Bruker MALDI Biotyper Identification Results Run Info: Run Identifier: 210809-1051-10184 Comment: Operator: MALDI@FLEX-PC Run Creation Date/Time: 2021-08-09T12:20:23.005 Number of Tests: 29 Type: Standard BTS-QC: not present BTS-QC Position: Instrument ID: 269944.01064 Server Version: 4.1.100 (PYTH) 174 2019-06-158_01-16-09 Result Overview Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value E1 (+) (B) A 15166 (Standard) Klebsiella pneumoniae 1.91 Klebsiella pneumoniae 1.85 E2 (+++) (A) A 15246 (Standard) Escherichia coli 2.42 Escherichia coli 2.38 E3 (+++) (A) A 15247 (Standard) Klebsiella pneumoniae 2.00 Klebsiella pneumoniae 1.98 E4 (+++) (B) C 16508-1 (Standard) Enterobacter cloacae 2.19 Enterobacter xiangfangensis 2.12 E5 (+++) (B) C 16508-2 (Standard) Enterobacter cloacae 2.18 Enterobacter xiangfangensis 2.18 E6 (+) (B) C 16499 (Standard) Klebsiella pneumoniae 1.96 Klebsiella pneumoniae 1.86 E7 (+) (B) C 16498-KLPN (Standard) Klebsiella pneumoniae 1.87 Klebsiella pneumoniae 1.83 Result overview table--continued on next page Report created at 2021-08-09T12:26:41 Research Use Only Page 1 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value E8 (+++) (A) C 16498-ESCO (Standard) Escherichia coli 2.35 Escherichia coli 2.32 E9 (+++) (C) C 16485 (Standard) Klebsiella variicola 2.00 Klebsiella variicola 1.99 E10 (+) (B) C 16482 (Standard) Serratia marcescens 1.95 Serratia marcescens 1.87 E11 (+) (C) C 16457 (Standard) Klebsiella pneumoniae 1.91 Klebsiella pneumoniae 1.89 E12 (+) (C) C 16485 (Standard) Pseudomonas aeruginosa 1.95 Pseudomonas aeruginosa 1.80 F1 (+) (C) C 16457 (Standard) Pseudomonas aeruginosa 1.85 Pseudomonas aeruginosa 1.79 F2 (+++) (A) A 15295 (Standard) Escherichia coli 2.34 Escherichia coli 2.31 F3 (+++) (A) A 15319-ESMA (Standard) Escherichia coli 2.10 Escherichia coli 2.08 F4 (+) (B) A 15319 (Standard) Enterobacter cloacae 1.94 Enterobacter cloacae 1.88 F5 (+++) (A) A 15329 (Standard) Escherichia coli 2.07 Escherichia coli 1.97 F6 (+) (B) B 15405-KLOX (Standard) Klebsiella pneumoniae 1.97 Klebsiella pneumoniae 1.95 F7 (+++) (A) B 15405-CIFR (Standard) Citrobacter freundii 2.30 Citrobacter freundii 2.21 F8 (+++) (C) C 15614 (Standard) Serratia marcescens 2.12 Serratia marcescens 1.99 F9 (+++) (A) C 15644 (Standard) Enterobacter cloacae 2.07 Enterobacter cloacae 1.99 F10 (+++) (B) C 16659-1 (Standard) Enterobacter cloacae 2.24 Enterobacter xiangfangensis 2.23 F11 (+++) (B) C 16659-2 (Standard) Enterobacter cloacae 2.26 Enterobacter ludwigii 2.16 Result overview table--continued on next page Report created at 2021-08-09T12:26:41 Research Use Only Page 2 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Result overview table--continued from previous page Sample Name Sample ID Organism (best match) Score Value Organism (second-best match) Score Value F12 (+++) (B) C 16661-OT (Standard) Enterobacter cloacae 2.24 Enterobacter ludwigii 2.09 G1 (+++) (A) B 15405-1 (Standard) Pseudomonas aeruginosa 2.05 Pseudomonas aeruginosa 1.94 G2 (+++) (A) B 15406 (Standard) Pseudomonas aeruginosa 2.36 Pseudomonas aeruginosa 2.24 G3 (+++) (C) C 15614 (Standard) Pseudomonas aeruginosa 2.09 Pseudomonas aeruginosa 1.94 G4 (+++) (A) B 15405-2 (Standard) Pseudomonas aeruginosa 2.14 Pseudomonas aeruginosa 2.12 G5 (-) (C) C XXX (Standard) no peaks found 0.00 no peaks found 0.00 Report created at 2021-08-09T12:26:41 Research Use Only Page 3 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Matching Hints Matched Pattern Comment Acinetobacter baumannii B389 UFL Member of the Acinetobacter baumannii /calcoaceticus complex. Extraction must be performed to permit reliable species identification. Citrobacter braakii 9314_2 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii 13158_2 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii 22054_1 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii DSM 15979 DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii DSM 30039T DSM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii DSM 30039T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii LMG 21265 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter freundii LMG 3251 LMG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter gillenii CCUG 2749 CCUG Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter koseri Mu15167_1 CHB Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Citrobacter youngae DSM 17578T HAM Species of this genus have very similar patterns: Therefore distinguishing their species is difficult. Enterobacter asburiae CCM 4032 CCM is a member of Enterobacter cloacae complex Enterobacter asburiae DSM 17506T DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101086 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101087 DSM is a member of Enterobacter cloacae complex Matching Hints table--continued on next page Report created at 2021-08-09T12:26:41 Research Use Only Page 4 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Matching Hints table--continued from previous page Matched Pattern Comment Enterobacter bugandensis DSM 101088 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101089 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101090 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101091 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 101092 DSM is a member of Enterobacter cloacae complex Enterobacter bugandensis DSM 29888T DSM is a member of Enterobacter cloacae complex Enterobacter cloacae 13159_1 CHB is a member of Enterobacter cloacae complex Enterobacter cloacae 20105_2 CHB is a member of Enterobacter cloacae complex Enterobacter cloacae DSM 30060 DSM is a member of Enterobacter cloacae complex Enterobacter cloacae DSM 30062 DSM is a member of Enterobacter cloacae complex Enterobacter cloacae DSM 6234 DSM is a member of Enterobacter cloacae complex Enterobacter cloacae MB11506_1 CHB is a member of Enterobacter cloacae complex Enterobacter cloacae MB_5277_05 THL is a member of Enterobacter cloacae complex Enterobacter cloacae MB_8779_05 THL is a member of Enterobacter cloacae complex Enterobacter hormaechei ssp hormaechei DSM 12409T DSM is a member of Enterobacter cloacae complex Enterobacter kobei DSM 13645T DSM is a member of Enterobacter cloacae complex Matching Hints table--continued on next page Report created at 2021-08-09T12:26:41 Research Use Only Page 5 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Matching Hints table--continued from previous page Matched Pattern Comment Enterobacter ludwigii DSM 16688T DSM is a member of Enterobacter cloacae complex Enterobacter xiangfangensis DSM 101093T DSM is a member of Enterobacter cloacae complex Enterobacter xiangfangensis DSM 46348 DSM is a member of Enterobacter cloacae complex Escherichia coli ATCC 25922 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 25922 THL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ATCC 35218 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli B421 UFL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DH5alpha BRL closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1103_QC DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 1576 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 30083T HAM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli DSM 682 DSM closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli ESBL_EA_RSS_1528T CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli MB11464_1 CHB closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli Nissl VML closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Escherichia coli RV412_A1_2010_06a LBK closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment Matching Hints table--continued on next page Report created at 2021-08-09T12:26:41 Research Use Only Page 6 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella pneumoniae 37585 PFM closely related to Klebsiella variicola Klebsiella pneumoniae RV_BA_03_B LBK closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM closely related to Klebsiella variicola Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM closely related to Klebsiella variicola Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM closely related to Klebsiella variicola Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM closely related to Klebsiella variicola Klebsiella variicola 150416_01 BRUS closely related to Klebsiella pneumoniae Klebsiella variicola 150519_01 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 150519_03 SAH closely related to Klebsiella pneumoniae Klebsiella variicola 37924 PFM closely related to Klebsiella pneumoniae Klebsiella variicola CC_2053 MCW closely related to Klebsiella pneumoniae Klebsiella variicola CC_2123 MCW closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T DSM closely related to Klebsiella pneumoniae Klebsiella variicola DSM 15968T HAM closely related to Klebsiella pneumoniae Matching Hints table--continued on next page Report created at 2021-08-09T12:26:41 Research Use Only Page 7 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Matching Hints table--continued from previous page Matched Pattern Comment Klebsiella variicola NY_1036 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1064 MCW closely related to Klebsiella pneumoniae Klebsiella variicola NY_1125 MCW closely related to Klebsiella pneumoniae Klebsiella variicola TC_4131 MCW closely related to Klebsiella pneumoniae Macrococcus caseolyticus 5D3_QSA IBS For the species canis / caseolyticus of the genus Macrococcus the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Serratia ficaria 17271986_1 MVD For the species ficaria / vespertilionis of the genus Serratia the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Serratia marcescens (PX) 24086109 MLD Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens 13103_1 CHB Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens DSM 12481 DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens DSM 12483 DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens DSM 12485 DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens DSM 30122 DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens ssp marcescens DSM 30121T DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia marcescens ssp sakuensis CIP 107489T HAM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Serratia ureilytica DSM 16952T DSM Species marcescens / ureilytica of the genus Serratia have very similar patterns: Therefore distinguishing their species is difficult. Matching Hints table--continued on next page Report created at 2021-08-09T12:26:41 Research Use Only Page 8 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Matching Hints table--continued from previous page Matched Pattern Comment Staphylococcus condimenti DSM 11675 DSM For the species carnosus / condimenti / piscifermentans of the genus Staphylococcus the 16S rRNA gene sequences are very similar. Therefore distinguishing the mentioned species is difficult. Report created at 2021-08-09T12:26:41 Research Use Only Page 9 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Meaning of Score Values Range Interpretation Symbols Color 2.00 - 3.00 High-confidence identification (+++) green 1.70 - 1.99 Low-confidence identification (+) yellow 0.00 - 1.69 No Organism Identification Possible (-) red Meaning of Consistency Categories (A - C) Category Interpretation (A) High consistency: The best match is a high-confidence identification. The second-best match is (1) a high-confidence identification in which the species is identical to the best match, (2) a low-confidence identification in which the species or genus is identical to the best match, or (3) a non-identification. (B) Low consistency: The requirements for high consistency are not met. The best match is a high- or low- confidence identification. The second-best match is (1) a high- or low-confidence identification in which the genus is identical to the best match or (2) a non-identification. (C) No consistency: The requirements for high or low consistency are not met. Report created at 2021-08-09T12:26:41 Research Use Only Page 10 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 1 Sample Name: E1 Sample Description: Sample ID: A 15166 Sample Creation Date/Time: 2021-08-09T10:57:22.836 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.91 72407 2 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.85 574 3 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.79 72407 4 (+) Klebsiella pneumoniae 37585 PFM 1.77 573 5 (+) Klebsiella variicola CC_2123 MCW 1.76 244366 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.75 72407 7 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.75 573 8 (-) Klebsiella variicola NY_1036 MCW 1.69 244366 9 (-) Klebsiella variicola NY_1125 MCW 1.69 244366 10 (-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.68 574 Report created at 2021-08-09T12:26:41 Research Use Only Page 11 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 2 Sample Name: E2 Sample Description: Sample ID: A 15246 Sample Creation Date/Time: 2021-08-09T10:57:22.836 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 1576 DSM 2.42 562 2 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.38 562 3 (+++) Escherichia coli DSM 682 DSM 2.34 562 4 (+++) Escherichia coli ATCC 25922 THL 2.23 562 5 (+++) Escherichia coli DH5alpha BRL 2.22 562 6 (+++) Escherichia coli MB11464_1 CHB 2.14 562 7 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.12 562 8 (+++) Escherichia coli ATCC 25922 CHB 2.05 562 9 (+++) Escherichia coli Nissl VML 2.03 562 10 (+) Escherichia coli DSM 1103_QC DSM 1.98 562 Report created at 2021-08-09T12:26:41 Research Use Only Page 12 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 3 Sample Name: E3 Sample Description: Sample ID: A 15247 Sample Creation Date/Time: 2021-08-09T10:57:22.837 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella pneumoniae RV_BA_03_B LBK 2.00 573 2 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.98 72407 3 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.95 574 4 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.91 72407 5 (+) Klebsiella variicola NY_1125 MCW 1.90 244366 6 (+) Klebsiella variicola CC_2123 MCW 1.89 244366 7 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.88 39831 8 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.86 72407 9 (+) Klebsiella variicola DSM 15968T DSM 1.84 244366 10 (+) Klebsiella variicola CC_2053 MCW 1.84 244366 Report created at 2021-08-09T12:26:41 Research Use Only Page 13 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 4 Sample Name: E4 Sample Description: Sample ID: C 16508-1 Sample Creation Date/Time: 2021-08-09T10:57:22.837 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae 13159_1 CHB 2.19 550 2 (+++) Enterobacter xiangfangensis DSM 46348 DSM 2.12 547 3 (+++) Enterobacter cloacae MB11506_1 CHB 2.10 550 4 (+) Enterobacter xiangfangensis DSM 101093T DSM 1.98 547 5 (+) Enterobacter cloacae MB_8779_05 THL 1.89 550 6 (+) Enterobacter cloacae MB_5277_05 THL 1.89 550 7 (+) Enterobacter cloacae DSM 30062 DSM 1.80 550 8 (+) Enterobacter cloacae DSM 30060 DSM 1.78 550 9 (-) Enterobacter hormaechei ssp hormaechei DSM 12409T DSM 1.68 301105 10 (-) Enterobacter kobei DSM 13645T DSM 1.67 208224 Report created at 2021-08-09T12:26:41 Research Use Only Page 14 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 5 Sample Name: E5 Sample Description: Sample ID: C 16508-2 Sample Creation Date/Time: 2021-08-09T10:57:22.838 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae MB_5277_05 THL 2.18 550 2 (+++) Enterobacter xiangfangensis DSM 101093T DSM 2.18 547 3 (+++) Enterobacter cloacae MB11506_1 CHB 2.15 550 4 (+++) Enterobacter cloacae MB_8779_05 THL 2.10 550 5 (+++) Enterobacter cloacae 13159_1 CHB 2.05 550 6 (+++) Enterobacter xiangfangensis DSM 46348 DSM 2.03 547 7 (+) Enterobacter hormaechei ssp hormaechei DSM 12409T DSM 1.82 301105 8 (+) Enterobacter cloacae DSM 30060 DSM 1.70 550 9 (-) Enterobacter ludwigii DSM 16688T DSM 1.69 299767 10 (-) Escherichia coli ATCC 25922 CHB 1.67 562 Report created at 2021-08-09T12:26:41 Research Use Only Page 15 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 6 Sample Name: E6 Sample Description: Sample ID: C 16499 Sample Creation Date/Time: 2021-08-09T10:57:22.838 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.96 72407 2 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.86 573 3 (+) Klebsiella pneumoniae 37585 PFM 1.85 573 4 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.84 72407 5 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.81 72407 6 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.77 574 7 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.77 39831 8 (-) Klebsiella variicola DSM 15968T DSM 1.68 244366 9 (-) Klebsiella variicola CC_2123 MCW 1.68 244366 10 (-) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.65 574 Report created at 2021-08-09T12:26:41 Research Use Only Page 16 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 7 Sample Name: E7 Sample Description: Sample ID: C 16498-KLPN Sample Creation Date/Time: 2021-08-09T10:57:22.839 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.87 72407 2 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.83 39831 3 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.83 573 4 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.77 574 5 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.74 72407 6 (+) Klebsiella variicola DSM 15968T DSM 1.74 244366 7 (+) Klebsiella pneumoniae ssp ozaenae CCM 5792T CCM 1.71 574 8 (+) Klebsiella variicola 150416_01 BRUS 1.70 244366 9 (+) Klebsiella variicola CC_2123 MCW 1.70 244366 10 (-) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.69 72407 Report created at 2021-08-09T12:26:41 Research Use Only Page 17 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 8 Sample Name: E8 Sample Description: Sample ID: C 16498-ESCO Sample Creation Date/Time: 2021-08-09T10:57:22.839 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 682 DSM 2.35 562 2 (+++) Escherichia coli DSM 1576 DSM 2.32 562 3 (+++) Escherichia coli ATCC 25922 THL 2.24 562 4 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.21 562 5 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.21 562 6 (+++) Escherichia coli MB11464_1 CHB 2.20 562 7 (+++) Escherichia coli DH5alpha BRL 2.19 562 8 (+++) Escherichia coli ATCC 25922 CHB 2.09 562 9 (+++) Escherichia coli DSM 30083T HAM 2.08 562 10 (+++) Escherichia coli ATCC 35218 CHB 2.02 562 Report created at 2021-08-09T12:26:41 Research Use Only Page 18 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 9 Sample Name: E9 Sample Description: Sample ID: C 16485 Sample Creation Date/Time: 2021-08-09T10:57:22.840 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Klebsiella variicola 150519_03 SAH 2.00 244366 2 (+) Klebsiella variicola 150416_01 BRUS 1.99 244366 3 (+) Klebsiella variicola NY_1125 MCW 1.97 244366 4 (+) Klebsiella variicola TC_4131 MCW 1.96 244366 5 (+) Klebsiella variicola DSM 15968T DSM 1.91 244366 6 (+) Klebsiella variicola CC_2123 MCW 1.90 244366 7 (+) Klebsiella variicola 150519_01 SAH 1.86 244366 8 (+) Klebsiella variicola NY_1036 MCW 1.84 244366 9 (+) Klebsiella variicola CC_2053 MCW 1.83 244366 10 (+) Klebsiella variicola NY_1064 MCW 1.82 244366 Report created at 2021-08-09T12:26:41 Research Use Only Page 19 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 10 Sample Name: E10 Sample Description: Sample ID: C 16482 Sample Creation Date/Time: 2021-08-09T10:57:22.840 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Serratia marcescens (PX) 24086109 MLD 1.95 615 2 (+) Serratia marcescens 13103_1 CHB 1.87 615 3 (+) Serratia marcescens ssp sakuensis CIP 107489T HAM 1.79 211760 4 (-) Serratia marcescens ssp marcescens DSM 30121T DSM 1.65 211759 5 (-) Serratia marcescens DSM 30122 DSM 1.60 615 6 (-) Serratia ureilytica DSM 16952T DSM 1.60 300181 7 (-) Serratia marcescens DSM 12485 DSM 1.56 615 8 (-) Serratia entomophila DSM 12358T DSM 1.49 42906 9 (-) Serratia ficaria 17271986_1 MVD 1.41 61651 10 (-) Serratia marcescens DSM 12481 DSM 1.35 615 Report created at 2021-08-09T12:26:41 Research Use Only Page 20 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 11 Sample Name: E11 Sample Description: Sample ID: C 16457 Sample Creation Date/Time: 2021-08-09T10:57:22.841 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.91 574 2 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.89 72407 3 (+) Klebsiella pneumoniae RV_BA_03_B LBK 1.83 573 4 (+) Klebsiella variicola DSM 15968T HAM 1.82 244366 5 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.75 72407 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.74 72407 7 (+) Klebsiella variicola NY_1125 MCW 1.71 244366 8 (-) Klebsiella variicola CC_2053 MCW 1.69 244366 9 (-) Klebsiella variicola CC_2123 MCW 1.67 244366 10 (-) Klebsiella variicola 37924 PFM 1.64 244366 Report created at 2021-08-09T12:26:41 Research Use Only Page 21 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 12 Sample Name: E12 Sample Description: Sample ID: C 16485 Sample Creation Date/Time: 2021-08-09T10:57:22.842 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.95 287 2 (+) Pseudomonas aeruginosa ATCC 27853 THL 1.80 287 3 (+) Pseudomonas aeruginosa 19955_1 CHB 1.79 287 4 (+) Pseudomonas aeruginosa 8147_2 CHB 1.79 287 5 (-) Pseudomonas aeruginosa A07_08_Pudu FLR 1.52 287 6 (-) Lactobacillus salivarius DSM 20492 DSM 1.51 1624 7 (-) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.45 287 8 (-) Pseudomonas aeruginosa DSM 1117 DSM 1.43 287 9 (-) Staphylococcus condimenti DSM 11675 DSM 1.40 70255 10 (-) Pseudomonas resinovorans LMG 2274T HAM 1.39 53412 Report created at 2021-08-09T12:26:41 Research Use Only Page 22 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 13 Sample Name: F1 Sample Description: Sample ID: C 16457 Sample Creation Date/Time: 2021-08-09T10:57:22.842 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Pseudomonas aeruginosa ATCC 27853 THL 1.85 287 2 (+) Pseudomonas aeruginosa 8147_2 CHB 1.79 287 3 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.73 287 4 (-) Pseudomonas aeruginosa 19955_1 CHB 1.51 287 5 (-) Pseudomonas aeruginosa A07_08_Pudu FLR 1.50 287 6 (-) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.38 287 7 (-) Acinetobacter baumannii B389 UFL 1.37 470 8 (-) Pseudomonas citronellolis DSM 50332T HAM 1.35 53408 9 (-) Pseudomonas aeruginosa DSM 1117 DSM 1.35 287 10 (-) Pseudomonas nitroreducens LMG 20221T HAM 1.29 46680 Report created at 2021-08-09T12:26:41 Research Use Only Page 23 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 14 Sample Name: F2 Sample Description: Sample ID: A 15295 Sample Creation Date/Time: 2021-08-09T10:57:22.843 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli DSM 682 DSM 2.34 562 2 (+++) Escherichia coli DSM 1576 DSM 2.31 562 3 (+++) Escherichia coli ATCC 25922 THL 2.26 562 4 (+++) Escherichia coli MB11464_1 CHB 2.23 562 5 (+++) Escherichia coli DH5alpha BRL 2.17 562 6 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.16 562 7 (+++) Escherichia coli ESBL_EA_RSS_1528T CHB 2.14 562 8 (+++) Escherichia coli ATCC 25922 CHB 2.10 562 9 (+++) Escherichia coli DSM 30083T HAM 2.04 562 10 (+++) Escherichia coli DSM 1103_QC DSM 2.00 562 Report created at 2021-08-09T12:26:41 Research Use Only Page 24 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 15 Sample Name: F3 Sample Description: Sample ID: A 15319-ESMA Sample Creation Date/Time: 2021-08-09T10:57:22.843 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli B421 UFL 2.10 562 2 (+++) Escherichia coli ATCC 35218 CHB 2.08 562 3 (+++) Escherichia coli DSM 30083T HAM 2.07 562 4 (+++) Escherichia coli Nissl VML 2.06 562 5 (+++) Escherichia coli MB11464_1 CHB 2.06 562 6 (+++) Escherichia coli DSM 1576 DSM 2.05 562 7 (+++) Escherichia coli RV412_A1_2010_06a LBK 2.04 562 8 (+++) Escherichia coli ATCC 25922 THL 2.00 562 9 (+) Escherichia coli DSM 682 DSM 1.96 562 10 (+) Escherichia coli ESBL_EA_RSS_1528T CHB 1.92 562 Report created at 2021-08-09T12:26:41 Research Use Only Page 25 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 16 Sample Name: F4 Sample Description: Sample ID: A 15319 Sample Creation Date/Time: 2021-08-09T10:57:22.844 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Enterobacter cloacae 13159_1 CHB 1.94 550 2 (+) Enterobacter cloacae MB_5277_05 THL 1.88 550 3 (+) Enterobacter cloacae MB_8779_05 THL 1.86 550 4 (+) Enterobacter xiangfangensis DSM 101093T DSM 1.83 547 5 (+) Enterobacter cloacae MB11506_1 CHB 1.80 550 6 (+) Enterobacter cloacae 20105_2 CHB 1.70 550 7 (-) Enterobacter hormaechei ssp hormaechei DSM 12409T DSM 1.67 301105 8 (-) Enterobacter xiangfangensis DSM 46348 DSM 1.63 547 9 (-) Citrobacter koseri Mu15167_1 CHB 1.52 545 10 (-) Enterobacter cloacae DSM 30060 DSM 1.52 550 Report created at 2021-08-09T12:26:41 Research Use Only Page 26 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 17 Sample Name: F5 Sample Description: Sample ID: A 15329 Sample Creation Date/Time: 2021-08-09T10:57:22.844 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Escherichia coli MB11464_1 CHB 2.07 562 2 (+) Escherichia coli ATCC 25922 THL 1.97 562 3 (+) Escherichia coli Nissl VML 1.91 562 4 (+) Escherichia coli DSM 682 DSM 1.91 562 5 (+) Escherichia coli DSM 30083T HAM 1.90 562 6 (+) Escherichia coli RV412_A1_2010_06a LBK 1.88 562 7 (+) Escherichia coli DH5alpha BRL 1.86 562 8 (+) Escherichia coli ATCC 35218 CHB 1.84 562 9 (+) Escherichia coli DSM 1576 DSM 1.83 562 10 (+) Escherichia coli B421 UFL 1.82 562 Report created at 2021-08-09T12:26:41 Research Use Only Page 27 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 18 Sample Name: F6 Sample Description: Sample ID: B 15405-KLOX Sample Creation Date/Time: 2021-08-09T10:57:22.845 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+) Klebsiella pneumoniae ssp ozaenae DSM 16358T HAM 1.97 574 2 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T HAM 1.95 72407 3 (+) Klebsiella variicola CC_2053 MCW 1.92 244366 4 (+) Klebsiella variicola CC_2123 MCW 1.86 244366 5 (+) Klebsiella pneumoniae ssp pneumoniae 9295_1 CHB 1.85 72407 6 (+) Klebsiella pneumoniae ssp pneumoniae DSM 30104T_QC DSM 1.82 72407 7 (+) Klebsiella variicola NY_1125 MCW 1.82 244366 8 (+) Klebsiella pneumoniae ssp rhinoscleromatis DSM 16231T HAM 1.80 39831 9 (+) Klebsiella variicola 150519_03 SAH 1.78 244366 10 (+) Klebsiella variicola DSM 15968T DSM 1.78 244366 Report created at 2021-08-09T12:26:41 Research Use Only Page 28 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 19 Sample Name: F7 Sample Description: Sample ID: B 15405-CIFR Sample Creation Date/Time: 2021-08-09T10:57:22.845 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Citrobacter freundii 22054_1 CHB 2.30 546 2 (+++) Citrobacter freundii DSM 30039T HAM 2.21 546 3 (+++) Citrobacter freundii 13158_2 CHB 2.09 546 4 (+++) Citrobacter freundii LMG 21265 LMG 2.09 546 5 (+++) Citrobacter freundii LMG 3251 LMG 2.09 546 6 (+++) Citrobacter freundii DSM 15979 DSM 2.08 546 7 (+++) Citrobacter freundii DSM 30039T DSM 2.07 546 8 (+) Citrobacter youngae DSM 17578T HAM 1.97 133448 9 (+) Citrobacter braakii 9314_2 CHB 1.93 57706 10 (+) Citrobacter gillenii CCUG 2749 CCUG 1.92 67828 Report created at 2021-08-09T12:26:41 Research Use Only Page 29 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 20 Sample Name: F8 Sample Description: Sample ID: C 15614 Sample Creation Date/Time: 2021-08-09T10:57:22.846 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Serratia marcescens (PX) 24086109 MLD 2.12 615 2 (+) Serratia marcescens 13103_1 CHB 1.99 615 3 (+) Serratia marcescens ssp marcescens DSM 30121T DSM 1.90 211759 4 (+) Serratia marcescens ssp sakuensis CIP 107489T HAM 1.89 211760 5 (+) Serratia marcescens DSM 12485 DSM 1.77 615 6 (+) Serratia marcescens DSM 30122 DSM 1.72 615 7 (-) Serratia marcescens DSM 12483 DSM 1.63 615 8 (-) Serratia marcescens DSM 12481 DSM 1.60 615 9 (-) Macrococcus caseolyticus 5D3_QSA IBS 1.54 69966 10 (-) Serratia ureilytica DSM 16952T DSM 1.51 300181 Report created at 2021-08-09T12:26:41 Research Use Only Page 30 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 21 Sample Name: F9 Sample Description: Sample ID: C 15644 Sample Creation Date/Time: 2021-08-09T10:57:22.846 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae 13159_1 CHB 2.07 550 2 (+) Enterobacter cloacae MB11506_1 CHB 1.99 550 3 (+) Enterobacter xiangfangensis DSM 101093T DSM 1.96 547 4 (+) Enterobacter xiangfangensis DSM 46348 DSM 1.95 547 5 (+) Enterobacter cloacae DSM 30060 DSM 1.94 550 6 (+) Enterobacter cloacae MB_5277_05 THL 1.87 550 7 (+) Enterobacter cloacae MB_8779_05 THL 1.86 550 8 (+) Enterobacter cloacae DSM 30062 DSM 1.70 550 9 (-) Escherichia coli ATCC 25922 CHB 1.45 562 10 (-) Enterobacter cloacae DSM 6234 DSM 1.44 550 Report created at 2021-08-09T12:26:41 Research Use Only Page 31 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 22 Sample Name: F10 Sample Description: Sample ID: C 16659-1 Sample Creation Date/Time: 2021-08-09T10:57:22.847 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae MB_5277_05 THL 2.24 550 2 (+++) Enterobacter xiangfangensis DSM 101093T DSM 2.23 547 3 (+++) Enterobacter cloacae MB11506_1 CHB 2.22 550 4 (+++) Enterobacter xiangfangensis DSM 46348 DSM 2.17 547 5 (+++) Enterobacter cloacae 13159_1 CHB 2.11 550 6 (+++) Enterobacter cloacae MB_8779_05 THL 2.09 550 7 (+) Enterobacter hormaechei ssp hormaechei DSM 12409T DSM 1.93 301105 8 (+) Enterobacter cloacae DSM 30062 DSM 1.87 550 9 (+) Enterobacter cloacae 20105_2 CHB 1.87 550 10 (+) Enterobacter bugandensis DSM 101088 DSM 1.83 547 Report created at 2021-08-09T12:26:42 Research Use Only Page 32 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 23 Sample Name: F11 Sample Description: Sample ID: C 16659-2 Sample Creation Date/Time: 2021-08-09T10:57:22.847 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae 20105_2 CHB 2.26 550 2 (+++) Enterobacter ludwigii DSM 16688T DSM 2.16 299767 3 (+) Enterobacter asburiae DSM 17506T DSM 1.95 61645 4 (+) Enterobacter cloacae MB11506_1 CHB 1.94 550 5 (+) Enterobacter bugandensis DSM 29888T DSM 1.92 547 6 (+) Enterobacter bugandensis DSM 101086 DSM 1.90 547 7 (+) Enterobacter bugandensis DSM 101087 DSM 1.90 547 8 (+) Enterobacter cloacae 13159_1 CHB 1.88 550 9 (+) Enterobacter bugandensis DSM 101088 DSM 1.88 547 10 (+) Enterobacter bugandensis DSM 101089 DSM 1.88 547 Report created at 2021-08-09T12:26:42 Research Use Only Page 33 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 24 Sample Name: F12 Sample Description: Sample ID: C 16661-OT Sample Creation Date/Time: 2021-08-09T10:57:22.848 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based B on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Enterobacter cloacae 20105_2 CHB 2.24 550 2 (+++) Enterobacter ludwigii DSM 16688T DSM 2.09 299767 3 (+++) Enterobacter asburiae CCM 4032 CCM 2.04 61645 4 (+) Enterobacter cloacae MB11506_1 CHB 1.93 550 5 (+) Enterobacter bugandensis DSM 101087 DSM 1.92 547 6 (+) Enterobacter bugandensis DSM 101091 DSM 1.92 547 7 (+) Enterobacter bugandensis DSM 101092 DSM 1.91 547 8 (+) Enterobacter cloacae DSM 30062 DSM 1.90 550 9 (+) Enterobacter bugandensis DSM 101089 DSM 1.89 547 10 (+) Enterobacter bugandensis DSM 101090 DSM 1.88 547 Report created at 2021-08-09T12:26:42 Research Use Only Page 34 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 25 Sample Name: G1 Sample Description: Sample ID: B 15405-1 Sample Creation Date/Time: 2021-08-09T10:57:22.849 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.05 287 2 (+) Pseudomonas aeruginosa 8147_2 CHB 1.94 287 3 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.93 287 4 (+) Pseudomonas aeruginosa 19955_1 CHB 1.91 287 5 (-) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.65 287 6 (-) Pseudomonas aeruginosa A07_08_Pudu FLR 1.62 287 7 (-) Pseudomonas aeruginosa LMG 8029 LMG 1.45 287 8 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.42 287 9 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.41 198616 10 (-) Pseudomonas aeruginosa DSM 1117 DSM 1.34 287 Report created at 2021-08-09T12:26:42 Research Use Only Page 35 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 26 Sample Name: G2 Sample Description: Sample ID: B 15406 Sample Creation Date/Time: 2021-08-09T10:57:22.849 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.36 287 2 (+++) Pseudomonas aeruginosa 19955_1 CHB 2.24 287 3 (+++) Pseudomonas aeruginosa DSM 50071T_QC DSM 2.17 287 4 (+++) Pseudomonas aeruginosa 8147_2 CHB 2.16 287 5 (+++) Pseudomonas aeruginosa DSM 50071T HAM 2.10 287 6 (+++) Pseudomonas aeruginosa DSM 1117 DSM 2.06 287 7 (+) Pseudomonas aeruginosa DSM 1128 DSM 1.86 287 8 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.82 287 9 (+) Pseudomonas aeruginosa LMG 8029 LMG 1.77 287 10 (-) Pseudomonas jinjuensis LMG 21316T HAM 1.60 198616 Report created at 2021-08-09T12:26:42 Research Use Only Page 36 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 27 Sample Name: G3 Sample Description: Sample ID: C 15614 Sample Creation Date/Time: 2021-08-09T10:57:22.850 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.09 287 2 (+) Pseudomonas aeruginosa 19955_1 CHB 1.94 287 3 (+) Pseudomonas aeruginosa DSM 50071T HAM 1.93 287 4 (+) Pseudomonas aeruginosa DSM 1117 DSM 1.92 287 5 (+) Pseudomonas aeruginosa 8147_2 CHB 1.91 287 6 (+) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.85 287 7 (+) Pseudomonas aeruginosa A07_08_Pudu FLR 1.71 287 8 (-) Pseudomonas aeruginosa LMG 8029 LMG 1.67 287 9 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.54 287 10 (-) Pseudomonas fragi DSM 3456T HAM 1.29 296 Report created at 2021-08-09T12:26:42 Research Use Only Page 37 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 28 Sample Name: G4 Sample Description: Sample ID: B 15405-2 Sample Creation Date/Time: 2021-08-09T10:57:22.850 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based A on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (+++) Pseudomonas aeruginosa DSM 50071T HAM 2.14 287 2 (+++) Pseudomonas aeruginosa ATCC 27853 THL 2.12 287 3 (+++) Pseudomonas aeruginosa 8147_2 CHB 2.06 287 4 (+) Pseudomonas aeruginosa 19955_1 CHB 1.96 287 5 (-) Pseudomonas aeruginosa DSM 1117 DSM 1.68 287 6 (-) Pseudomonas aeruginosa LMG 8029 LMG 1.67 287 7 (-) Pseudomonas aeruginosa DSM 1128 DSM 1.67 287 8 (-) Pseudomonas aeruginosa DSM 50071T_QC DSM 1.55 287 9 (-) Pseudomonas nitroreducens LMG 20221T HAM 1.44 46680 10 (-) Pseudomonas aeruginosa A07_08_Pudu FLR 1.41 287 Report created at 2021-08-09T12:26:42 Research Use Only Page 38 of 39 Run Identifier: 210809-1051-10184 Run Creation Date/Time: 2021-08-09T12:20:23.005 Sample 29 Sample Name: G5 Sample Description: Sample ID: C XXX Sample Creation Date/Time: 2021-08-09T10:57:22.851 Sample Type: Standard Identification Method: MALDI Biotyper MSP Identification Standard Method 1.1 Preprocessing Method: MALDI Biotyper Preprocessing Standard Method 1.1 ACQ Method: D:\Methods\flexControlMethods\MBT_FC.par AutoXecute Method: MBT_AutoX Consistency Category (based C on 2 best matches): Applied Taxonomy Tree: Bruker Taxonomy Rank (Quality) Matched Pattern Score Value NCBI Identifier 1 (-) no peaks found 0.00 - 2 (-) no peaks found 0.00 - 3 (-) no peaks found 0.00 - 4 (-) no peaks found 0.00 - 5 (-) no peaks found 0.00 - 6 (-) no peaks found 0.00 - 7 (-) no peaks found 0.00 - 8 (-) no peaks found 0.00 - 9 (-) no peaks found 0.00 - 10 (-) no peaks found 0.00 - Report created at 2021-08-09T12:26:42 Research Use Only Page 39 of 39