identification in which the species is identical to the best match, (2) a low-confidence 
identification in which the species or genus is identical to the best match, or (3) a non-identification. 
(B) 
Low consistency: The requirements for high consistency are not met. The best match is a high- or low-
confidence identification. The second-best match is (1) a high- or low-confidence identification in 
which the genus is identical to the best match or (2) a non-identification. 
(C) No consistency: The requirements for high or low consistency are not met. 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 


Sample 1 

Sample Name: G3 
Sample Description: 
Sample ID: * c 16177 
Sample Creation Date/Time: 2021-08-02T09:50:47.934 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Escherichia coli ATCC 35218 CHB 2.36 562 
2 
(+++) Escherichia coli MB11464_1 CHB 2.33 562 
3 
(+++) Escherichia coli DSM 30083T HAM 2.25 562 
4 
(+++) Escherichia coli RV412_A1_2010_06a LBK 2.23 562 
5 
(+++) Escherichia coli Nissl VML 2.18 562 
6 
(+++) Escherichia coli DH5alpha BRL 2.18 562 
7 
(+++) Escherichia coli ATCC 25922 THL 2.18 562 
8 
(+++) Escherichia coli B421 UFL 2.09 562 
9 
(+) Escherichia coli ATCC 25922 CHB 1.99 562 
10 
(+) Escherichia coli DSM 1576 DSM 1.98 562 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 


Sample 2 

Sample Name: G4 
Sample Description: 
Sample ID: * c 16192 
Sample Creation Date/Time: 2021-08-02T09:50:47.935 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based B 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+) Escherichia coli ATCC 35218 CHB 1.89 562 
2 
(+) Escherichia coli DH5alpha BRL 1.86 562 
3 
(+) Escherichia coli MB11464_1 CHB 1.74 562 
4 
(-) Escherichia coli RV412_A1_2010_06a LBK 1.63 562 
5 
(-) Escherichia coli ATCC 25922 THL 1.60 562 
6 
(-) Escherichia coli Nissl VML 1.57 562 
7 
(-) Escherichia coli DSM 682 DSM 1.54 562 
8 
(-) Escherichia coli DSM 30083T HAM 1.51 562 
9 
(-) Escherichia coli ATCC 25922 CHB 1.49 562 
10 
(-) Escherichia coli DSM 1576 DSM 1.47 562 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 


Sample 3 

Sample Name: G5 
Sample Description: 
Sample ID: * a 14947 bila 
Sample Creation Date/Time: 2021-08-02T09:50:47.936 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based B 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+) Candida albicans DSM 6659 DSM 1.90 5476 
2 
(+) Candida albicans DSM 1665 DSM 1.82 5476 
3 
(+) Candida albicans CBS 562 NT CBS 1.77 5476 
4 
(+) Candida albicans CBS 5145 CBS 1.76 5476 
5 
(+) Candida albicans CBS 8190 CBS 1.74 5476 
6 
(+) Candida albicans VA_17248_07 04 UKE 1.72 5476 
7 
(+) Candida albicans ATCC 10231 THL 1.71 5476 
8 
(-) Candida albicans_(africana) CBS 8781 CBS 1.63 241526 
9 
(-) Candida albicans CBS 2692 CBS 1.60 5476 
10 
(-) Candida albicans CBS 2690 CBS 1.59 5476 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 


Sample 4 

Sample Name: G6 
Sample Description: 
Sample ID: * a 14947-seda 
Sample Creation Date/Time: 2021-08-02T09:50:47.936 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based C 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(-) Candida krusei CBS 2457 CBS 1.68 4909 
2 
(-) Candida krusei CBS 6521 CBS 1.63 4909 
3 
(-) Candida krusei CBS 2107 CBS 1.63 4909 
4 
(-) Candida krusei CBS 6520 CBS 1.61 4909 
5 
(-) Candida krusei CBS 6799 CBS 1.56 4909 
6 
(-) Candida krusei ATCC 14243 THL 1.55 4909 
7 
(-) Candida krusei RV491_Sep09_D LBK 1.51 4909 
8 
(-) Candida krusei CBS 5147T CBS 1.47 4909 
9 
(-) Candida krusei CBS 5217 CBS 1.35 4909 
10 
(-) Candida krusei 36 PSB 1.18 4909 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 


Sample 5 

Sample Name: G7 
Sample Description: 
Sample ID: * c 16118 
Sample Creation Date/Time: 2021-08-02T09:50:47.937 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based A 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.00 573 
2 
(+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.98 574 
3 
(+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.97 39831 
4 
(+) Klebsiella variicola DSM 15968T HAM 1.94 244366 
5 
(+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.91 72407 
6 
(+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.90 72407 
7 
(+) Klebsiella variicola NY_1036 MCW 1.89 244366 
8 
(+) Klebsiella pneumoniae 37585 PFM 1.85 573 
9 
(+) Klebsiella variicola NY_1125 MCW 1.82 244366 
10 
(+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.81 72407 

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Run Identifier: 210802-0948-10113 Run Creation Date/Time: 2021-08-02T09:53:05.695 


Sample 6 

Sample Name: G8 
Sample Description: 
Sample ID: * xxx 
Sample Creation Date/Time: 2021-08-02T09:50:47.937 
Sample Type: Standard 
Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 
Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 
ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par 
AutoXecute Method: MBT_AutoX 
Consistency Category (based C 

on 2 best matches): 
Applied Taxonomy Tree: Bruker Taxonomy 

Rank 
(Quality) Matched Pattern Score 
Value NCBI Identifier 
1 
(-) no peaks found 0.00 -
2 
(-) no peaks found 0.00 -
3 
(-) no peaks found 0.00 -
4 
(-) no peaks found 0.00 -
5 
(-) no peaks found 0.00 -
6 
(-) no peaks found 0.00 -
7 
(-) no peaks found 0.00 -
8 
(-) no peaks found 0.00 -
9 
(-) no peaks found 0.00 -
10 
(-) no peaks found 0.00 -

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